USER MOD reduce.3.24.130724 H: found=0, std=0, add=64, rem=0, adj=8 USER MOD reduce.3.24.130724 removed 64 hydrogens (64 hets) HEADER ANTIBIOTIC 18-JUN-03 1UGT TITLE STRUCTURAL STUDIES OF CU(I)-BLEOMYCIN COMPND MOL_ID: 1; COMPND 2 MOLECULE: BLEOMYCIN A2; COMPND 3 CHAIN: A; COMPND 4 ENGINEERED: YES SOURCE MOL_ID: 1; SOURCE 2 SYNTHETIC: YES KEYWDS METAL COMPLEX, ANTIBIOTIC EXPDTA SOLUTION NMR MDLTYP MINIMIZED AVERAGE AUTHOR T.E.LEHMANN REVDAT 2 24-FEB-09 1UGT 1 VERSN REVDAT 1 27-APR-04 1UGT 0 JRNL AUTH T.E.LEHMANN JRNL TITL STRUCTURAL STUDY OF COPPER(I)-BLEOMYCIN. JRNL REF J.BIOL.INORG.CHEM. V. 9 323 2004 JRNL REFN ISSN 0949-8257 JRNL PMID 15015041 JRNL DOI 10.1007/S00775-004-0530-7 REMARK 1 REMARK 2 REMARK 2 RESOLUTION. NOT APPLICABLE. REMARK 3 REMARK 3 REFINEMENT. REMARK 3 PROGRAM : DISCOVER 2003 REMARK 3 AUTHORS : ACCELRYS REMARK 3 REMARK 3 OTHER REFINEMENT REMARKS: THE STRUCTURE IS BASED ON A TOTAL OF REMARK 3 40 NON-TRIVIAL NOE-DERIVED DISTANCE CONSTRAINTS REMARK 4 REMARK 4 1UGT COMPLIES WITH FORMAT V. 3.15, 01-DEC-08 REMARK 100 REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 26-JUN-03. REMARK 100 THE RCSB ID CODE IS RCSB005807. REMARK 210 REMARK 210 EXPERIMENTAL DETAILS REMARK 210 EXPERIMENT TYPE : NMR REMARK 210 TEMPERATURE (KELVIN) : 278 REMARK 210 PH : 8.1 REMARK 210 IONIC STRENGTH : 5MM REMARK 210 PRESSURE : AMBIENT REMARK 210 SAMPLE CONTENTS : 5MM CU(I)BLM 1H, 90% H2O, 10% REMARK 210 D2O; 5MM CU(I)BLM, 100% D2O REMARK 210 REMARK 210 NMR EXPERIMENTS CONDUCTED : 2D NOESY REMARK 210 SPECTROMETER FIELD STRENGTH : 500 MHZ REMARK 210 SPECTROMETER MODEL : DRX REMARK 210 SPECTROMETER MANUFACTURER : BRUKER REMARK 210 REMARK 210 STRUCTURE DETERMINATION. REMARK 210 SOFTWARE USED : XWINNMR 2.5, NMRPIPE 2002, REMARK 210 DISCOVER 2003 REMARK 210 METHOD USED : MOLECULAR DYNAMICS REMARK 210 REMARK 210 CONFORMERS, NUMBER CALCULATED : 10 REMARK 210 CONFORMERS, NUMBER SUBMITTED : 1 REMARK 210 CONFORMERS, SELECTION CRITERIA : STRUCTURES WITH THE LOWEST REMARK 210 ENERGY REMARK 210 REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1 REMARK 210 REMARK 210 REMARK: THIS STRUCTURE WAS DETERMINED USING STANDARD 2D REMARK 210 HOMONUCEAR TECHNIQUES. REMARK 215 REMARK 215 NMR STUDY REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION REMARK 215 NMR DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE THAT REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON REMARK 215 THESE RECORDS ARE MEANINGLESS. REMARK 610 REMARK 610 MISSING HETEROATOM REMARK 610 