BMRB Entry 7235

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7235

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Backbone 1H, 15N, and 13C assignments for beta phosphoglucomutase in the open form
Deposition date:
2006-07-13
Original release date:
2006-09-08
Authors:
Baxter, Nicola; Waltho, Jonathan
Citation:

Citation: Baxter, Nicola; Olguin, Luis; Golicnik, Marko; Feng, Guoqiang; Hounslow, Andrea; Bermel, Wolfgang; Blackburn, G.; Hollfelder, Florian; Waltho, Jonathan; Williams, Nicholas. "A trojan horse transition state analogue generated by MgF3- formation in an enzyme active site"  Proc. Natl. Acad. Sci. U.S.A. 103, 14732-14737 (2006).
PubMed: 16990434

Assembly members:

Assembly members:
beta phosphoglucomutase, polymer, 221 residues, 25000 Da.
MG, non-polymer, 24.305 Da.

Natural source:

Natural source:   Common Name: Lactococcus lactis   Taxonomy ID: 1360   Superkingdom: Eubacteria   Kingdom: Metazoa   Genus/species: Lactococcus lactis

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
beta phosphoglucomutase: MFKAVLFDLDGVITDTAEYH FRAWKALAEEIGINGVDRQF NEQLKGVSREDSLQKILDLA DKKVSAEEFKELAKRKNDNY VKMIQDVSPADVYPGILQLL KDLRSNKIKIALASASKNGP FLLERMNLTGYFDAIADPAE VAASKPAPDIFIAAAHAVGV APSESIGLEDSQAGIQAIKD SGALPIGVGRPEDLGDDIVI VPDTSHYTLEFLKEVWLQKQ K

Data sets:
Data typeCount
13C chemical shifts568
15N chemical shifts180
1H chemical shifts180

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1beta phosphoglucomutase1
2magnesium ion2

Entities:

Entity 1, beta phosphoglucomutase 221 residues - 25000 Da.

1   METPHELYSALAVALLEUPHEASPLEUASP
2   GLYVALILETHRASPTHRALAGLUTYRHIS
3   PHEARGALATRPLYSALALEUALAGLUGLU
4   ILEGLYILEASNGLYVALASPARGGLNPHE
5   ASNGLUGLNLEULYSGLYVALSERARGGLU
6   ASPSERLEUGLNLYSILELEUASPLEUALA
7   ASPLYSLYSVALSERALAGLUGLUPHELYS
8   GLULEUALALYSARGLYSASNASPASNTYR
9   VALLYSMETILEGLNASPVALSERPROALA
10   ASPVALTYRPROGLYILELEUGLNLEULEU
11   LYSASPLEUARGSERASNLYSILELYSILE
12   ALALEUALASERALASERLYSASNGLYPRO
13   PHELEULEUGLUARGMETASNLEUTHRGLY
14   TYRPHEASPALAILEALAASPPROALAGLU
15   VALALAALASERLYSPROALAPROASPILE
16   PHEILEALAALAALAHISALAVALGLYVAL
17   ALAPROSERGLUSERILEGLYLEUGLUASP
18   SERGLNALAGLYILEGLNALAILELYSASP
19   SERGLYALALEUPROILEGLYVALGLYARG
20   PROGLUASPLEUGLYASPASPILEVALILE
21   VALPROASPTHRSERHISTYRTHRLEUGLU
22   PHELEULYSGLUVALTRPLEUGLNLYSGLN
23   LYS

Entity 2, magnesium ion - Mg - 24.305 Da.

1   MG

Samples:

sample_open_2H15N13C: beta phosphoglucomutase open conformation, [U-2H; U-15N; U-13C], 1 ± 0.2 mM; magnesium chloride 5 ± 0.2 mM; ammonium fluoride 10 ± 0.2 mM; sodium azide 2 ± 0.2 mM; deuterium oxide 15 ± 1 %; protease inhibitor 1 times; potassium HEPES 50 ± 2 mM

conditions_1: pH: 7.2; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N TROSYsample_open_2H15N13Cnot availableconditions_1
TROSY ct-HNCAsample_open_2H15N13Cnot availableconditions_1
TROSY ct-HN(CO)CAsample_open_2H15N13Cnot availableconditions_1
TROSY HN(CA)CBsample_open_2H15N13Cnot availableconditions_1
TROSY HN(COCA)CBsample_open_2H15N13Cnot availableconditions_1
TROSY HN(CA)COsample_open_2H15N13Cnot availableconditions_1
TROSY HNCOsample_open_2H15N13Cnot availableconditions_1
{19F}1H,15N-HSQC NOE differencesample_open_2H15N13Cnot availableconditions_1
19F 1Dsample_open_2H15N13Cnot availableconditions_1

Software:

xwinnmr, Bruker BioSpin Ltd - data acquisition

FELIX v2004, Accelrys Inc - data analysis, data processing

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 15467 16409 17851 17852 7234
PDB
DBJ BAL50396
EMBL CAA94734 CDG03643 CDI46177
GB AAK04527 ADA64250 ADZ63078 AEE43918 AGY43735
REF NP_266585 WP_003131530 WP_010905331 WP_012897250 WP_021469610
SP P71447
AlphaFold P71447

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks