BMRB Entry 11073

Click here to enlarge.

PDB ID: 2rqf
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11073
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Solution structure of juvenile hormone binding protein from silkworm in complex with JH III
Deposition date:
2009-04-27
Original release date:
2009-10-14
Authors:
Suzuki, Rintaro; Fujimoto, Zui; Shiotsuki, Takahiro; Momma, Mitsuru; Tase, Akira; Yamazaki, Toshimasa
Citation:

Citation: Suzuki, Rintaro; Fujimoto, Zui; Shiotsuki, Takahiro; Momma, Mitsuru; Tase, Akira; Miyazawa, Mitsuhiro; Yamazaki, Toshimasa. "Structural basis for juvenile hormone perception by JHBP in haemolymph"  .

Assembly members:

Assembly members:
hJHBP, polymer, 227 residues, 24781.268 Da.
JH3, non-polymer, 266.376 Da.

Natural source:

Natural source:   Common Name: Silkworm   Taxonomy ID: 7091   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bombyx mori

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-2T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hJHBP: GSDGDALLKPCKLGDMQCLS SATEQFLEKTSKGIPQYDIW PIDPLVVTSLDVIAPSDAGI VIRFKNLNITGLKNQQISDF QMDTKAKTVLLKTKADLHIV GDIVIELTEQSKSFTGLYTA DTNVIGAVRYGYNLKNDDNG VQHFEVQPETFTCESIGEPK ITLSSDLSSALEKDSGNNSL EPDMEPLKTLRQAAICKIAE ACYISVVHNIRASAKILPAS SFFENLN

Data sets:
Data typeCount
13C chemical shifts1049
15N chemical shifts242
1H chemical shifts1635

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hJHBP1
2JH III2

Entities:

Entity 1, hJHBP 227 residues - 24781.268 Da.

Residues (-2)-(-1) represent a non-native sequence from affinity tag.

1   GLYSERASPGLYASPALALEULEULYSPRO
2   CYSLYSLEUGLYASPMETGLNCYSLEUSER
3   SERALATHRGLUGLNPHELEUGLULYSTHR
4   SERLYSGLYILEPROGLNTYRASPILETRP
5   PROILEASPPROLEUVALVALTHRSERLEU
6   ASPVALILEALAPROSERASPALAGLYILE
7   VALILEARGPHELYSASNLEUASNILETHR
8   GLYLEULYSASNGLNGLNILESERASPPHE
9   GLNMETASPTHRLYSALALYSTHRVALLEU
10   LEULYSTHRLYSALAASPLEUHISILEVAL
11   GLYASPILEVALILEGLULEUTHRGLUGLN
12   SERLYSSERPHETHRGLYLEUTYRTHRALA
13   ASPTHRASNVALILEGLYALAVALARGTYR
14   GLYTYRASNLEULYSASNASPASPASNGLY
15   VALGLNHISPHEGLUVALGLNPROGLUTHR
16   PHETHRCYSGLUSERILEGLYGLUPROLYS
17   ILETHRLEUSERSERASPLEUSERSERALA
18   LEUGLULYSASPSERGLYASNASNSERLEU
19   GLUPROASPMETGLUPROLEULYSTHRLEU
20   ARGGLNALAALAILECYSLYSILEALAGLU
21   ALACYSTYRILESERVALVALHISASNILE
22   ARGALASERALALYSILELEUPROALASER
23   SERPHEPHEGLUASNLEUASN

Entity 2, JH III - C16 H26 O3 - 266.376 Da.

1   JH3

Related Database Links:

GB AF098304 AAF19267 AAF19268 AAP41112
SWS Q9U556_BOMMO
BMRB 16021
PDB 2RQF 3A1Z 3AOS 3AOT
REF NP_001037074

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks