BMRB Entry 11466

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11466

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Title:
NMR chemical shift of bryum coronatum chitinase E61A mutant
Deposition date:
2011-12-19
Original release date:
2012-11-02
Authors:
Nagata, Takuya; Shinya, Shoko; Ohnuma, Takayuki; Taira, Toki; Fukamizo, Tamo
Citation:

Citation: Nagata, Takuya; Shinya, Shoko; Ohnuma, Takayuki; Fukamizo, Tamo. "Chitin oligosaccharide binding to a family GH19 chitinase from moss, Bryum coronatumn"  Arch. Biochem. Biophys. ., .-..

Assembly members:

Assembly members:
BcChi-A_E61A, polymer, 205 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Bryum coronatum   Taxonomy ID: 216087   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Bryum coronatum

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
BcChi-A_E61A: QSWTSFVTPAVFEGWFPNRN PFYTYDGLVSASNGYPEFGT TGSLDDQKRELAAFLGNINQ ASGGLQFIQEQNPQSDYCDT SSTQYPCAAGKQYYGRGPIQ LSWNYNYGEAGADLGLDLLN NPDLVAQDSTVAWRTALWFW MKRDCHGAITASPPSFSGTI RIINGGLECNQPAGSIGNMQ MENRVTYYTQFCQTLGVDPG TDLRC

Data sets:
Data typeCount
13C chemical shifts576
15N chemical shifts191
1H chemical shifts191

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Bryum coronatum chitinase E61A mutant1

Entities:

Entity 1, Bryum coronatum chitinase E61A mutant 205 residues - Formula weight is not available

1   GLNSERTRPTHRSERPHEVALTHRPROALA
2   VALPHEGLUGLYTRPPHEPROASNARGASN
3   PROPHETYRTHRTYRASPGLYLEUVALSER
4   ALASERASNGLYTYRPROGLUPHEGLYTHR
5   THRGLYSERLEUASPASPGLNLYSARGGLU
6   LEUALAALAPHELEUGLYASNILEASNGLN
7   ALASERGLYGLYLEUGLNPHEILEGLNGLU
8   GLNASNPROGLNSERASPTYRCYSASPTHR
9   SERSERTHRGLNTYRPROCYSALAALAGLY
10   LYSGLNTYRTYRGLYARGGLYPROILEGLN
11   LEUSERTRPASNTYRASNTYRGLYGLUALA
12   GLYALAASPLEUGLYLEUASPLEULEUASN
13   ASNPROASPLEUVALALAGLNASPSERTHR
14   VALALATRPARGTHRALALEUTRPPHETRP
15   METLYSARGASPCYSHISGLYALAILETHR
16   ALASERPROPROSERPHESERGLYTHRILE
17   ARGILEILEASNGLYGLYLEUGLUCYSASN
18   GLNPROALAGLYSERILEGLYASNMETGLN
19   METGLUASNARGVALTHRTYRTYRTHRGLN
20   PHECYSGLNTHRLEUGLYVALASPPROGLY
21   THRASPLEUARGCYS

Related Database Links:

BMRB 11441
PDB 3WH1 4IJ4
DBJ BAF99002

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks