********  PROTEIN ********

Name:  			Thioredoxin h1
Organism: 		Arabidopsis thaliana
Gene:			At3g51030.1
Expression system:      Wheat germ cell-free system
PDB code:		1XFL
BMRB code:		6318
Sample conditions:	0.5 mM [U-15N; U-13C]At3g51030
			50 mM KCl
			10 mM phosphate buffer
			pH 5.5, 298 K

******** NMR EXPERIMENTS ********

Spectrometer:		Bruker DRX 600 MHz

HSQC:			15N HSQC, 13C aliphatic CT-HSQC, 13C aromatic CT-			HSQC, 13C aromatic HSQC
Triple resonance:	HNCA, HNCO, HNCOCA, HNCACO, CCONH, HCCH-TOCSY, 				HCCONH
NOESY:			3D 15N-NOESY, 3D 13C-NOESY aliphatic, 3D 13C-NOESY 			aromatic

--Each directory contains raw data, pulse sequences, acquisition parameters, and processing scripts

******** SOFTWARE ********

Collection:		XWINNMR
Processing:		NMRPipe
Analysis:		XEASY
Peak picking:		SPSCAN and XEASY
Backbone assignments:	GARANT
Structure calculation:	CYANA (CANDID used for automated NOE assignments)
Water refinement:	XPLOR-NIH

******** CONTACT ********

Brian F. Volkman:	bvolkman@biochem.mcw.edu

Directory	Exp#	Type
030804.3g51030	1	1D
030804.3g51030	2	15N HSQC
030804.3g51030	3	HNCO
030804.3g51030	4	15N HSQC
030804.3g51030	5	HNCA
030804.3g51030	6	15N HSQC
030804.3g51030	7	HNCOCA
030804.3g51030	8	15N HSQC
030804.3g51030	9	HNCACB
030804.3g51030	10	15N HSQC
030804.3g51030	11	HNCACO
030804.3g51030	12	15N HSQC
030804.3g51030	13	CCONH
030804.3g51030	14	15N HSQC
030804.3g51030	15	HCCH-Tocsy
030804.3g51030	16	15N HSQC
		
032504.3g51030	1	15N HSQC
032504.3g51030	3	13C CT-HSQC aromatic
032504.3g51030	6	13C CT-HSQC aliphatic
032504.3g51030	7	Aromatic HSQC
032504.3g51030	8	15N HSQC
		
041304.3g51030	1	1D
041304.3g51030	2	15N HSQC
041304.3g51030	3	HCCONH
041304.3g51030	4	13C NOESY aromatic
		
041504.3g51030	1	13C NOESY aliphatic
		
041704.3g51030	1	15N NOESY