BMRB Entry 16183

Title:
Tammar Wallaby Prion Protein (121-230)
Deposition date:
2009-02-24
Original release date:
2009-05-20
Authors:
Christen, Barbara; Hornemann, Simone; Damberger, Fred; Wuthrich, Kurt
Citation:

Citation: Christen, Barbara; Hornemann, Simone; Damberger, Fred; Wuthrich, Kurt. "Prion protein NMR structure from tammar wallaby (Macropus eugenii) shows that the beta2-alpha2 loop is modulated by long-range sequence effects."  J. Mol. Biol. 389, 833-845 (2009).
PubMed: 19393664

Assembly members:

Assembly members:
wpp_121-230, polymer, 112 residues, 13167.715 Da.

Natural source:

Natural source:   Common Name: tammar wallaby   Taxonomy ID: 9315   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Macropus Eugenii

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pRSETA

Data sets:
Data typeCount
13C chemical shifts473
15N chemical shifts132
1H chemical shifts782

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1wpp_121-2301

Entities:

Entity 1, wpp_121-230 112 residues - 13167.715 Da.

1   GLYSERVALVALGLYGLYLEUGLYGLYTYR
2   METLEUGLYSERALAMETSERARGPROVAL
3   METHISPHEGLYASNGLUTYRGLUASPARG
4   TYRTYRARGGLUASNGLNTYRARGTYRPRO
5   ASNGLNVALMETTYRARGPROILEASPGLN
6   TYRGLYSERGLNASNSERPHEVALHISASP
7   CYSVALASNILETHRVALLYSGLNHISTHR
8   THRTHRTHRTHRTHRLYSGLYGLUASNPHE
9   THRGLUTHRASPILELYSILEMETGLUARG
10   VALVALGLUGLNMETCYSILETHRGLNTYR
11   GLNASNGLUTYRGLNALAALAGLNARGTYR
12   TYRASN

Samples:

sample_1: sodium acetate, [U-100% 2H], 10 mM; entity, [U-99% 13C; U-99% 15N], 1.5 mM; sodium azide 0.02%

sample_conditions_1: ionic strength: 0.01 M; pH: 4.5; pressure: 1 atm; temperature: 293.1 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D H(CCCO)NHsample_1isotropicsample_conditions_1
3D (H)C(CCO)NHsample_1isotropicsample_conditions_1

Software:

CARA, Keller and Wuthrich - data analysis

ATHNOS-CANDID v1.2, Herrmann, Guntert and Wuthrich - chemical shift assignment, peak picking

DYANA v1.0.3, Guntert, Mumenthaler and Wuthrich - structure solution

OPALP, Luginbuhl, Guntert, Billeter and Wuthrich - refinement

Molmol, Koradi, Billeter and Wuthrich - data analysis

TOPSPIN, Bruker Biospin - collection, processing

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 600 MHz
  • Bruker Avance 750 MHz
  • Bruker Avance 900 MHz

Related Database Links:

PDB
GB AAT68001 AAT68002

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks