BMRB Entry 16772

Name: SOLUTION STRUCTURE OF CI-MPR ligand-free domain 5
Published: 2012-08-03

Click here to enlarge.

PDB ID: 2kva
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16772
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

SOLUTION STRUCTURE OF CI-MPR ligand-free domain 5

Deposition date:

2010-03-10

Original release date:

2012-08-03

Authors:

Olson, L.; Peterson, F.; Volkman, B.; Dahms, N.

Citation:

Citation: Olson, L.; Peterson, F.; Castonguay, A.; Bohnsack, R.; Kudo, M.; Gotschall, R.; Canfield, W.; Volkman, B.; Dahms, N.. "Structural basis for recognition of phosphodiester-containing lysosomal enzymes by the cation-independent mannose 6-phosphate receptor." Proc. Natl. Acad. Sci. U. S. A. 107, 12493-12498 (2010).
PubMed: 20615935

Assembly members:

Assembly members:
CI-MPR_domain5, polymer, 148 residues, 16759.471 Da.

Natural source:

Natural source: Common Name: cow Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos taurus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Pichia pastoris Vector: pPICZ-alpha-A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CI-MPR_domain5: EAEAEFLSRTEGDNCTVFDS QAGFSFDLTPLTKKDAYKVE TDKYEFHINVCGPVSVGACP PDSGACQVSRSDRKSWNLGR SNAKLSYYDGMIQLTYRDGT PYNNEKRTPRATLITFLCDR DAGVGFPEYQEEDNSTYNFR WYTSYACP

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	569
15N chemical shifts	151
1H chemical shifts	888

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CI-MPR domain5	1

Entities:

Entity 1, CI-MPR domain5 148 residues - 16759.471 Da.

Residues 578-583 are a cloning artifact.

1

GLU

ALA

GLU

ALA

GLU

PHE

LEU

SER

ARG

THR

2

GLU

GLY

ASP

ASN

CYS

THR

VAL

PHE

ASP

SER

3

GLN

ALA

GLY

PHE

SER

PHE

ASP

LEU

THR

PRO

4

LEU

THR

LYS

ASP

ALA

TYR

LYS

VAL

GLU

5

THR

ASP

LYS

TYR

GLU

PHE

HIS

ILE

ASN

VAL

6

CYS

GLY

PRO

VAL

SER

VAL

GLY

ALA

CYS

PRO

7

PRO

ASP

SER

GLY

ALA

CYS

GLN

VAL

SER

ARG

8

SER

ASP

ARG

LYS

SER

TRP

ASN

LEU

GLY

ARG

9

SER

ASN

ALA

LYS

LEU

SER

TYR

ASP

GLY

10

MET

ILE

GLN

LEU

THR

TYR

ARG

ASP

GLY

THR

11

PRO

TYR

ASN

GLU

LYS

ARG

THR

PRO

ARG

12

ALA

THR

LEU

ILE

THR

PHE

LEU

CYS

ASP

ARG

13

ASP

ALA

GLY

VAL

GLY

PHE

PRO

GLU

TYR

GLN

14

GLU

ASP

ASN

SER

THR

TYR

ASN

PHE

ARG

15

TRP

TYR

THR

SER

TYR

ALA

CYS

PRO

Samples:

sample: CI-MPR domain5, [U-100% 13C; U-100% 15N], 1 mM; bis-tris, [U-2H], 10 mM; sodium chloride 150 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D_15N-separated_NOESY	sample	isotropic	sample_conditions_1
3D_13C-separated_NOESY	sample	isotropic	sample_conditions_1
3D_13C-separated_NOESY (AROMATIC)	sample	isotropic	sample_conditions_1

Software:

X-PLOR NIH v2.9.3, SCHWIETERS,C.D.,KUSZEWSKI,J.J.,TJANDRA,N.,CLORE,G.M. - refinement

TOPSPIN v2.1, Bruker - collection

NMRPipe v2007, Delagio,F. et al. - processing

XEASY v1.3, Eccles, C., Guntert, P., Billeter, M., Wuthrich, K. - data analysis

GARANT v2.1, C. Bartels - data analysis

CYANA v2.1, Guntert, P. - structural calculation

NMR spectrometers:

Bruker Avance 600 MHz