data_4738 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Determination by High Field NMR Spectroscopy of the Longitudinal Electron Relaxation Rate in Cu(II)Plastocyanin from Anabaena variablis. ; _BMRB_accession_number 4738 _BMRB_flat_file_name bmr4738.str _Entry_type original _Submission_date 2000-06-13 _Accession_date 2000-06-14 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Ma Lixin . . 2 Led Jens J. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count 'other_data_list' 3 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2001-10-17 original author . stop_ _Original_release_date 2001-10-17 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; Determination by High Field NMR Spectroscopy of the Longitudinal Electron Relaxation Rate in Cu(II)Plastocyanin from Anabaena variablis. ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Ma Lixin . . 2 Led Jens J. . stop_ _Journal_abbreviation 'J. Am. Chem. Soc.' _Journal_volume 122 _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 7823 _Page_last 7824 _Year 2000 _Details . save_ ################################## # Molecular system description # ################################## save_system_A._v._Plastocyanin _Saveframe_category molecular_system _Mol_system_name 'Cu(II) Plastocyanin from Anabaena variabilis' _Abbreviation_common 'A. v. PCu(II)' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'plastocyanin peptide' $P_peptide Cu(II) $entity_CU Cu(I) $entity_CU1 stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state monomer _System_paramagnetic yes _System_thiol_state 'all other bound' _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_P_peptide _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 'Plastocyanin from Anabena variabilis' _Abbreviation_common Pcy _Molecular_mass 10500 _Mol_thiol_state 'all other bound' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 105 _Mol_residue_sequence ; ETYTVKLGSDKGLLVFEPAK LTIKPGDTVEFLNNKVPPHN VVFDAALNPAKSADLAKSLS HKQLLMSPGQSTSTTFPADA PAGEYTFYCEPHRGAGMVGK ITVAG ; loop_ _Residue_seq_code _Residue_label 1 GLU 2 THR 3 TYR 4 THR 5 VAL 6 LYS 7 LEU 8 GLY 9 SER 10 ASP 11 LYS 12 GLY 13 LEU 14 LEU 15 VAL 16 PHE 17 GLU 18 PRO 19 ALA 20 LYS 21 LEU 22 THR 23 ILE 24 LYS 25 PRO 26 GLY 27 ASP 28 THR 29 VAL 30 GLU 31 PHE 32 LEU 33 ASN 34 ASN 35 LYS 36 VAL 37 PRO 38 PRO 39 HIS 40 ASN 41 VAL 42 VAL 43 PHE 44 ASP 45 ALA 46 ALA 47 LEU 48 ASN 49 PRO 50 ALA 51 LYS 52 SER 53 ALA 54 ASP 55 LEU 56 ALA 57 LYS 58 SER 59 LEU 60 SER 61 HIS 62 LYS 63 GLN 64 LEU 65 LEU 66 MET 67 SER 68 PRO 69 GLY 70 GLN 71 SER 72 THR 73 SER 74 THR 75 THR 76 PHE 77 PRO 78 ALA 79 ASP 80 ALA 81 PRO 82 ALA 83 GLY 84 GLU 85 TYR 86 THR 87 PHE 88 TYR 89 CYS 90 GLU 91 PRO 92 HIS 93 ARG 94 GLY 95 ALA 96 GLY 97 MET 98 VAL 99 GLY 100 LYS 101 ILE 102 THR 103 VAL 104 ALA 105 GLY stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-10-06 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 1FA4 "Elucidation Of The Paramagnetic Relaxation Of Heteronuclei And Protons In Cu(Ii) Plastocyanin From Anabaena Variabilis" 100.00 105 100.00 100.00 2.27e-69 PDB 1NIN "Plastocyanin From Anabaena Variabilis, Nmr, 20 Structures" 100.00 105 100.00 100.00 2.27e-69 PDB 1TU2 "The Complex Of Nostoc Cytochrome F And Plastocyanin Determin With Paramagnetic Nmr. Based On The Structures Of Cytochrome F And" 100.00 105 100.00 100.00 2.27e-69 PDB 2CJ3 "Crystal Structure Of Plastocyanin From A Cyanobacterium, Anabaena Variabilis" 100.00 105 100.00 100.00 2.27e-69 PDB 2GIM "1.6 Angstrom Structure Of Plastocyanin From Anabaena Variabilis" 100.00 106 100.00 100.00 1.88e-69 DBJ BAB77782 "plastocyanin precursor [Nostoc sp. PCC 7120]" 100.00 