BMRB Entry 20113

Title:
1H, 13C assignments and NMR structure of temporin-SHf
Deposition date:
2009-12-10
Original release date:
2010-05-19
Authors:
Lequin, Olivier; Goasdoue, Nicole; Abbassi, Feten; Piesse, Christophe; Foulon, Thierry; Nicolas, Pierre; Ladram, Ali
Citation:

Citation: Abbassi, Feten; Lequin, Olivier; Piesse, Christophe; Goasdoue, Nicole; Foulon, Thierry; Nicolas, Pierre; Ladram, Ali. "Temporin-SHf, a New Type of Phe-rich and Hydrophobic Ultrashort Antimicrobial Peptide."  J. Biol. Chem. 285, 16880-16892 (2010).
PubMed: 20308076

Assembly members:

Assembly members:
Temporin-SHf, polymer, 8 residues, 1077.311 Da.

Natural source:

Natural source:   Common Name: Sahara frog   Taxonomy ID: 70019   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Pelophylax saharica

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Temporin-SHf: FFFLSRIX

Data sets:
Data typeCount
13C chemical shifts53
1H chemical shifts120

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Temporin-SHf1

Entities:

Entity 1, Temporin-SHf 8 residues - 1077.311 Da.

1   PHEPHEPHELEUSERARGILENFA

Samples:

sample_1: entity 2 mM; DSS 0.1 mM; DPC, [U-2H], 120 mM; H2O 90%; D2O 10%

sample_2: entity 1.4 mM; DSS 0.1 mM; SDS 80 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 5.4; pressure: 1 atm; temperature: 293 K

sample_conditions_2: pH: 5.4; pressure: 1 atm; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_2
2D 1H-1H TOCSYsample_2isotropicsample_conditions_2
2D 1H-13C HSQCsample_2isotropicsample_conditions_2

Software:

TOPSPIN v2.0, Bruker Biospin - collection

XEASY, Bartels et al. - chemical shift assignment

DYANA, Guntert, Braun and Wuthrich - structure solution

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

GB AM903076