BMRB Entry 16409

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR16409

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Title:
Backbone 1H, 15N, and 13C assignments for beta phosphoglucomutase in a ternary complex with alpha-galactose 1-phosphate and MgF3-
Deposition date:
2009-07-16
Original release date:
2009-11-16
Authors:
Baxter, Nicola; Waltho, Jonathan
Citation:

Citation: Baxter, Nicola; Hounslow, Andrea; Bowler, Matthew; Williams, Nicholas; Blackburn, G. Michael; Waltho, Jonathan. "MgF(3)(-) and alpha-Galactose 1-Phosphate in the Active Site of beta-Phosphoglucomutase Form a Transition State Analogue of Phosphoryl Transfer."  J. Am. Chem. Soc. 131, 16334-16335 (2009).
PubMed: 19852484

Assembly members:

Assembly members:
beta_phosphoglucomutase, polymer, 221 residues, Formula weight is not available
GL1, non-polymer, 260.136 Da.
MGF, non-polymer, 81.300 Da.
MG, non-polymer, 24.305 Da.

Natural source:

Natural source:   Common Name: Lactococcus lactis   Taxonomy ID: 1358   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Lactococcus lactis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
beta_phosphoglucomutase: MFKAVLFDLDGVITDTAEYH FRAWKALAEEIGINGVDRQF NEQLKGVSREDSLQKILDLA DKKVSAEEFKELAKRKNDNY VKMIQDVSPADVYPGILQLL KDLRSNKIKIALASASKNGP FLLERMNLTGYFDAIADPAE VAASKPAPDIFIAAAHAVGV APSESIGLEDSQAGIQAIKD SGALPIGVGRPEDLGDDIVI VPDTSHYTLEFLKEVWLQKQ K

Data sets:
Data typeCount
13C chemical shifts415
15N chemical shifts210
19F chemical shifts3
1H chemical shifts210

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1beta phosphoglucomutase1
2alpha-galactose 1-phosphate2
3MgF3-3
4Mg4

Entities:

Entity 1, beta phosphoglucomutase 221 residues - Formula weight is not available

1   METPHELYSALAVALLEUPHEASPLEUASP
2   GLYVALILETHRASPTHRALAGLUTYRHIS
3   PHEARGALATRPLYSALALEUALAGLUGLU
4   ILEGLYILEASNGLYVALASPARGGLNPHE
5   ASNGLUGLNLEULYSGLYVALSERARGGLU
6   ASPSERLEUGLNLYSILELEUASPLEUALA
7   ASPLYSLYSVALSERALAGLUGLUPHELYS
8   GLULEUALALYSARGLYSASNASPASNTYR
9   VALLYSMETILEGLNASPVALSERPROALA
10   ASPVALTYRPROGLYILELEUGLNLEULEU
11   LYSASPLEUARGSERASNLYSILELYSILE
12   ALALEUALASERALASERLYSASNGLYPRO
13   PHELEULEUGLUARGMETASNLEUTHRGLY
14   TYRPHEASPALAILEALAASPPROALAGLU
15   VALALAALASERLYSPROALAPROASPILE
16   PHEILEALAALAALAHISALAVALGLYVAL
17   ALAPROSERGLUSERILEGLYLEUGLUASP
18   SERGLNALAGLYILEGLNALAILELYSASP
19   SERGLYALALEUPROILEGLYVALGLYARG
20   PROGLUASPLEUGLYASPASPILEVALILE
21   VALPROASPTHRSERHISTYRTHRLEUGLU
22   PHELEULYSGLUVALTRPLEUGLNLYSGLN
23   LYS

Entity 2, alpha-galactose 1-phosphate - C6 H13 O9 P - 260.136 Da.

1   GL1

Entity 3, MgF3- - F3 Mg - 81.300 Da.

1   MGF

Entity 4, Mg - Mg - 24.305 Da.

1   MG

Related Database Links:

PDB 1z4o 2wf5 1o08 1z4n 1o03 1LVH 1O03 1O08 1Z4N 1Z4O 1ZOL 2WF5 2WF6 2WF7 2WF8 2WF9 2WFA 2WHE 3FM9 3ZI4 4C4R 4C4S 4C4T
BMRB 7235 15467 7234 15467 17851 17852
DBJ BAL50396 GAM81375
EMBL CAA94734 CDG03643 CDI46177
GB AAK04527 ADA64250 ADZ63078 AEE43918 AGY43735
REF NP_266585 WP_003131530 WP_010905331 WP_012897250 WP_021469610
SP P71447
AlphaFold P71447

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks