;mlevi4pr3d ;MANUFACTURER: Bruker ;MODEL: DMX ;AUTHOR: Charles G. Hoogstraten ;DESCRIPTION: avance-version ;BMRB Pulse Sequence Accession Number: 1 ;3D sequence with ; homonuclear Hartman-Hahn transfer using MLEV17 sequence ; for mixing ; H-1/X correlation via heteronuclear zero and double quantum ; coherence ; using two power levels for excitation and spinlock ;phase sensitive using TPPI ;with decoupling during acquisition ;with presaturation during relaxation delay ;converted to states-tppi cgh 1/1/95 ;dipsi.wmw converted cgh 1/26/95 ;homonuclear Hartman-Hahn transfer using DIPSI-2 sequence ;Shaka et. al. JMR 77 (1988), 274 ff. Mol. Phys. 68 (1989), 509 ff. ;L1: loop for DIPSI cycle: l1=mixing time/(p5*115.11) #include #include p4=p3*2 ;;d0=3u ;;d2=1s/(cnst2*2) ;;d10=3u ;;d11=30m ;;d12=20u ;;d13=3u 1 d11 ze d11 pl12:f2 2 d11 do:f2 3 d11 6 d11 7 d11 4 4u d1 pl2:f2 p1 ph3 d0 (p4 ph0):f2 d0 10u pl3:f1 p17 ph21 5 p5*3.556 ph11 ;A p5*4.556 ph12 p5*3.222 ph11 p5*3.167 ph12 p5*0.333 ph11 p5*2.722 ph12 p5*4.167 ph11 p5*2.944 ph12 p5*4.111 ph11 p5*3.556 ph12 ;a p5*4.556 ph11 p5*3.222 ph12 p5*3.167 ph11 p5*0.333 ph12 p5*2.722 ph11 p5*4.167 ph12 p5*2.944 ph11 p5*4.111 ph12 p5*3.556 ph12 p5*4.556 ph11 p5*3.222 ph12 p5*3.167 ph11 p5*0.333 ph12 p5*2.722 ph11 p5*4.167 ph12 p5*2.944 ph11 p5*4.111 ph12 p5*3.556 ph11 p5*4.556 ph12 p5*3.222 ph11 p5*3.167 ph12 p5*0.333 ph11 p5*2.722 ph12 p5*4.167 ph11 p5*2.944 ph12 p5*4.111 ph11 lo to 5 times l1 p17*1.5 ph21 d13 GRADIENT(cnst22) d4 pl1:f1 ;d4=2.3 ms - length of grads 2, 5 (p4 ph0):f2 (p2 ph0) GRADIENT(cnst22) d4 p8 ph0 ;p8=1.0ms d13 p1 ph1 GRADIENT(cnst23) d9 ;settling time (p3 ph4):f2 d10 p2 ph0 d10 p3:f2 ph0 GRADIENT(cnst24) ;cnst24<0 d9 p1 ph0 GRADIENT(cnst25) ;cnst25<0 d4 (p2 ph0) (p4 ph0):f2 GRADIENT(cnst25) d4 pl12:f2 p8*1.5 ph0 d13 go=2 ph31 cpd2:f2 d11 do:f2 wr #0 if #0 ip3 zd lo to 3 times 2 d11 id0 ip31*2 lo to 6 times l6 d11 rd0 ip4 lo to 7 times 2 d11 id10 ip31*2 lo to 4 times l4 exit ph0=0 ph1=1 ph2=2 ph3=0 0 2 2 ph4=0 2 ph11= 0 ph12= 2 ph21= 1 ph31=0 2 2 0 ;pl1 : f1 channel - power level for pulse (default) ;pl2 : f2 channel - power level for pulse (default) ;pl9 : f1 channel - power level for presaturation ;pl10: f1 channel - power level for TOCSY-spinlock ;pl12: f2 channel - power level for CPD/BB decoupling ;p1 : f1 channel - 90 degree high power pulse ;p2 : f1 channel - 180 degree high power pulse ;p3 : f2 channel - 90 degree high power pulse ;p4 : f2 channel - 180 degree high power pulse ;p5 : f1 channel - 60 degree low power pulse ;p6 : f1 channel - 90 degree low power pulse ;p7 : f1 channel - 180 degree low power pulse ;p17: f1 channel - trim pulse [2.5 msec] ;p31: - 90 degree pulse for decoupling sequence ;d0 : incremented delay (F1 in 3D) [3 usec] ;d1 : relaxation delay; 1-5 * T1 ;d2 : 1/((2J)XH) ;d10: incremented delay (F2 in 3D) [3 usec] ;d11: delay for disk I/O [30 msec] ;d12: delay for power switching [20 usec] ;d13: short delay [3 usec] ;L1: loop for MLEV cycle: (((p6*64) + p5 ) * l1) + (p17*2) = mixing time ;in0: 1/(4 * SW(H)) = (1/2) DW(H) ;nd0: 4 ;in10: 1/(4 * SW(X)) = (1/2) DW(X) ;nd10: 4 ;NS: 8 * n ;DS: 32 ;td1: number of experiments in F1 ;td2: number of experiments in F2 ;MC2: TPPI in F1 ;MC2: TPPI in F2 ;cpd2: decoupling according to sequence defined by cpdprg2