BMRB Entry 16021

Name: NMR assignments of juvenile hormone binding protein in complex with JH III
Published: 2009-01-28

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16021

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

NMR assignments of juvenile hormone binding protein in complex with JH III

Deposition date:

2008-11-05

Original release date:

2009-01-28

Authors:

Tase, Akira; Fujimoto, Zui; Shiotsuki, Takahiro

Citation:

Citation: Suzuki, Rintaro; Tase, Akira; Fujimoto, Zui; Shiotsuki, Takahiro; Yamazaki, Toshimasa. "NMR assignments of juvenile hormone binding protein in complex with JH III" Biomol. NMR Assignments 3, 73-76 (2009).
PubMed: 19636950

Assembly members:

Assembly members:
hJHBP, polymer, 227 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Silkworm Taxonomy ID: 7091 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bombyx mori

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-2T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hJHBP: GSDGDALLKPCKLGDMQCLS SATEQFLEKTSKGIPQYDIW PIDPLVVTSLDVIAPSDAGI VIRFKNLNITGLKNQQISDF QMDTKAKTVLLKTKADLHIV GDIVIELTEQSKSFTGLYTA DTNVIGAVRYGYNLKNDDNG VQHFEVQPETFTCESIGEPK ITLSSDLSSALEKDSGNNSL EPDMEPLKTLRQAAICKIAE ACYISVVHNIRASAKILPAS SFFENLN

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	1048
15N chemical shifts	242
1H chemical shifts	1601

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	hJHBP	1

Entities:

Entity 1, hJHBP 227 residues - Formula weight is not available

Residues (-2)-(-1) represent a non-native sequence from affinity tag.

1

GLY

SER

ASP

GLY

ASP

ALA

LEU

LYS

PRO

2

CYS

LYS

LEU

GLY

ASP

MET

GLN

CYS

LEU

SER

3

SER

ALA

THR

GLU

GLN

PHE

LEU

GLU

LYS

THR

4

SER

LYS

GLY

ILE

PRO

GLN

TYR

ASP

ILE

TRP

5

PRO

ILE

ASP

PRO

LEU

VAL

THR

SER

LEU

6

ASP

VAL

ILE

ALA

PRO

SER

ASP

ALA

GLY

ILE

7

VAL

ILE

ARG

PHE

LYS

ASN

LEU

ASN

ILE

THR

8

GLY

LEU

LYS

ASN

GLN

ILE

SER

ASP

PHE

9

GLN

MET

ASP

THR

LYS

ALA

LYS

THR

VAL

LEU

10

LEU

LYS

THR

LYS

ALA

ASP

LEU

HIS

ILE

VAL

11

GLY

ASP

ILE

VAL

ILE

GLU

LEU

THR

GLU

GLN

12

SER

LYS

SER

PHE

THR

GLY

LEU

TYR

THR

ALA

13

ASP

THR

ASN

VAL

ILE

GLY

ALA

VAL

ARG

TYR

14

GLY

TYR

ASN

LEU

LYS

ASN

ASP

ASN

GLY

15

VAL

GLN

HIS

PHE

GLU

VAL

GLN

PRO

GLU

THR

16

PHE

THR

CYS

GLU

SER

ILE

GLY

GLU

PRO

LYS

17

ILE

THR

LEU

SER

ASP

LEU

SER

ALA

18

LEU

GLU

LYS

ASP

SER

GLY

ASN

SER

LEU

19

GLU

PRO

ASP

MET

GLU

PRO

LEU

LYS

THR

LEU

20

ARG

GLN

ALA

ILE

CYS

LYS

ILE

ALA

GLU

21

ALA

CYS

TYR

ILE

SER

VAL

HIS

ASN

ILE

22

ARG

ALA

SER

ALA

LYS

ILE

LEU

PRO

ALA

SER

23

SER

PHE

GLU

ASN

LEU

ASN

Samples:

15N: hJHBP, [U-15N], 0.6-1.0 mM; JH III 0.6-1.0 mM; sodium phosphate 45 mM; H2O 90%; D2O 10%

13C_15N: hJHBP, [U-13C; U-15N], 0.6-1.0 mM; JH III 0.6-1.0 mM; sodium phosphate 45 mM; H2O 90%; D2O 10%

13C_15N_in_100%_D2O: hJHBP, [U-13C; U-15N], 0.6-1.0 mM; JH III 0.6-1.0 mM; sodium phosphate 45 mM; D2O 100%

pH_6.0_35C: pH: 6.0; pressure: 1 atm; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	15N	isotropic	pH_6.0_35C
2D 1H-13C HSQC	13C_15N_in_100%_D2O	isotropic	pH_6.0_35C
3D HNCO	13C_15N	isotropic	pH_6.0_35C
3D CBCA(CO)NH	13C_15N	isotropic	pH_6.0_35C
3D HNCA	13C_15N	isotropic	pH_6.0_35C
3D HCABGCO	13C_15N_in_100%_D2O	isotropic	pH_6.0_35C
3D HCCH-TOCSY	13C_15N_in_100%_D2O	isotropic	pH_6.0_35C
3D 15N-separated HOHAHA-HSQC	15N	isotropic	pH_6.0_35C
3D 15N-separated NOESY-HSQC	15N	isotropic	pH_6.0_35C
3D 13C/15N-separated NOESY-HSQC	13C_15N_in_100%_D2O	isotropic	pH_6.0_35C
3D 13C-separated NOESY-HSQC	13C_15N_in_100%_D2O	isotropic	pH_6.0_35C
3D 13C/13C-separated NOESY-HSQC	13C_15N_in_100%_D2O	isotropic	pH_6.0_35C

Software:

xwinnmr v3.1, Bruker Biospin - collection

NMRPipe vreleased at Feb 10, 2006, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY v3.113, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

Bruker DMX 750 MHz

GB	AF098304 AAF19267 AAF19268 AAP41112
SWS	Q9U556_BOMMO
BMRB	11073
PDB	2RQF 3A1Z 3AOS 3AOT
REF	NP_001037074