BMRB Entry 16616

Name: assignment for the I214V variant of rabbit prion protein (91-228)
Published: 2010-10-13

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16616

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

assignment for the I214V variant of rabbit prion protein (91-228)

Deposition date:

2009-11-24

Original release date:

2010-10-13

Authors:

Li, Jun; Wen, Yi; Lin, Donghai

Citation:

Citation: Wen, Yi; Li, Jun; Xiong, Minqian; Peng, Yu; Yao, Wenming; Hong, Jing; Lin, Donghai. "Solution Structure and Dynamics of the I214V Mutant of the Rabbit Prion Protein." PLoS ONE 5, e13273-. (2010).
PubMed: 20949107

Assembly members:

Assembly members:
prion, polymer, 145 residues, Formula weight is not available

Natural source:

Natural source: Common Name: rabbit Taxonomy ID: 9986 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Oryctolagus cuniculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET30a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
prion: MQGGTHNQWGKPSKPKTSMK HVAGAAAAGAVVGGLGGYML GSAMSRPLIHFGNDYEDRYY RENMYRYPNQVYYRPVDQYS NQNSFVHDCVNITVKQHTVT TTTKGENFTETDIKIMERVV EQMCVTQYQQESQAAYQRAH HHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	362
15N chemical shifts	139
1H chemical shifts	874

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	peptide chain	1

Entities:

Entity 1, peptide chain 145 residues - Formula weight is not available

The first residue (M) and the last six residues (His tag) come from the vector of pET30a.

1

MET

GLN

GLY

THR

HIS

ASN

GLN

TRP

GLY

2

LYS

PRO

SER

LYS

PRO

LYS

THR

SER

MET

LYS

3

HIS

VAL

ALA

GLY

ALA

GLY

ALA

4

VAL

GLY

LEU

GLY

TYR

MET

LEU

5

GLY

SER

ALA

MET

SER

ARG

PRO

LEU

ILE

HIS

6

PHE

GLY

ASN

ASP

TYR

GLU

ASP

ARG

TYR

7

ARG

GLU

ASN

MET

TYR

ARG

TYR

PRO

ASN

GLN

8

VAL

TYR

ARG

PRO

VAL

ASP

GLN

TYR

SER

9

ASN

GLN

ASN

SER

PHE

VAL

HIS

ASP

CYS

VAL

10

ASN

ILE

THR

VAL

LYS

GLN

HIS

THR

VAL

THR

11

THR

LYS

GLY

GLU

ASN

PHE

THR

GLU

12

THR

ASP

ILE

LYS

ILE

MET

GLU

ARG

VAL

13

GLU

GLN

MET

CYS

VAL

THR

GLN

TYR

GLN

14

GLU

SER

GLN

ALA

TYR

GLN

ARG

ALA

HIS

15

HIS

Samples:

sample_1: prion, [U-100% 13C; U-100% 15N], 1 mM; H2O 90%; D2O 10%; sodium acetate 20 mM

sample_conditions_1: ionic strength: 0.04 M; pH: 4.5; pressure: 1 atm; temperature: 298.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Varian Unity 600 MHz

BMRB	15394 15399 16328
PDB	2FJ3 2JOH 2JOM 3O79 4HLS 4HMM 4HMR
GB	AAC48697 AAD01554 ABL75505 AEM44421 AEM44422
REF	NP_001075490 XP_008254357 XP_008254358
SP	Q95211
AlphaFold	Q95211