139 100.00 100.00 3.65e-70 EMBL CAA05338 "plastocyanin [Nostoc sp. PCC 7119]" 100.00 139 100.00 100.00 3.65e-70 EMBL CAA32527 "unnamed protein product [Anabaena variabilis ATCC 29413]" 99.05 139 97.12 99.04 1.04e-67 GB AAA59364 "plastocyanin precursor [Anabaena sp.]" 100.00 139 100.00 100.00 3.65e-70 GB ABA22358 "Blue (type 1) copper domain protein [Anabaena variabilis ATCC 29413]" 99.05 139 97.12 99.04 1.04e-67 PIR CUAI "plastocyanin - Anabaena variabilis" 100.00 105 100.00 100.00 2.27e-69 PIR S06999 "plastocyanin precursor - Anabaena sp. (PCC 7937)" 99.05 139 97.12 99.04 1.04e-67 REF WP_010994435 "plastocyanin [Nostoc sp. PCC 7120]" 100.00 139 100.00 100.00 3.65e-70 SP O52830 "RecName: Full=Plastocyanin; Flags: Precursor" 100.00 139 100.00 100.00 3.65e-70 SP P0C178 "RecName: Full=Plastocyanin" 100.00 105 100.00 100.00 2.27e-69 SP P46444 "RecName: Full=Plastocyanin; Flags: Precursor" 100.00 139 100.00 100.00 3.65e-70 SP Q3M9H8 "RecName: Full=Plastocyanin; Flags: Precursor" 99.05 139 97.12 99.04 1.04e-67 stop_ save_ ############# # Ligands # ############# save_CU _Saveframe_category ligand _Mol_type "non-polymer (NON-POLYMER)" _Name_common 'COPPER (II) ION' _BMRB_code CU _PDB_code CU _Molecular_mass 63.546 _Mol_charge 2 _Mol_paramagnetic . _Mol_aromatic no _Details . loop_ _Atom_name _PDB_atom_name _Atom_type _Atom_chirality _Atom_charge _Atom_oxidation_number _Atom_unpaired_electrons CU CU CU . 2 . ? stop_ _Mol_thiol_state . _Sequence_homology_query_date . save_ save_CU1 _Saveframe_category ligand _Mol_type "non-polymer (NON-POLYMER)" _Name_common 'COPPER (I) ION' _BMRB_code CU1 _PDB_code CU1 _Molecular_mass 63.546 _Mol_charge 1 _Mol_paramagnetic . _Mol_aromatic no _Details . loop_ _Atom_name _PDB_atom_name _Atom_type _Atom_chirality _Atom_charge _Atom_oxidation_number _Atom_unpaired_electrons CU CU CU . 1 . ? stop_ _Mol_thiol_state . _Sequence_homology_query_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $P_peptide 'Anabaena variabilis' 1172 Bacteria . Anabaena variabilis stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $P_peptide 'purified from the natural source' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_one _Saveframe_category sample _Sample_type solution _Details 'The protein was fully reduced.' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $P_peptide 2.9 mM . $entity_CU1 2.9 mM . stop_ save_ save_sample_two _Saveframe_category sample _Sample_type solution _Details 'The protein was 18% oxidized with an error of 0.8%.' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $P_peptide 2.9 mM . $entity_CU1 2.38 mM . $entity_CU 0.52 mM . stop_ save_ save_sample_three _Saveframe_category sample _Sample_type solution _Details 'The protein was 15.5% oxidized with an error of 0.7%.' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $P_peptide 2.9 mM . $entity_CU1 2.45 mM . $entity_CU 0.45 mM . stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model Inova _Field_strength 500 _Details . save_ save_NMR_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model Inova _Field_strength 750 _Details . save_ ############################# # NMR applied experiments # ############################# save_unknown_1 _Saveframe_category NMR_applied_experiment _Experiment_name unknown _Sample_label . save_ save_NMR_spec_expt_dummy _Saveframe_category NMR_applied_experiment _Experiment_name unknown _BMRB_pulse_sequence_accession_number . _Details 'experiment information not available' save_ ####################### # Sample conditions # ####################### save_cond_set_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 7.50 0.02 n/a temperature 298 0.1 K stop_ save_