BMRB Entry 17760

Name: backbone resonance assignments of p53 N-terminal transactivation domain (1-93)
Published: 2011-07-18

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17760

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

backbone resonance assignments of p53 N-terminal transactivation domain (1-93)

Deposition date:

2011-07-05

Original release date:

2011-07-18

Authors:

Wong, Tuck; Rutherford, Trevor; Freund, Stefan; Fersht, Alan

Citation:

Citation: Wong, Tuck; Rajagopalan, Sridharan; Freund, Stefan; Rutherford, Trevor; Andreeva, Antonina; Townsley, Fiona; Petrovich, Miriana; Fersht, Alan. "Biophysical characterizations of human mitochondrial travscription factor A and its binding to tumor suppressor p53" Nucleic Acids Res. 37, 6765-6783 (2009).
PubMed: 19755502

Assembly members:

Assembly members:
p53_TAD, polymer, 93 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: NA

Entity Sequences (FASTA):

Entity Sequences (FASTA):
p53_TAD: MEEPQSDPSVEPPLSQETFS DLWKLLPENNVLSPLPSQAM DDLMLSPDDIEQWFTEDPGP DEAPRMPEAAPPVAPAPAAP TPAAPAPAPSWPL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	251
15N chemical shifts	67
1H chemical shifts	67

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	p53_TAD	1

Entities:

Entity 1, p53_TAD 93 residues - Formula weight is not available

1

MET

GLU

PRO

GLN

SER

ASP

PRO

SER

VAL

2

GLU

PRO

LEU

SER

GLN

GLU

THR

PHE

SER

3

ASP

LEU

TRP

LYS

LEU

PRO

GLU

ASN

4

VAL

LEU

SER

PRO

LEU

PRO

SER

GLN

ALA

MET

5

ASP

LEU

MET

LEU

SER

PRO

ASP

ILE

6

GLU

GLN

TRP

PHE

THR

GLU

ASP

PRO

GLY

PRO

7

ASP

GLU

ALA

PRO

ARG

MET

PRO

GLU

ALA

8

PRO

VAL

ALA

PRO

ALA

PRO

ALA

PRO

9

THR

PRO

ALA

PRO

ALA

PRO

ALA

PRO

SER

10

TRP

PRO

LEU

Samples:

sample_1: p53_TAD, [U-100% 13C; U-100% 15N], 200 uM; TRIS 25 mM; sodium chloride 150 mM; DTT 1 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 175 mM; pH: 7.4; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

SPARKY v3.1, Goddard - data analysis

NMR spectrometers:

Bruker Avance 700 MHz

PDB	2GS0 2L14 2LY4
DBJ	BAC16799 BAG35463 BAG64357 BAI45431 BAJ52715
EMBL	CAA26306 CAA38095 CAA42625 CAA42626 CAA42627
GB	AAA59987 AAA59988 AAA59989 AAA61211 AAA61212
REF	NP_000537 NP_001119584 NP_001119585 NP_001119586 NP_001119590
SP	P04637 Q9TTA1
AlphaFold	P04637 Q9TTA1