BMRB Entry 15944

Name: Structural Basis for the Regulation of p53 Function by p300
Published: 2009-03-12

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PDB ID: 2k8f
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15944
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structural Basis for the Regulation of p53 Function by p300

Deposition date:

2008-09-08

Original release date:

2009-03-12

Authors:

Bai, Yawen; Feng, Hanqiao; Jenkins, Lisa; Durell, Stewart; Wiodawer, Alex; Appella, Ettore

Citation:

Citation: Feng, Hanqiao; Miller Jenkins, Lisa; Durell, Stewart; Hayashi, Ryo; Mazur, Sharlyn; Cherry, Scott; Tropea, Joseph; Miller, Maria; Wlodawer, Alexander; Appella, Ettore; Bai, Yawen. "Structural Basis for p300 Taz2-p53 TAD1 Binding and Modulation by Phosphorylation" Structure 17, 202-210 (2009).
PubMed: 19217391

Assembly members:

Assembly members:
TAZ2, polymer, 90 residues, 9963.838 Da.
TAD(1-39), polymer, 39 residues, 4351.815 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pDONR201 to make pJT57

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TAZ2: ATQSPGDSRRLSIQRAIQSL VHAAQCRNANCSLPSCQKMK RVVQHTKGCKRKTNGGCPIC KQLIALAAYHAKHCQENKCP VPFCLNIKQK
TAD(1-39): MEEPQSDPSVEPPLSQETFS DLWKLLPENNVLSPLPSQA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	506
15N chemical shifts	111
1H chemical shifts	751

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TAZ2	1
2	TAD(1-39)	2

Entities:

Entity 1, TAZ2 90 residues - 9963.838 Da.

1-90 from p300(1723-1812)

1	ALA	THR	GLN	SER	PRO	GLY	ASP	SER	ARG	ARG
2	LEU	SER	ILE	GLN	ARG	ALA	ILE	GLN	SER	LEU
3	VAL	HIS	ALA	ALA	GLN	CYS	ARG	ASN	ALA	ASN
4	CYS	SER	LEU	PRO	SER	CYS	GLN	LYS	MET	LYS
5	ARG	VAL	VAL	GLN	HIS	THR	LYS	GLY	CYS	LYS
6	ARG	LYS	THR	ASN	GLY	GLY	CYS	PRO	ILE	CYS
7	LYS	GLN	LEU	ILE	ALA	LEU	ALA	ALA	TYR	HIS
8	ALA	LYS	HIS	CYS	GLN	GLU	ASN	LYS	CYS	PRO
9	VAL	PRO	PHE	CYS	LEU	ASN	ILE	LYS	GLN	LYS

Entity 2, TAD(1-39) 39 residues - 4351.815 Da.

1-39

1

MET

GLU

PRO

GLN

SER

ASP

PRO

SER

VAL

2

GLU

PRO

LEU

SER

GLN

GLU

THR

PHE

SER

3

ASP

LEU

TRP

LYS

LEU

PRO

GLU

ASN

4

VAL

LEU

SER

PRO

LEU

PRO

SER

GLN

ALA

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	TAZ2cpx_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	TAZ2cpx_4	isotropic	sample_conditions_1
3D CBCA(CO)NH	TAZ2cpx_2	isotropic	sample_conditions_1
3D HNCACB	TAZ2cpx_2	isotropic	sample_conditions_1
3D HNCO	TAZ2cpx_2	isotropic	sample_conditions_1
3D HN(CA)CO	TAZ2cpx_2	isotropic	sample_conditions_1
3D HNCA	TAZ2cpx_2	isotropic	sample_conditions_1
3D H(CCO)NH	TAZ2cpx_2	isotropic	sample_conditions_1
3D HCCH-TOCSY	TAZ2cpx_2	isotropic	sample_conditions_1
3D HNHA	TAZ2cpx_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	TAZ2cpx_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	TAZ2cpx_2	isotropic	sample_conditions_1
2D 1H-15N HSQC	p53cpx_3	isotropic	sample_conditions_1
3D CBCA(CO)NH	p53cpx_3	isotropic	sample_conditions_1
3D HNCACB	p53cpx_3	isotropic	sample_conditions_1
3D HNCA	p53cpx_3	isotropic	sample_conditions_1
3D HNCO	p53cpx_3	isotropic	sample_conditions_1
3D HN(CA)CO	p53cpx_3	isotropic	sample_conditions_1
3D H(CCO)NH	p53cpx_3	isotropic	sample_conditions_1
3D HCCH-TOCSY	p53cpx_3	isotropic	sample_conditions_1
3D 1H-15N NOESY	p53cpx_3	isotropic	sample_conditions_1
3D 1H-13C NOESY	p53cpx_3	isotropic	sample_conditions_1

BMRB	25484 17073 17760 18709
PDB	2K8F 2MH0 2MZD 3IO2 3T92 2GS0 2K8F 2L14 2LY4 3DAC
DBJ	BAC16799 BAG35463 BAG59884 BAG60244 BAI45431
EMBL	CAA26306 CAA34420 CAA38095 CAA42625 CAA42626
GB	AAA17994 AAA59987 AAA59988 AAA59989 AAA61211
PRF	2006287A
REF	NP_000537 NP_001040616 NP_001119584 NP_001119585 NP_001119586
SP	P04637 P13481 P56423 P56424 P61260
AlphaFold	P04637 P13481 P56423 P56424 P61260

BMRB Entry 15944

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	ALA	THR	GLN	SER	PRO	GLY	ASP	SER	ARG	ARG
2	LEU	SER	ILE	GLN	ARG	ALA	ILE	GLN	SER	LEU
3	VAL	HIS	ALA	ALA	GLN	CYS	ARG	ASN	ALA	ASN
4	CYS	SER	LEU	PRO	SER	CYS	GLN	LYS	MET	LYS
5	ARG	VAL	VAL	GLN	HIS	THR	LYS	GLY	CYS	LYS
6	ARG	LYS	THR	ASN	GLY	GLY	CYS	PRO	ILE	CYS
7	LYS	GLN	LEU	ILE	ALA	LEU	ALA	ALA	TYR	HIS
8	ALA	LYS	HIS	CYS	GLN	GLU	ASN	LYS	CYS	PRO
9	VAL	PRO	PHE	CYS	LEU	ASN	ILE	LYS	GLN	LYS

1	ALA	THR	GLN	SER	PRO	GLY	ASP	SER	ARG	ARG
2	LEU	SER	ILE	GLN	ARG	ALA	ILE	GLN	SER	LEU
3	VAL	HIS	ALA	ALA	GLN	CYS	ARG	ASN	ALA	ASN
4	CYS	SER	LEU	PRO	SER	CYS	GLN	LYS	MET	LYS
5	ARG	VAL	VAL	GLN	HIS	THR	LYS	GLY	CYS	LYS
6	ARG	LYS	THR	ASN	GLY	GLY	CYS	PRO	ILE	CYS
7	LYS	GLN	LEU	ILE	ALA	LEU	ALA	ALA	TYR	HIS
8	ALA	LYS	HIS	CYS	GLN	GLU	ASN	LYS	CYS	PRO
9	VAL	PRO	PHE	CYS	LEU	ASN	ILE	LYS	GLN	LYS

1	ALA	THR	GLN	SER	PRO	GLY	ASP	SER	ARG	ARG
2	LEU	SER	ILE	GLN	ARG	ALA	ILE	GLN	SER	LEU
3	VAL	HIS	ALA	ALA	GLN	CYS	ARG	ASN	ALA	ASN
4	CYS	SER	LEU	PRO	SER	CYS	GLN	LYS	MET	LYS
5	ARG	VAL	VAL	GLN	HIS	THR	LYS	GLY	CYS	LYS
6	ARG	LYS	THR	ASN	GLY	GLY	CYS	PRO	ILE	CYS
7	LYS	GLN	LEU	ILE	ALA	LEU	ALA	ALA	TYR	HIS
8	ALA	LYS	HIS	CYS	GLN	GLU	ASN	LYS	CYS	PRO
9	VAL	PRO	PHE	CYS	LEU	ASN	ILE	LYS	GLN	LYS