BMRB Entry 4076

Title:
1H, 15N, 13C Chemical Shift Assignments of Outer Surface Protein A from Borrelia burgdorferi
Deposition date:
1997-11-26
Original release date:
1998-12-17
Authors:
Pham, Thuy-Nga; Koide, Shohei
Citation:

Citation: Pham, Thuy-Nga; Koide, Shohei. "NMR Studies of Borrelia burgdorferi OspA, a 28 kDa Protein Containing a Single-Layer Beta-sheet"  J. Biomol. NMR 11, 407-414 (1998).

Assembly members:

Assembly members:
Outer Surface Protein A, polymer, 257 residues, 27555 Da.

Natural source:

Natural source:   Common Name: spirochete   Taxonomy ID: 139   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Borrelia burgdorferi

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET9-OspA

Data sets:
Data typeCount
13C chemical shifts1041
15N chemical shifts254
1H chemical shifts930

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1OspA1

Entities:

Entity 1, OspA 257 residues - 27555 Da.

1   ALALYSGLNASNVALSERSERLEUASPGLU
2   LYSASNSERVALSERVALASPLEUPROGLY
3   GLUMETLYSVALLEUVALSERLYSGLULYS
4   ASNLYSASPGLYLYSTYRASPLEUILEALA
5   THRVALASPLYSLEUGLULEULYSGLYTHR
6   SERASPLYSASNASNGLYSERGLYVALLEU
7   GLUGLYVALLYSALAASPLYSSERLYSVAL
8   LYSLEUTHRILESERASPASPLEUGLYGLN
9   THRTHRLEUGLUVALPHELYSGLUASPGLY
10   LYSTHRLEUVALSERLYSLYSVALTHRSER
11   LYSASPLYSSERSERTHRGLUGLULYSPHE
12   ASNGLULYSGLYGLUVALSERGLULYSILE
13   ILETHRARGALAASPGLYTHRARGLEUGLU
14   TYRTHRGLYILELYSSERASPGLYSERGLY
15   LYSALALYSGLUVALLEULYSGLYTYRVAL
16   LEUGLUGLYTHRLEUTHRALAGLULYSTHR
17   THRLEUVALVALLYSGLUGLYTHRVALTHR
18   LEUSERLYSASNILESERLYSSERGLYGLU
19   VALSERVALGLULEUASNASPTHRASPSER
20   SERALAALATHRLYSLYSTHRALAALATRP
21   ASNSERGLYTHRSERTHRLEUTHRILETHR
22   VALASNSERLYSLYSTHRLYSASPLEUVAL
23   PHETHRLYSGLUASNTHRILETHRVALGLN
24   GLNTYRASPSERASNGLYTHRLYSLEUGLU
25   GLYSERALAVALGLUILETHRLYSLEUASP
26   GLUILELYSASNALALEULYS

Samples:

sample_one: Outer Surface Protein A, [U-99% 13C; U-98% 15N], 1.5 mM; sodium phosphate 10 mM; sodium chloride 50 mM; EDTA 0.05 mM

sample_conditions: ionic strength: 60 mM; pH: 6.0; pressure: 1.0 atm; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

BMRB 4077
PDB
EMBL CAA32579 CAA34487 CAA49314 CAA56467 CAA59729
GB AAA20947 AAA20949 AAA20951 AAA20953 AAA20955
REF NP_045688 WP_010258120 WP_010890378 WP_012615002 WP_012665570
SP B7IZU3 C6C2D6 P0CL66
AlphaFold B7IZU3 C6C2D6 P0CL66

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks