BMRB Entry 4354

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4354

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Title:
Solution NMR Studies of a 42kDa Escherichia coli Maltose Binding Protein/ Beta-Cyclodextrin Complex: Chemical Shift Assignments and Analysis
Deposition date:
1999-06-10
Original release date:
1999-06-10
Authors:
Gardner, Kevin; Zhang, Xiaochen; Gehring, Kalle; Kay, Lewis
Citation:

Citation: Gardner, Kevin; Zhang, Xiaochen; Gehring, Kalle; Kay, Lewis. "Solution NMR Studies of a 42kDa Escherichia coli Maltose Binding Protein/ Beta-Cyclodextrin Complex: Chemical Shift Assignments and Analysis"  J. Am. Chem. Soc. 120, 11738-11748 (1998).

Assembly members:

Assembly members:
E. coli maltose binding protein, polymer, 370 residues, 40695.2 Da.
BCD, non-polymer, 1134.984 Da.

Natural source:

Natural source:   Common Name: E.coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pmal-c0

Entity Sequences (FASTA):

Entity Sequences (FASTA):
E. coli maltose binding protein: KTEEGKLVIWINGDKGYNGL AEVGKKFEKDTGIKVTVEHP DKLEEKFPQVAATGDGPDII FWAHDRFGGYAQSGLLAEIT PDKAFQDKLYPFTWDAVRYN GKLIAYPIAVEALSLIYNKD LLPNPPKTWEEIPALDKELK AKGKSALMFNLQEPYFTWPL IAADGGYAFKYENGKYDIKD VGVDNAGAKAGLTFLVDLIK NKHMNADTDYSIAEAAFNKG ETAMTINGPWAWSNIDTSKV NYGVTVLPTFKGQPSKPFVG VLSAGINAASPNKELAKEFL ENYLLTDEGLEAVNKDKPLG AVALKSYEEELAKDPRIAAT MENAQKGEIMPNIPQMSAFW YAVRTAVINAASGRQTVDEA LKDAQTRITK

Data sets:
Data typeCount
13C chemical shifts1198
15N chemical shifts330
1H chemical shifts752

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1MBP1
2beta-cyclodextrin2

Entities:

Entity 1, MBP 370 residues - 40695.2 Da.

1   LYSTHRGLUGLUGLYLYSLEUVALILETRP
2   ILEASNGLYASPLYSGLYTYRASNGLYLEU
3   ALAGLUVALGLYLYSLYSPHEGLULYSASP
4   THRGLYILELYSVALTHRVALGLUHISPRO
5   ASPLYSLEUGLUGLULYSPHEPROGLNVAL
6   ALAALATHRGLYASPGLYPROASPILEILE
7   PHETRPALAHISASPARGPHEGLYGLYTYR
8   ALAGLNSERGLYLEULEUALAGLUILETHR
9   PROASPLYSALAPHEGLNASPLYSLEUTYR
10   PROPHETHRTRPASPALAVALARGTYRASN
11   GLYLYSLEUILEALATYRPROILEALAVAL
12   GLUALALEUSERLEUILETYRASNLYSASP
13   LEULEUPROASNPROPROLYSTHRTRPGLU
14   GLUILEPROALALEUASPLYSGLULEULYS
15   ALALYSGLYLYSSERALALEUMETPHEASN
16   LEUGLNGLUPROTYRPHETHRTRPPROLEU
17   ILEALAALAASPGLYGLYTYRALAPHELYS
18   TYRGLUASNGLYLYSTYRASPILELYSASP
19   VALGLYVALASPASNALAGLYALALYSALA
20   GLYLEUTHRPHELEUVALASPLEUILELYS
21   ASNLYSHISMETASNALAASPTHRASPTYR
22   SERILEALAGLUALAALAPHEASNLYSGLY
23   GLUTHRALAMETTHRILEASNGLYPROTRP
24   ALATRPSERASNILEASPTHRSERLYSVAL
25   ASNTYRGLYVALTHRVALLEUPROTHRPHE
26   LYSGLYGLNPROSERLYSPROPHEVALGLY
27   VALLEUSERALAGLYILEASNALAALASER
28   PROASNLYSGLULEUALALYSGLUPHELEU
29   GLUASNTYRLEULEUTHRASPGLUGLYLEU
30   GLUALAVALASNLYSASPLYSPROLEUGLY
31   ALAVALALALEULYSSERTYRGLUGLUGLU
32   LEUALALYSASPPROARGILEALAALATHR
33   METGLUASNALAGLNLYSGLYGLUILEMET
34   PROASNILEPROGLNMETSERALAPHETRP
35   TYRALAVALARGTHRALAVALILEASNALA
36   ALASERGLYARGGLNTHRVALASPGLUALA
37   LEULYSASPALAGLNTHRARGILETHRLYS

