BMRB Entry 11242

Title:
Solution structure of the first SH3 domain of human vinexin
Deposition date:
2010-07-23
Original release date:
2011-08-03
Authors:
Zhang, H.; Izumi, K.; Yoshida, M.; Hayashi, F.; Yokoyama, S.
Citation:

Citation: Zhang, H.; Izumi, K.; Yoshida, M.; Hayashi, F.; Yokoyama, S.. "Solution structure of the first SH3 domain of human vinexin"  .

Assembly members:

Assembly members:
SH3 domain, residues 8-62, polymer, 68 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P050516-12

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SH3 domain, residues 8-62: GSSGSSGKAARLKFDFQAQS PKELTLQKGDIVYIHKEVDK NWLEGEHHGRLGIFPANYVE VLSGPSSG

Data sets:
Data typeCount
13C chemical shifts277
15N chemical shifts61
1H chemical shifts435

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Vinexin1

Entities:

Entity 1, Vinexin 68 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYLYSALAALA
2   ARGLEULYSPHEASPPHEGLNALAGLNSER
3   PROLYSGLULEUTHRLEUGLNLYSGLYASP
4   ILEVALTYRILEHISLYSGLUVALASPLYS
5   ASNTRPLEUGLUGLYGLUHISHISGLYARG
6   LEUGLYILEPHEPROALAASNTYRVALGLU
7   VALLEUSERGLYPROSERSERGLY

Samples:

sample_1: SH3 domain, residues 8-62, [U-13C; U-15N], 0.89 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

Delta v4.3.2, JEOL - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.04, Johnson, B.A. - data analysis

Kujira v0.93191, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntent, P. - refinement, structure solution

NMR spectrometers:

  • JEOL ECA 700 MHz

Related Database Links:

BMRB 15129
PDB
REF XP_004332006

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks