BMRB Entry 16634

Name: Backbone Dynamics and Global Effects of an Activity Enhancing Mutation in Minimized Mtu RecA Inteins
Published: 2010-06-28

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16634

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone Dynamics and Global Effects of an Activity Enhancing Mutation in Minimized Mtu RecA Inteins

Deposition date:

2009-12-15

Original release date:

2010-06-28

Authors:

Du, Zhenming; Liu, Yangzhong; Ban, David; Lopez, Maria; Belfort, Marlene; Wang, Chunyu

Citation:

Citation: Du, Zhenming; Liu, Yangzhong; Ban, David; Lopez, Maria; Belfort, Marlene; Wang, Chunyu. "Backbone dynamics and global effects of an activating mutation in minimized Mtu RecA inteins." J. Mol. Biol. 400, 755-767 (2010).
PubMed: 20562025

Assembly members:

Assembly members:
Mtu_RecA_intein, polymer, 139 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mycobacterium tuberculosis Taxonomy ID: 1773 Superkingdom: Bacteria Kingdom: not available Genus/species: Mycobacterium tuberculosis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pXI

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Mtu_RecA_intein: CLAEGTRIFDPVTGTTHRIE DVVGGRKPIHVVAAAKDGTL HARPVVSWFDQGTRDVIGLR IAGGAIVWATPDHKVLTEYG WRAAGELRKGDRVAVRDVET GELRYSVIREVLPTRRARTF GLEVEELHTLVAEGVVVHN

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	132
1H chemical shifts	132

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ddIhh-V67CM	1

Entities:

Entity 1, ddIhh-V67CM 139 residues - Formula weight is not available

1

CYS

LEU

ALA

GLU

GLY

THR

ARG

ILE

PHE

ASP

2

PRO

VAL

THR

GLY

THR

HIS

ARG

ILE

GLU

3

ASP

VAL

GLY

ARG

LYS

PRO

ILE

HIS

4

VAL

ALA

LYS

ASP

GLY

THR

LEU

5

HIS

ALA

ARG

PRO

VAL

SER

TRP

PHE

ASP

6

GLN

GLY

THR

ARG

ASP

VAL

ILE

GLY

LEU

ARG

7

ILE

ALA

GLY

ALA

ILE

VAL

TRP

ALA

THR

8

PRO

ASP

HIS

LYS

VAL

LEU

THR

GLU

TYR

GLY

9

TRP

ARG

ALA

GLY

GLU

LEU

ARG

LYS

GLY

10

ASP

ARG

VAL

ALA

VAL

ARG

ASP

VAL

GLU

THR

11

GLY

GLU

LEU

ARG

TYR

SER

VAL

ILE

ARG

GLU

12

VAL

LEU

PRO

THR

ARG

ALA

ARG

THR

PHE

13

GLY

LEU

GLU

VAL

GLU

LEU

HIS

THR

LEU

14

VAL

ALA

GLU

GLY

VAL

HIS

ASN

Samples:

sample_1: Mtu RecA intein, [U-99% 13C; U-99% 15N], 0.15 – 0.3 mM; H2O 90%; D2O 10%; NaH2PO4/Na2HPO4 50 mM; NaCl 100 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

SPARKY, Goddard - chemical shift assignment

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

Bruker DRX 800 MHz

BMRB	15560 17414
PDB	2IN0 2IN8 2IN9 2L8L 3IFJ 3IGD
EMBL	CFR77613 CMM98736
GB	EPZ65824 KAM73204
REF	WP_049963343