BMRB Entry 16773

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PDB ID: 2kvb
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16773
MolProbity Validation Chart

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Title:
SOLUTION STRUCTURE OF CI-MPR domain 5 bound to N-acetylglucosaminyl 6-phosphomethylmannoside
Deposition date:
2010-03-10
Original release date:
2012-08-03
Authors:
Olson, L.; Peterson, F.; Volkman, B.; Dahms, N.
Citation:

Citation: Olson, L.; Peterson, F.; Castonguay, A.; Bohnsack, R.; Kudo, M.; Gotschall, R.; Canfield, W.; Volkman, B.; Dahms, N.. "Structural basis for recognition of phosphodiester-containing lysosomal enzymes by the cation-independent mannose 6-phosphate receptor."  Proc. Natl. Acad. Sci. U. S. A. 107, 12493-12498 (2010).
PubMed: 20615935

Assembly members:

Assembly members:
CI-MPR_domain5, polymer, 148 residues, 16759.471 Da.

Natural source:

Natural source:   Common Name: cow   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Pichia pastoris   Vector: pPICZ-alpha-A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CI-MPR_domain5: EAEAEFLSRTEGDNCTVFDS QAGFSFDLTPLTKKDAYKVE TDKYEFHINVCGPVSVGACP PDSGACQVSRSDRKSWNLGR SNAKLSYYDGMIQLTYRDGT PYNNEKRTPRATLITFLCDR DAGVGFPEYQEEDNSTYNFR WYTSYACP

Data sets:
Data typeCount
13C chemical shifts570
15N chemical shifts153
1H chemical shifts897

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1CI-MPR domain51

Entities:

Entity 1, CI-MPR domain5 148 residues - 16759.471 Da.

Residues 578-583 are a cloning artifact.

1   GLUALAGLUALAGLUPHELEUSERARGTHR
2   GLUGLYASPASNCYSTHRVALPHEASPSER
3   GLNALAGLYPHESERPHEASPLEUTHRPRO
4   LEUTHRLYSLYSASPALATYRLYSVALGLU
5   THRASPLYSTYRGLUPHEHISILEASNVAL
6   CYSGLYPROVALSERVALGLYALACYSPRO
7   PROASPSERGLYALACYSGLNVALSERARG
8   SERASPARGLYSSERTRPASNLEUGLYARG
9   SERASNALALYSLEUSERTYRTYRASPGLY
10   METILEGLNLEUTHRTYRARGASPGLYTHR
11   PROTYRASNASNGLULYSARGTHRPROARG
12   ALATHRLEUILETHRPHELEUCYSASPARG
13   ASPALAGLYVALGLYPHEPROGLUTYRGLN
14   GLUGLUASPASNSERTHRTYRASNPHEARG
15   TRPTYRTHRSERTYRALACYSPRO

Related Database Links:

BMRB 16772
PDB 2KVA 2KVB

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