BMRB Entry 16328

Name: the S173N variant of rabbit prion protein (91-228)
Published: 2010-07-20

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16328

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

the S173N variant of rabbit prion protein (91-228)

Deposition date:

2009-05-30

Original release date:

2010-07-20

Authors:

Li, Jun; Wen, Yi; Lin, Donghai

Citation:

Citation: Wen, Yi; Li, Jun; Yao, Wenming; Xiong, Minqian; Hong, Jing; Peng, Yu; Xiao, Gengfu; Lin, Donghai. "Unique structural characteristics of the rabbit prion protein." J. Biol. Chem. 285, 31682-31693 (2010).
PubMed: 20639199

Assembly members:

Assembly members:
prion_protein, polymer, 145 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Rabbit Taxonomy ID: 9986 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Oryctolagus cuniculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET30a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
prion_protein: MQGGTHNQWGKPSKPKTSMK HVAGAAAAGAVVGGLGGYML GSAMSRPLIHFGNDYEDRYY RENMYRYPNQVYYRPVDQYS NQNNFVHDCVNITVKQHTVT TTTKGENFTETDIKIMERVV EQMCITQYQQESQAAYQRAH HHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list

Data type	Count
13C chemical shifts	315
15N chemical shifts	126
1H chemical shifts	714

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	prion protein	1

Entities:

Entity 1, prion protein 145 residues - Formula weight is not available

1

MET

GLN

GLY

THR

HIS

ASN

GLN

TRP

GLY

2

LYS

PRO

SER

LYS

PRO

LYS

THR

SER

MET

LYS

3

HIS

VAL

ALA

GLY

ALA

GLY

ALA

4

VAL

GLY

LEU

GLY

TYR

MET

LEU

5

GLY

SER

ALA

MET

SER

ARG

PRO

LEU

ILE

HIS

6

PHE

GLY

ASN

ASP

TYR

GLU

ASP

ARG

TYR

7

ARG

GLU

ASN

MET

TYR

ARG

TYR

PRO

ASN

GLN

8

VAL

TYR

ARG

PRO

VAL

ASP

GLN

TYR

SER

9

ASN

GLN

ASN

PHE

VAL

HIS

ASP

CYS

VAL

10

ASN

ILE

THR

VAL

LYS

GLN

HIS

THR

VAL

THR

11

THR

LYS

GLY

GLU

ASN

PHE

THR

GLU

12

THR

ASP

ILE

LYS

ILE

MET

GLU

ARG

VAL

13

GLU

GLN

MET

CYS

ILE

THR

GLN

TYR

GLN

14

GLU

SER

GLN

ALA

TYR

GLN

ARG

ALA

HIS

15

HIS

Samples:

sample: prion_protein, [U-100% 13C; U-100% 15N], 1 mM; sodium acetate 20 mM; H2O 90%; D2O 10%

sample_condition: ionic strength: 0.04 M; pH: 4.5; pressure: 1 atm; temperature: 298.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
standard NMR experiments	sample	isotropic	sample_condition

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Varian INOVA 600 MHz

BMRB	15394 15399 16616
PDB	1TPX 1TQB 1TQC 2FJ3 2JOH 2JOM 3O79 4HLS 4HMM 4HMR
EMBL	CBK44083
GB	AAC48697 AAD01554 ABL75505 AEM44421 AEM44422
REF	NP_001075490 XP_008254357 XP_008254358
SP	Q95211
AlphaFold	Q95211