********  PROTEIN ********

Name:  			At5g39720.1
Organism: 		Arabidopsis thaliana
Gene:			At5g39720.1	
CESG ID:		GO.22446
Expression system:      Wheat germ cell-free system
PDB code:		2g0q
BMRB code:		7007
Sample conditions:	0.9 mM [U-15N/13C]At5g39720.1
			100 mM NaCl
			10 mM BisTris (2H-labeled) buffer
			 5 mM DTT
			pH 7.0, 298 K

******** NMR EXPERIMENTS ********

Spectrometer:		Bruker DRX 600 MHz

HSQC:			15N HSQC, 13C aromatic HSQC
Triple resonance:	HNCA, HNCO, HNCOCA, HNCACB, HNCACO, CCONH, HCCH-TOCSY, HBHACONH, HCCONH
NOESY:			3D 15N-NOESY, 3D 13C-NOESY aliphatic, 3D 13C-NOESY aromatic

--Each directory contains raw data, pulse sequences, acquisition parameters, and processing scripts

******** SOFTWARE ********

Collection:		XWINNMR
Processing:		NMRPipe
Analysis:		XEASY
Peak picking:		SPSCAN and XEASY
Backbone assignments:	GARANT
Structure calculation:	CYANA (CANDID used for automated NOE assignments)
Water refinement:	XPLOR-NIH

******** CONTACT ********

Brian F. Volkman:	bvolkman@biochem.mcw.edu

Data Directory 	Experiment number	Experiment

030305.5g39720		1					1D
030305.5g39720		4					15N HSQC
030305.5g39720		5					15N NOESY
030305.5g39720		7					13C NOESY aliphatic
030305.5g39720		9					Aromatic HSQC
030305.5g39720	   10					13C NOESY aromatic
030705.5g39720		3					3D HNCO
030705.5g39720		5					3D HNCOCA
030705.5g39720		7					3D HNCA
030705.5g39720		9					3D HNCACB
030705.5g39720	   11					3D HNCACO
031005.5g39720		3					HCCH-TOCSY
031005.5g39720		5					3D HBHACONH
031005.5g39720		7					3D HCCONH
031005.5g39720		9					3D CCONH
031005.5g39720	   11					15N hetNOE
not run								13C CT-HSQC aliphatic
not run								13C CT-HSQC aromatic
[ICO]NameLast modifiedSizeDescription
[PARENTDIR]Parent Directory  -  
[ ]timedomain_data.tar2021-02-13 19:01 887M 
[TXT]README.txt2021-02-13 19:00 1.7K 
[DIR]analysis/2021-02-13 19:02 -  
[DIR]031805.5g39720/2021-02-13 19:00 -  
[DIR]031005.5g39720/2021-02-13 19:00 -  
[DIR]030705.5g39720/2021-02-13 19:00 -  
[DIR]030305.5g39720/2021-02-13 19:00 -  
********  PROTEIN ********

Name:  			At5g39720.1
Organism: 		Arabidopsis thaliana
Gene:			At5g39720.1	
CESG ID:		GO.22446
Expression system:      Wheat germ cell-free system
PDB code:		2g0q
BMRB code:		7007
Sample conditions:	0.9 mM [U-15N/13C]At5g39720.1
			100 mM NaCl
			10 mM BisTris (2H-labeled) buffer
			 5 mM DTT
			pH 7.0, 298 K

******** NMR EXPERIMENTS ********

Spectrometer:		Bruker DRX 600 MHz

HSQC:			15N HSQC, 13C aromatic HSQC
Triple resonance:	HNCA, HNCO, HNCOCA, HNCACB, HNCACO, CCONH, HCCH-TOCSY, HBHACONH, HCCONH
NOESY:			3D 15N-NOESY, 3D 13C-NOESY aliphatic, 3D 13C-NOESY aromatic

--Each directory contains raw data, pulse sequences, acquisition parameters, and processing scripts

******** SOFTWARE ********

Collection:		XWINNMR
Processing:		NMRPipe
Analysis:		XEASY
Peak picking:		SPSCAN and XEASY
Backbone assignments:	GARANT
Structure calculation:	CYANA (CANDID used for automated NOE assignments)
Water refinement:	XPLOR-NIH

******** CONTACT ********

Brian F. Volkman:	bvolkman@biochem.mcw.edu

Data Directory 	Experiment number	Experiment

030305.5g39720		1					1D
030305.5g39720		4					15N HSQC
030305.5g39720		5					15N NOESY
030305.5g39720		7					13C NOESY aliphatic
030305.5g39720		9					Aromatic HSQC
030305.5g39720	   10					13C NOESY aromatic
030705.5g39720		3					3D HNCO
030705.5g39720		5					3D HNCOCA
030705.5g39720		7					3D HNCA
030705.5g39720		9					3D HNCACB
030705.5g39720	   11					3D HNCACO
031005.5g39720		3					HCCH-TOCSY
031005.5g39720		5					3D HBHACONH
031005.5g39720		7					3D HCCONH
031005.5g39720		9					3D CCONH
031005.5g39720	   11					15N hetNOE
not run								13C CT-HSQC aliphatic
not run								13C CT-HSQC aromatic