# 1 "C:/Bruker/TopSpin3.6.4/exp/stan/nmr/lists/pp/user/15N-HSQCwg.WJH"
;15N-HSQCwg.WJH 
; avance-version
; 2009.9.30
; 2D 1H-15N HSQC with 13C decoupling and Watergate reverse INEPT
;
; phase sensitive using States-TPPI method during t1, nd0=2
;

# 1 "C:/Bruker/TopSpin3.6.4/exp/stan/nmr/lists/pp/Avance.incl" 1
;Avance3.incl
;   for AV III
;
;avance-version (13/06/17)
;
;$CLASS=HighRes Incl
;$COMMENT=

# 169 "C:/Bruker/TopSpin3.6.4/exp/stan/nmr/lists/pp/Avance.incl"
;$Id: Avance3.incl,v 1.10.2.2 2013/08/30 09:43:33 ber Exp $
# 9 "C:/Bruker/TopSpin3.6.4/exp/stan/nmr/lists/pp/user/15N-HSQCwg.WJH" 2

# 1 "C:/Bruker/TopSpin3.6.4/exp/stan/nmr/lists/pp/Grad.incl" 1
;Grad2.incl  -  include file for Gradient Spectroscopy
;   for TCU3
;
;avance-version (07/01/17)
;
;$CLASS=HighRes Incl
;$COMMENT=

# 27 "C:/Bruker/TopSpin3.6.4/exp/stan/nmr/lists/pp/Grad.incl"
define list<gradient> EA=<EA>


# 31 "C:/Bruker/TopSpin3.6.4/exp/stan/nmr/lists/pp/Grad.incl"
;$Id: Grad2.incl,v 1.14 2012/01/31 17:49:21 ber Exp $
# 10 "C:/Bruker/TopSpin3.6.4/exp/stan/nmr/lists/pp/user/15N-HSQCwg.WJH" 2

# 1 "C:/Bruker/TopSpin3.6.4/exp/stan/nmr/lists/pp/Delay.incl" 1
;Delay.incl  -  include file for commonly used delays
;
;version (13/08/07)
;
;$CLASS=HighRes Incl
;$COMMENT=

# 9 "C:/Bruker/TopSpin3.6.4/exp/stan/nmr/lists/pp/Delay.incl"
;general delays

define delay DELTA
define delay DELTA1
define delay DELTA2
define delay DELTA3
define delay DELTA4
define delay DELTA5
define delay DELTA6
define delay DELTA7
define delay DELTA8
define delay DELTA9
define delay DELTA10
define delay DELTA11
define delay DELTA12
define delay DELTA13
define delay DELTA14
define delay DELTA15
define delay DELTA16

define delay TAU
define delay TAU1
define delay TAU2
define delay TAU3
define delay TAU4
define delay TAU5
define delay TAU6
define delay TAU7
define delay TAU8
define delay TAU9
# 40 "C:/Bruker/TopSpin3.6.4/exp/stan/nmr/lists/pp/Delay.incl"
define delay INCR1
define delay INCR2
define delay INCR3
define delay INCR4
define delay INCR5
define delay INCR6


;delays for centering pulses
# 50 "C:/Bruker/TopSpin3.6.4/exp/stan/nmr/lists/pp/Delay.incl"
define delay CEN_HN1
define delay CEN_HN2
define delay CEN_HN3
define delay CEN_HC1
define delay CEN_HC2
define delay CEN_HC3
define delay CEN_HC4
define delay CEN_HP1
define delay CEN_HP2
define delay CEN_CN1
define delay CEN_CN2
define delay CEN_CN3
define delay CEN_CN4
define delay CEN_CP1
define delay CEN_CP2


;loop counters
# 69 "C:/Bruker/TopSpin3.6.4/exp/stan/nmr/lists/pp/Delay.incl"
define loopcounter COUNTER
define loopcounter SCALEF
define loopcounter FACTOR1
define loopcounter FACTOR2
define loopcounter FACTOR3
define loopcounter FACTOR4
define loopcounter FACTOR5
define loopcounter FACTOR6


# 80 "C:/Bruker/TopSpin3.6.4/exp/stan/nmr/lists/pp/Delay.incl"
;$Id: Delay.incl,v 1.14.2.1 2013/08/30 09:43:33 ber Exp $
# 11 "C:/Bruker/TopSpin3.6.4/exp/stan/nmr/lists/pp/user/15N-HSQCwg.WJH" 2


;
;
;
;
;
;
# 21 "C:/Bruker/TopSpin3.6.4/exp/stan/nmr/lists/pp/user/15N-HSQCwg.WJH"
"in0=inf1/2"

"p2=p1*2"
"p22=p21*2"

"d26=2.45m"
"d27=2.65m"
# 30 "C:/Bruker/TopSpin3.6.4/exp/stan/nmr/lists/pp/user/15N-HSQCwg.WJH"
    "d0=((cnst0*2+1)*in0-p9-3u-p7)/2"


# 35 "C:/Bruker/TopSpin3.6.4/exp/stan/nmr/lists/pp/user/15N-HSQCwg.WJH"
"DELTA2=d27-p16-d16-p11-12u"

"CEN_HN2=(p22-p2)/2"
"CEN_HC2=(p9+3u+p7-p2)/2"

"l3=(td1/2)"
# 1 "mc_line 43 file C:/Bruker/TopSpin3.6.4/exp/stan/nmr/lists/pp/user/15N-HSQCwg.WJH dc-measurement inserted automatically"

    dccorr
# 43 "C:/Bruker/TopSpin3.6.4/exp/stan/nmr/lists/pp/user/15N-HSQCwg.WJH"
# 43 "C:/Bruker/TopSpin3.6.4/exp/stan/nmr/lists/pp/user/15N-HSQCwg.WJH"
1 ze
2 d11 do:f3
3 3m
4 d1
  40u pl1:f1 pl20:f2 pl3:f3
  40u setnmr3|0 setnmr0|34|32|33 ctrlgrad 0
;-------------------- destroy 15N  ---------------------
  (p21 ph0):f3
  d13
  p16:gp1
  d16
;--------------------- INEPT to N --------------------------
  (p1 ph1):f1 
  d26
  (CEN_HN2 p2 ph1):f1 (p22 ph1):f3
  d26
  (p1 ph2):f1 
  4u pl0:f1
;  (p11:sp1 ph1:r):f1
  d13
  p16:gp2
  d16 pl1:f1
;d25
  (p21 ph3):f3
;-------------------- 15N evolution (t1) ------------
  d0

    (CEN_HC2 p2 ph4):f1 (p9:sp9 ph4 3u p7:sp7 ph4):f2

# 74 "C:/Bruker/TopSpin3.6.4/exp/stan/nmr/lists/pp/user/15N-HSQCwg.WJH"
  d0
;--------------------- back to 1H --------------------------
  (p21 ph5):f3
  d13
  p16:gp3
  d16 pl0:f1
  (p11:sp1 ph6):f1
  4u
  4u pl1:f1

  (p1 ph1) 
  4u
  p16:gp4
  d16 pl0:f1
  DELTA2
  (p11:sp1 ph6):f1
  4u
  4u pl1:f1
  (CEN_HN2 p2 ph1):f1 (p22 ph1):f3
  4u pl0:f1
  (p11:sp1 ph6):f1
  4u
  DELTA2
  p16:gp4
  d16 pl16:f3
  4u setnmr3^0 setnmr0^34^32^33 ctrlgrad 7
  go=2 ph31 cpd3:f3 
  d11 do:f3 wr #0 if #0 ip3 zd 
  lo to 3 times 2
  3m id0
  lo to 4 times l3
exit

ph0=0
ph1=0
ph2=1 1 3 3
ph3=0
ph4=0 0 0 0 2 2 2 2
ph5=0 2
ph6=2
ph31=0 2 2 0
# 117 "C:/Bruker/TopSpin3.6.4/exp/stan/nmr/lists/pp/user/15N-HSQCwg.WJH"
;pl0 : 120dB
;pl1 : f1 channel - power level for pulse (default)
;pl3 : f3 channel - power level for pulse (default)
;pl16: f3 channel - power level for CPD/BB decoupling
;sp1: f1 channel - shaped pulse  90 degree  (H2O on resonance)
;sp7: f2 channel - shaped pulse 180 degree  (Ca on resonance)
;sp9: f2 channel - shaped pulse 180 degree  (C=O on resonance)
;p1 : f1 channel -  90 degree high power pulse
;p2 : f1 channel - 180 degree high power pulse
;p7: Ca 180, 58.36us(spnam7=square256.wjh) at power sp7(ca. 5.2db),118ppm
;   offset Ca-115 (-59ppm,-7420Hz)
;p9: CO 180, 96.8us(spnam9=sinc256.wjh) at power sp9(ca. 6.2db),118ppm
;   offset CO-115 (59ppm,7420Hz)
;p16: homospoil/gradient pulse                         [1 msec]
;p21: f3 channel -  90 degree high power pulse
;p22: f3 channel - 180 degree high power pulse
;d0 : incremented delay (F1 in 3D)                     [3 usec]
;d1 : relaxation delay; 1-5 * T1
;d11: delay for disk I/O                               [30 msec]
;d13: short delay                                      [4 usec]
;d16: delay for homospoil/gradient recovery
;d26: 1/(4J(NH)                                        [2.75 msec]
;nd0: 2
;NS: 4 * n
;DS: >= 16
;td1: number of experiments in F1
;FnMODE: States-TPPI (or TPPI) in F1
;cpd3: decoupling according to sequence defined by cpdprg3
;pcpd3: f3 channel - 90 degree pulse for decoupling sequence

;for z-only gradients:
;gpz1: 50%
;gpz2: 40%
;gpz3: 25%
;gpz4: 70%

;use gradient files:   
;gpnam1: SINE.100
;gpnam2: SINE.100
;gpnam3: SINE.100
;gpnam4: SINE.100
