BMRB Entry 15930
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15930
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Citation: Kaczka, Piotr; Polkowska, Agnieszka; Bolewska, Krystyna; Zhukov, Igor; Poznanski, Jaroslaw; Wierzchowski, Kazimierz. "Backbone dynamics of TFE-induced native-like fold of region 4 of Escherichia coli RNA polymerase sigma70 subunit." Proteins 78, 754-768 (2010).
PubMed: 19847776
Assembly members:
4_sigma70, polymer, 107 residues, 12032.5 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-15b
Entity Sequences (FASTA):
4_sigma70: MGSSHHHHHHSSGLVPRGSH
MLELPLDSATTESLRAATHD
VLAGLTAREAKVLRMRFGID
MNTDYTLEEVGKQFDVTRER
IRQIEAKALRKLRHPSRSEV
LRSFLDD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 279 |
| 15N chemical shifts | 101 |
| 1H chemical shifts | 346 |
| T1 relaxation values | 101 |
| T2 relaxation values | 101 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | 4 sigma70 | 1 |
Entities:
Entity 1, 4 sigma70 107 residues - 12032.5 Da.
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | HIS | ||||
| 3 | MET | LEU | GLU | LEU | PRO | LEU | ASP | SER | ALA | THR | ||||
| 4 | THR | GLU | SER | LEU | ARG | ALA | ALA | THR | HIS | ASP | ||||
| 5 | VAL | LEU | ALA | GLY | LEU | THR | ALA | ARG | GLU | ALA | ||||
| 6 | LYS | VAL | LEU | ARG | MET | ARG | PHE | GLY | ILE | ASP | ||||
| 7 | MET | ASN | THR | ASP | TYR | THR | LEU | GLU | GLU | VAL | ||||
| 8 | GLY | LYS | GLN | PHE | ASP | VAL | THR | ARG | GLU | ARG | ||||
| 9 | ILE | ARG | GLN | ILE | GLU | ALA | LYS | ALA | LEU | ARG | ||||
| 10 | LYS | LEU | ARG | HIS | PRO | SER | ARG | SER | GLU | VAL | ||||
| 11 | LEU | ARG | SER | PHE | LEU | ASP | ASP |
Related Database Links:
| BMRB | 15936 15975 |
| PDB | 1TLH 2P7V 3IYD 3T72 4JK1 4JK2 4JKR 4KMU 4KN4 4KN7 4LJZ 4LK0 4LK1 4LLG 4MEX 4MEY 4XSX 4XSY 4XSZ 4YFK 4YFN 4YFX 4YG2 4YLN 4YLO 4YLP 4ZH2 4ZH3 4ZH4 |
| DBJ | BAB37373 BAE77118 BAG66772 BAG78873 BAH65247 |
| EMBL | CAD07736 CAL34114 CAP77541 CAQ33404 CAQ90501 |
| GB | AAA24601 AAA27242 AAA89147 AAB60181 AAC76103 |
| PIR | AB0893 |
| REF | NP_311977 NP_417539 NP_457602 NP_462126 NP_708877 |
| SP | P00579 P0A2E3 P0A2E4 |
| AlphaFold | P00579 P0A2E3 P0A2E4 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks