********  PROTEIN ********

Name:  			BC019267
Organism: 		Human
Gene:			BC019267	
CESG ID:		GO.33810
Expression system:      Wheat germ cell-free system
PDB code:		1ZR9
BMRB code:		6682
Sample conditions:	0.5 mM [U-15N/13C]BC019267
			100 mM NaCl
			10 mM BisTris (2H-labeled) buffer
			pH 6.0, 298 K

******** NMR EXPERIMENTS ********

Spectrometer:		Bruker DRX 600 MHz

HSQC:			15N HSQC, 13C aliphatic CT-HSQC, 13C aromatic CT-HSQC, 13C aromatic HSQC
Triple resonance:	HNCA, HNCO, HNCOCA, HNCACB, HNCACO, CCONH, HCCH-TOCSY, HBHACONH, HCCONH
NOESY:			3D 15N-NOESY, 3D 13C-NOESY aliphatic, 3D 13C-NOESY aromatic

--Each directory contains raw data, pulse sequences, acquisition parameters, and processing scripts

******** SOFTWARE ********

Collection:		XWINNMR
Processing:		NMRPipe
Analysis:		XEASY
Peak picking:		SPSCAN and XEASY
Backbone assignments:	GARANT
Structure calculation:	CYANA (CANDID used for automated NOE assignments)
Water refinement:	XPLOR-NIH

******** CONTACT ********

Brian F. Volkman:	bvolkman@biochem.mcw.edu
[ICO]NameLast modifiedSizeDescription
[PARENTDIR]Parent Directory  -  
[DIR]011705.bc019267/2021-02-13 18:53 -  
[TXT]README.txt2021-02-13 18:54 1.0K 
[DIR]022405.bc019267/2021-02-13 18:54 -  
[DIR]102504.bc019267/2021-02-13 18:54 -  
[DIR]101904.bc019267/2021-02-13 18:54 -  
[DIR]110404.bc019267/2021-02-13 18:54 -  
[DIR]120204.bc019267/2021-02-13 18:54 -  
[DIR]102804.bc019267/2021-02-13 18:54 -  
[DIR]102104.bc019267/2021-02-13 18:54 -  
[DIR]031705.bc019267/2021-02-13 18:54 -  
[DIR]011005.bc019267/2021-02-13 18:54 -  
[ ]bc019267.tar2021-02-13 18:56 935M 
[DIR]analysis/2021-02-13 18:56 -  
[DIR]101404.bc019267/2021-02-13 18:56 -  
********  PROTEIN ********

Name:  			BC019267
Organism: 		Human
Gene:			BC019267	
CESG ID:		GO.33810
Expression system:      Wheat germ cell-free system
PDB code:		1ZR9
BMRB code:		6682
Sample conditions:	0.5 mM [U-15N/13C]BC019267
			100 mM NaCl
			10 mM BisTris (2H-labeled) buffer
			pH 6.0, 298 K

******** NMR EXPERIMENTS ********

Spectrometer:		Bruker DRX 600 MHz

HSQC:			15N HSQC, 13C aliphatic CT-HSQC, 13C aromatic CT-HSQC, 13C aromatic HSQC
Triple resonance:	HNCA, HNCO, HNCOCA, HNCACB, HNCACO, CCONH, HCCH-TOCSY, HBHACONH, HCCONH
NOESY:			3D 15N-NOESY, 3D 13C-NOESY aliphatic, 3D 13C-NOESY aromatic

--Each directory contains raw data, pulse sequences, acquisition parameters, and processing scripts

******** SOFTWARE ********

Collection:		XWINNMR
Processing:		NMRPipe
Analysis:		XEASY
Peak picking:		SPSCAN and XEASY
Backbone assignments:	GARANT
Structure calculation:	CYANA (CANDID used for automated NOE assignments)
Water refinement:	XPLOR-NIH

******** CONTACT ********

Brian F. Volkman:	bvolkman@biochem.mcw.edu