Entity 2, beta-cyclodextrin - C42 H70 O35 - 1134.984 Da.

1   BCD

Related Database Links:

BMRB 25237
PDB 1A7L 1ANF 1DMB 1EZ9 1EZO 1EZP 1FQA 1FQB 1FQC 1FQD 1HSJ 1JVX 1JVY 1JW4 1JW5 1LAX 1LLS 1MDP 1MDQ 1MG1 1MH3 1MH4 1MPB 1MPC 1MPD 1N3W 1N3X 1NL5 1NMU 1OMP 1PEB 1R6Z 1SVX 1T0K 1Y4C 1YTV 1ZIU 1ZJL 1ZKB 1ZMG 2D21 2H25 2KLF 2MV0 2N44 2N45 2NVU 2OBG 2OK2 2R6G 2V93 2VGQ 2XZ3 2ZXT 3A3C 3C4M 3CSB 3CSG 3D4C 3D4G 3DM0 3EF7 3EHS 3EHT 3EHU 3F5F 3G7V 3G7W 3H3G 3H4Z 3HPI 3HST 3IO4 3IO6 3IOR 3IOT 3IOU 3IOV 3IOW 3J9P 3KJT 3L2J 3LBS 3LC8 3MBP 3MP1 3MP6 3MP8 3MQ9 3N93 3N94 3N95 3N96 3O3U 3OAI 3OB4 3PGF 3PUV 3PUW 3PUX 3PUY 3PUZ 3PV0 3PY7 3Q25 3Q26 3Q27 3Q28 3Q29 3RLF 3RUM 3SER 3SES 3SET 3SEU 3SEV 3SEW 3SEX 3SEY 3VD8 3VFJ 3W15 3WAI 3WOA 4B3N 4BL8 4BL9 4BLA 4BLB 4BLD 4EDQ 4EGC 4EXK 4FE8 4FEB 4FEC 4FED 4GIZ 4GLI 4H1G 4IFP 4JBZ 4JKM 4KEG 4KHZ 4KI0 4KV3 4KYC 4KYD 4KYE 4LOG 4MBP 4MY2 4N4X 4NDZ 4NUF 4O4B 4OGM 4OZQ 4PE2 4PQK 4QSZ 4QVH 4R0Y 4RG5 4RWF 4RWG 4TSM 4WGI 4WJV 4WMS 4WMT 4WMU 4WMV 4WMW 4WMX 4WVG 4WVH 4WVI 4WVJ 4XAI 4XAJ 5C7R 5CL1 5DFM 5DIS
DBJ BAB38440 BAE78036 BAG79849 BAI28296 BAI33473
EMBL CAP78494 CAQ34383 CAR01012 CAR05669 CAR10711
GB AAB59056 AAB86559 AAB87675 AAC43128 AAC77004
REF NP_313044 NP_418458 NP_709885 WP_000367925 WP_000695369
SP P0AEX9 P0AEY0
AlphaFold P0AEX9 P0AEY0

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SPARKY: Backbone or all simulated peaks