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER; REMARK 610 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER; REMARK 610 I=INSERTION CODE): REMARK 610 M RES C SSEQI REMARK 610 BLM A 1 REMARK 620 REMARK 620 METAL COORDINATION REMARK 620 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER; REMARK 620 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE): REMARK 620 REMARK 620 COORDINATION ANGLES FOR: M RES CSSEQI METAL REMARK 620 CO A 10 CO REMARK 620 N RES CSSEQI ATOM REMARK 620 1 BLM A 1 NC REMARK 620 2 BLM A 1 NB 77.7 REMARK 620 3 BLM A 1 NG 82.3 121.3 REMARK 620 4 BLM A 1 NJ 113.8 114.4 124.2 REMARK 620 N 1 2 3 REMARK 800 REMARK 800 SITE REMARK 800 SITE_IDENTIFIER: AC1 REMARK 800 EVIDENCE_CODE: SOFTWARE REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE BLM A 1 REMARK 800 SITE_IDENTIFIER: AC2 REMARK 800 EVIDENCE_CODE: SOFTWARE REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE CO A 10 HET BLM A 1 139 HET CO A 10 1 HETNAM BLM BLEOMYCIN A2 HETNAM CO COBALT (II) ION HETSYN BLM N1-[3-(DIMETHYLSULFONIO)-PROPYL]BLEOMYCINAMIDE FORMUL 1 BLM C55 H85 N17 O21 S3 FORMUL 2 CO CO 2+ LINK CO CO A 10 NC BLM A 1 1555 1555 2.01 LINK CO CO A 10 NB BLM A 1 1555 1555 2.02 LINK CO CO A 10 NG BLM A 1 1555 1555 2.03 LINK CO CO A 10 NJ BLM A 1 1555 1555 2.03 SITE *** AC1 1 CO A 10 SITE *** AC2 1 BLM A 1 CRYST1 1.000 1.000 1.000 90.00 90.00 90.00 P 1 1 ORIGX1 1.000000 0.000000 0.000000 0.00000 ORIGX2 0.000000 1.000000 0.000000 0.00000 ORIGX3 0.000000 0.000000 1.000000 0.00000 SCALE1 1.000000 0.000000 0.000000 0.00000 SCALE2 0.000000 1.000000 0.000000 0.00000 SCALE3 0.000000 0.000000 1.000000 0.00000 USER MOD ----------------------------------------------------------------- USER MOD scores for adjustable sidechains, with "set" totals for H,N and Q USER MOD "o" means original, "f" means flipped, "180deg" is methyl default USER MOD "!" flags a clash with an overlap of 0.40A or greater USER MOD flip categories: "K"=keep, "C"=clashes, "X"=uncertain, "F"=flip USER MOD Single : A 1 BLM O58 : rot -30:sc= 0.144 USER MOD Single : A 1 BLM O59 : rot 180:sc= 0 USER MOD Single : A 1 BLM O61 : rot 180:sc= 0 USER MOD Single : A 1 BLM O66 : rot 180:sc= 0 USER MOD Single : A 1 BLM O67 : rot 160:sc= -1.23 USER MOD Single : A 1 BLM O69 : rot 180:sc= -0.0234 USER MOD Single : A 1 BLM OH2 : rot 150:sc= 0 USER MOD Single : A 1 BLM OH3 : rot 180:sc= -0.0207 USER MOD ----------------------------------------------------------------- HETATM 1 NA BLM A 1 -14.575 27.241 3.310 1.00 0.00 N HETATM 2 C2 BLM A 1 -13.444 28.824 4.723 1.00 0.00 C HETATM 3 C1 BLM A 1 -13.593 28.128 3.387 1.00 0.00 C HETATM 4 O1 BLM A 1 -12.872 28.395 2.437 1.00 0.00 O HETATM 5 NC BLM A 1 -14.299 31.063 5.490 1.00 0.00 N HETATM 6 C3 BLM A 1 -14.661 29.704 5.003 1.00 0.00 C HETATM 7 NB BLM A 1 -12.253 29.696 4.908 1.00 0.00 N HETATM 8 ND BLM A 1 -17.324 32.877 7.083 1.00 0.00 N HETATM 9 C5 BLM A 1 -15.337 33.392 5.829 1.00 0.00 C HETATM 10 C4 BLM A 1 -16.148 33.472 7.084 1.00 0.00 C HETATM 11 O4 BLM A 1 -15.765 34.104 8.049 1.00 0.00 O HETATM 12 C8 BLM A 1 -15.787 32.472 1.347 1.00 0.00 C HETATM 13 C9 BLM A 1 -14.517 32.756 0.987 1.00 0.00 C HETATM 14 C10 BLM A 1 -13.466 32.749 2.050 1.00 0.00 C HETATM 15 NG BLM A 1 -13.811 32.376 3.231 1.00 0.00 N HETATM 16 C7 BLM A 1 -15.206 32.193 3.579 1.00 0.00 C HETATM 17 NE BLM A 1 -16.157 32.217 2.724 1.00 0.00 N HETATM 18 C6 BLM A 1 -15.329 32.038 5.087 1.00 0.00 C HETATM 19 NF BLM A 1 -16.890 32.395 0.486 1.00 0.00 N HETATM 20 CA BLM A 1 -14.104 33.056 -0.428 1.00 0.00 C HETATM 21 C12 BLM A 1 -12.061 33.186 1.764 1.00 0.00 C HETATM 22 O12 BLM A 1 -11.422 32.932 0.751 1.00 0.00 O HETATM 23 NH BLM A 1 -11.555 33.861 2.806 1.00 0.00 N HETATM 24 C13 BLM A 1 -10.205 34.416 3.008 1.00 0.00 C HETATM 25 C30 BLM A 1 -8.906 33.790 2.597 1.00 0.00 C HETATM 26 O30 BLM A 1 -7.992 34.440 2.107 1.00 0.00 O HETATM 27 C14 BLM A 1 -10.155 34.907 4.459 1.00 0.00 C HETATM 28 C27 BLM A 1 -10.516 33.928 5.553 1.00 0.00 C HETATM 29 OH1 BLM A 1 -10.845 36.116 4.713 1.00 0.00 O HETATM 30 NJ BLM A 1 -11.452 32.855 5.887 1.00 0.00 N HETATM 31 C28 BLM A 1 -9.858 34.147 6.661 1.00 0.00 C HETATM 32 C29 BLM A 1 -11.389 32.705 7.162 1.00 0.00 C HETATM 33 NI BLM A 1 -10.350 33.417 7.710 1.00 0.00 N HETATM 34 NK BLM A 1 -8.868 32.516 2.925 1.00 0.00 N HETATM 35 C34 BLM A 1 -8.488 30.434 5.137 1.00 0.00 C HETATM 36 C36 BLM A 1 -9.004 29.085 5.582 1.00 0.00 C HETATM 37 O36 BLM A 1 -9.856 28.485 4.933 1.00 0.00 O HETATM 38 OH2 BLM A 1 -7.056 29.347 3.524 1.00 0.00 O HETATM 39 C31 BLM A 1 -7.647 31.694 3.012 1.00 0.00 C HETATM 40 CB BLM A 1 -6.898 31.571 1.671 1.00 0.00 C HETATM 41 C33 BLM A 1 -8.054 30.351 3.654 1.00 0.00 C HETATM 42 CC BLM A 1 -7.470 31.183 6.024 1.00 0.00 C HETATM 43 NL BLM A 1 -8.485 28.559 6.822 1.00 0.00 N HETATM 44 C37 BLM A 1 -9.414 28.423 7.974 1.00 0.00 C HETATM 45 C40 BLM A 1 -10.841 28.010 7.671 1.00 0.00 C HETATM 46 O40 BLM A 1 -11.727 28.827 7.462 1.00 0.00 O HETATM 47 C38 BLM A 1 -9.327 29.728 8.774 1.00 0.00 C HETATM 48 OH3 BLM A 1 -7.971 29.987 9.112 1.00 0.00 O HETATM 