BMRB Entry 15399

Name: NMR structure of rabbit prion protein mutation I214V
Published: 2010-10-19

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PDB ID: 2jom
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15399
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR structure of rabbit prion protein mutation I214V

Deposition date:

2007-07-20

Original release date:

2010-10-19

Authors:

Li, Jun; Lin, Donghai

Citation:

Citation: Wen, Yi; Li, Jun; Xiong, Minqian; Peng, Yu; Yao, Wenming; Hong, Jing; Lin, Donghai. "Solution Structure and Dynamics of the I214V Mutant of the Rabbit Prion Protein." PLoS ONE 5, e13273-. (2010).
PubMed: 20949107

Assembly members:

Assembly members:
rpp I214V, polymer, 138 residues, 12471.928 Da.

Natural source:

Natural source: Common Name: rabbit Taxonomy ID: 9986 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Oryctolagus cuniculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-30a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
rpp I214V: QGGTHNQWGKPSKPKTSMKH VAGAAAAGAVVGGLGGYMLG SAMSRPLIHFGNDYEDRYYR ENMYRYPNQVYYRPVDQYSN QNSFVHDCVNITVKQHTVTT TTKGENFTETDIKIMERVVE QMCVTQYQQESQAAYQRA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	532
15N chemical shifts	156
1H chemical shifts	926

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	rpp I214V	1

Entities:

Entity 1, rpp I214V 138 residues - 12471.928 Da.

1

GLN

GLY

THR

HIS

ASN

GLN

TRP

GLY

LYS

2

PRO

SER

LYS

PRO

LYS

THR

SER

MET

LYS

HIS

3

VAL

ALA

GLY

ALA

GLY

ALA

VAL

4

VAL

GLY

LEU

GLY

TYR

MET

LEU

GLY

5

SER

ALA

MET

SER

ARG

PRO

LEU

ILE

HIS

PHE

6

GLY

ASN

ASP

TYR

GLU

ASP

ARG

TYR

ARG

7

GLU

ASN

MET

TYR

ARG

TYR

PRO

ASN

GLN

VAL

8

TYR

ARG

PRO

VAL

ASP

GLN

TYR

SER

ASN

9

GLN

ASN

SER

PHE

VAL

HIS

ASP

CYS

VAL

ASN

10

ILE

THR

VAL

LYS

GLN

HIS

THR

VAL

THR

11

THR

LYS

GLY

GLU

ASN

PHE

THR

GLU

THR

12

ASP

ILE

LYS

ILE

MET

GLU

ARG

VAL

GLU

13

GLN

MET

CYS

VAL

THR

GLN

TYR

GLN

GLU

14

SER

GLN

ALA

TYR

GLN

ARG

ALA

Samples:

sample_1: rpp I214V, [U-13C; U-15N], 0.7 – 0.8 mM; sodium acetate 20 mM

sample_conditions_1: pH: 4.5; pressure: 1 atm; temperature: 298.13 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

ARIA, Linge, O'Donoghue and Nilges - structure solution

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Varian INOVA 600 MHz

BMRB	15394 16328 16616
PDB	2FJ3 2JOH 2JOM 3O79 4HLS 4HMM 4HMR
GB	AAC48697 AAD01554 ABL75505 AEM44421 AEM44422
REF	NP_001075490 XP_008254357 XP_008254358
SP	Q95211
AlphaFold	Q95211