49 CD BLM A 1 -10.137 29.657 10.059 1.00 0.00 C HETATM 50 NM BLM A 1 -11.067 26.707 7.639 1.00 0.00 N HETATM 51 O59 BLM A 1 -9.660 39.672 7.668 1.00 0.00 O HETATM 52 O58 BLM A 1 -12.653 37.742 6.885 1.00 0.00 O HETATM 53 C61 BLM A 1 -8.600 36.884 8.485 1.00 0.00 C HETATM 54 O61 BLM A 1 -8.792 37.528 9.744 1.00 0.00 O HETATM 55 O56 BLM A 1 -12.060 38.337 4.367 1.00 0.00 O HETATM 56 C60 BLM A 1 -9.707 37.176 7.453 1.00 0.00 C HETATM 57 O62 BLM A 1 -9.151 37.058 6.154 1.00 0.00 O HETATM 58 C63 BLM A 1 -10.054 37.276 5.080 1.00 0.00 C HETATM 59 C57 BLM A 1 -10.966 38.487 5.236 1.00 0.00 C HETATM 60 C58 BLM A 1 -11.586 38.655 6.597 1.00 0.00 C HETATM 61 C59 BLM A 1 -10.458 38.499 7.582 1.00 0.00 C HETATM 62 C69 BLM A 1 -13.225 38.551 2.434 1.00 0.00 C HETATM 63 C68 BLM A 1 -12.758 37.130 2.004 1.00 0.00 C HETATM 64 C67 BLM A 1 -11.547 37.338 1.063 1.00 0.00 C HETATM 65 C65 BLM A 1 -10.442 37.936 1.945 1.00 0.00 C HETATM 66 O64 BLM A 1 -10.826 39.183 2.563 1.00 0.00 O HETATM 67 C64 BLM A 1 -12.091 39.177 3.257 1.00 0.00 C HETATM 68 O68 BLM A 1 -13.847 36.403 1.349 1.00 0.00 O HETATM 69 O67 BLM A 1 -11.136 36.108 0.468 1.00 0.00 O HETATM 70 O69 BLM A 1 -13.586 39.346 1.310 1.00 0.00 O HETATM 71 NQ BLM A 1 -15.900 35.591 1.464 1.00 0.00 N HETATM 72 C70 BLM A 1 -14.830 35.959 2.125 1.00 0.00 C HETATM 73 O70 BLM A 1 -14.752 35.883 3.343 1.00 0.00 O HETATM 74 O66 BLM A 1 -8.513 39.001 0.779 1.00 0.00 O HETATM 75 C66 BLM A 1 -8.972 37.859 1.496 1.00 0.00 C HETATM 0 HO69 BLM A 1 -13.876 40.231 1.615 1.00 0.00 H new HETATM 0 HO67 BLM A 1 -10.608 36.294 -0.337 1.00 0.00 H new HETATM 0 HO66 BLM A 1 -7.573 38.876 0.531 1.00 0.00 H new HETATM 0 HO61 BLM A 1 -8.054 37.296 10.346 1.00 0.00 H new HETATM 0 HO59 BLM A 1 -10.241 40.458 7.746 1.00 0.00 H new HETATM 0 HO58 BLM A 1 -13.117 37.509 6.054 1.00 0.00 H new HETATM 0 HNQ2 BLM A 1 -16.713 35.235 1.967 1.00 0.00 H new HETATM 0 HNQ1 BLM A 1 -15.920 35.661 0.447 1.00 0.00 H new HETATM 0 H662 BLM A 1 -8.343 37.721 2.376 1.00 0.00 H new HETATM 0 H661 BLM A 1 -8.842 36.976 0.870 1.00 0.00 H new HETATM 0 H612 BLM A 1 -7.641 37.197 8.072 1.00 0.00 H new HETATM 0 H611 BLM A 1 -8.542 35.807 8.645 1.00 0.00 H new HETATM 0 HO3 BLM A 1 -7.915 30.822 9.622 1.00 0.00 H new HETATM 0 HO2 BLM A 1 -7.110 28.946 2.631 1.00 0.00 H new HETATM 0 HNL BLM A 1 -7.505 28.287 6.905 1.00 0.00 H new HETATM 0 HNK BLM A 1 -9.756 32.060 3.136 1.00 0.00 H new HETATM 0 HNI BLM A 1 -10.021 33.405 8.675 1.00 0.00 H new HETATM 0 HNH BLM A 1 -12.204 34.014 3.578 1.00 0.00 H new HETATM 0 HNC BLM A 1 -13.460 31.287 6.025 1.00 0.00 H new HETATM 0 HF2 BLM A 1 -16.770 32.561 -0.513 1.00 0.00 H new HETATM 0 HF1 BLM A 1 -17.813 32.173 0.858 1.00 0.00 H new HETATM 0 HDC BLM A 1 -9.759 28.847 10.683 1.00 0.00 H new HETATM 0 HDB BLM A 1 -10.049 30.601 10.597 1.00 0.00 H new HETATM 0 HDA BLM A 1 -11.184 29.472 9.819 1.00 0.00 H new HETATM 0 HD2 BLM A 1 -17.647 32.388 6.248 1.00 0.00 H new HETATM 0 HD1 BLM A 1 -17.911 32.906 7.917 1.00 0.00 H new HETATM 0 HCC BLM A 1 -6.573 30.575 6.140 1.00 0.00 H new HETATM 0 HCB BLM A 1 -7.208 32.131 5.555 1.00 0.00 H new HETATM 0 HCA BLM A 1 -7.910 31.372 7.003 1.00 0.00 H new HETATM 0 HBC BLM A 1 -7.551 31.110 0.930 1.00 0.00 H new HETATM 0 HBB BLM A 1 -6.602 32.562 1.327 1.00 0.00 H new HETATM 0 HBA BLM A 1 -6.010 30.954 1.806 1.00 0.00 H new HETATM 0 HB2 BLM A 1 -12.111 30.185 5.792 1.00 0.00 H new HETATM 0 HB1 BLM A 1 -11.578 29.805 4.151 1.00 0.00 H new HETATM 0 HAC BLM A 1 -14.336 32.201 -1.064 1.00 0.00 H new HETATM 0 HAB BLM A 1 -14.644 33.932 -0.787 1.00 0.00 H new HETATM 0 HAA BLM A 1 -13.032 33.252 -0.460 1.00 0.00 H new HETATM 0 HA2 BLM A 1 -15.170 27.067 4.120 1.00 0.00 H new HETATM 0 HA1 BLM A 1 -14.736 26.732 2.441 1.00 0.00 H new HETATM 0 H69 BLM A 1 -14.127 38.490 3.043 1.00 0.00 H new HETATM 0 H68 BLM A 1 -12.470 36.523 2.862 1.00 0.00 H new HETATM 0 H67 BLM A 1 -11.792 37.998 0.231 1.00 0.00 H new HETATM 0 H65 BLM A 1 -10.387 37.179 2.727 1.00 0.00 H new HETATM 0 H64 BLM A 1 -12.258 40.229 3.487 1.00 0.00 H new HETATM 0 H63 BLM A 1 -9.368 37.490 4.261 1.00 0.00 H new HETATM 0 H60 BLM A 1 -10.480 36.434 7.655 1.00 0.00 H new HETATM 0 H6 BLM A 1 -16.299 31.640 5.385 1.00 0.00 H new HETATM 0 H5X BLM A 1 -15.702 34.154 5.140 1.00 0.00 H new HETATM 0 H5E BLM A 1 -14.307 33.651 6.074 1.00 0.00 H new HETATM 0 H59 BLM A 1 -10.902 38.408 8.573 1.00 0.00 H new HETATM 0 H58 BLM A 1 -12.064 39.633 6.653 1.00 0.00 H new HETATM 0 H57 BLM A 1 -10.323 39.344 5.037 1.00 0.00 H new HETATM 0 H3X BLM A 1 -15.252 29.797 4.092 1.00 0.00 H new HETATM 0 H3E BLM A 1 -15.293 29.215 5.745 1.00 0.00 H new HETATM 0 H38 BLM A 1 -9.733 30.524 8.150 1.00 0.00 H new HETATM 0 H37 BLM A 1 -9.081 27.563 8.555 1.00 0.00 H new HETATM 0 H34 BLM A 1 -9.345 31.095 5.265 1.00 0.00 H new HETATM 0 H33 BLM A 1 -8.934 30.071 3.075 1.00 0.00 H new HETATM 0 H31 BLM A 1 -6.902 32.177 3.644 1.00 0.00 H new HETATM 0 H29 BLM A 1 -12.077 32.087 7.739 1.00 0.00 H new HETATM 0 H28 BLM A 1 -9.014 34.832 6.741 1.00 0.00 H new HETATM 0 H2 BLM A 1 -13.331 27.985 5.410 1.00 0.00 H new HETATM 0 H14 BLM A 1 -9.079 35.068 4.521 1.00 0.00 H new HETATM 0 H13 BLM A 1 -10.179 35.138 2.192 1.00 0.00 H new HETATM 140 CO CO A 10 -12.567 31.675 4.668 1.00 0.00 CO CONECT 1 3 76 77 CONECT 2 3 6 7 78 CONECT 3 1 2 4 CONECT 4 3 CONECT 5 6 18 79 140 CONECT 6 2 5 80 81 CONECT 7 2 82 83 140 CONECT 8 10 84 85 CONECT 9 10 18 86 87 CONECT 10 8 9 11 CONECT 11 10 CONECT 12 13 17 19 CONECT 13 12 14 20 CONECT 14 13 15 21 CONECT 15 14 16 140 CONECT 16 15 17 18 CONECT 17 12 16 CONECT 18 5 9 16 88 CONECT 19 12 89 90 CONECT 20 13 91 92 93 CONECT 21 14 22 23 CONECT 22 21 CONECT 23 21 24 CONECT 24 23 25 27 94 CONECT 25 24 26 34 CONECT 26 25 CONECT 27 24 28 29 95 CONECT 28 27 30 31 CONECT 29 27 58 CONECT 30 28 32 140 CONECT 31 28 33 96 CONECT 32 30 33 97 CONECT 33 31 32 98 CONECT 34 25 39 99 CONECT 35 36 41 42 100 CONECT 36 35 37 43 CONECT 37 36 CONECT 38 41 101 CONECT 39 34 40 41 102 CONECT 40 39 103 104 105 CONECT 41 35 38 39 106 CONECT 42 35 107 108 109 CONECT 43 36 44 110 CONECT 44 43 45 47 111 CONECT 45 44 46 50 CONECT 46 45 CONECT 47 44 48 49 112 CONECT 48 47 113 CONECT 49 47 114 115 116 CONECT 50 45 117 CONECT 51 61 118 CONECT 52 60 119 CONECT 53 54 56 120 121 CONECT 54 53 122 CONECT 55 59 67 CONECT 56 53 57 61 123 CONECT 57 56 58 CONECT 58 29 57 59 124 CONECT 59 55 58 60 125 CONECT 60 52 59 61 126 CONECT 61 51 56 60 127 CONECT 62 63 67 70 128 CONECT 63 62 64 68 129 CONECT 64 63 65 69 130 CONECT 65 64 66 75 131 CONECT 66 65 67 CONECT 67 55 62 66 132 CONECT 68 63 72 CONECT 69 64 133 CONECT 70 62 134 CONECT 71 72 135 136 CONECT 72 68 71 73 CONECT 73 72 CONECT 74 75 137 CONECT 75 65 74 138 139 CONECT 76 1 CONECT 77 1 CONECT 78 2 CONECT 79 5 CONECT 80 6 CONECT 81 6 CONECT 82 7 CONECT 83 7 CONECT 84 8 CONECT 85 8 CONECT 86 9 CONECT 87 9 CONECT 88 18 CONECT 89 19 CONECT 90 19 CONECT 91 20 CONECT 92 20 CONECT 93 20 CONECT 94 24 CONECT 95 27 CONECT 96 31 CONECT 97 32 CONECT 98 33 CONECT 99 34 CONECT 100 35 CONECT 101 38 CONECT 102 39 CONECT 103 40 CONECT 104 40 CONECT 105 40 CONECT 106 41 CONECT 107 42 CONECT 108 42 CONECT 109 42 CONECT 110 43 CONECT 111 44 CONECT 112 47 CONECT 113 48 CONECT 114 49 CONECT 115 49 CONECT 116 49 CONECT 117 50 CONECT 118 51 CONECT 119 52 CONECT 120 53 CONECT 121 53 CONECT 122 54 CONECT 123 56 CONECT 124 58 CONECT 125 59 CONECT 126 60 CONECT 127 61 CONECT 128 62 CONECT 129 63 CONECT 130 64 CONECT 131 65 CONECT 132 67 CONECT 133 69 CONECT 134 70 CONECT 135 71 CONECT 136 71 CONECT 137 74 CONECT 138 75 CONECT 139 75 CONECT 140 5 7 15 30 END