USER MOD reduce.3.24.130724 H: found=0, std=0, add=139, rem=0, adj=2 USER MOD reduce.3.24.130724 removed 137 hydrogens (0 hets) USER MOD ----------------------------------------------------------------- USER MOD scores for adjustable sidechains, with "set" totals for H,N and Q USER MOD "o" means original, "f" means flipped, "180deg" is methyl default USER MOD "!" flags a clash with an overlap of 0.40A or greater USER MOD flip categories: "K"=keep, "C"=clashes, "X"=uncertain, "F"=flip USER MOD Single : A 1 PHE N :NH3+ -140:sc= 0.288 (180deg=0.00591) USER MOD Single : A 4 LYS NZ :NH3+ -170:sc= 0 (180deg=-0.0397) USER MOD Single : A 8 LYS NZ :NH3+ 180:sc= 0 (180deg=0) USER MOD ----------------------------------------------------------------- ATOM 1 N PHE A 1 1.081 -0.408 -0.100 1.00 35.25 N ATOM 2 CA PHE A 1 1.897 -0.288 -1.302 1.00 73.40 C ATOM 3 C PHE A 1 3.323 0.125 -0.952 1.00 42.12 C ATOM 4 O PHE A 1 3.616 0.483 0.190 1.00 72.14 O ATOM 5 CB PHE A 1 1.280 0.730 -2.263 1.00 72.34 C ATOM 6 CG PHE A 1 1.285 2.135 -1.733 1.00 24.11 C ATOM 7 CD1 PHE A 1 0.162 2.660 -1.114 1.00 45.43 C ATOM 8 CD2 PHE A 1 2.413 2.930 -1.853 1.00 44.43 C ATOM 9 CE1 PHE A 1 0.164 3.953 -0.625 1.00 3.03 C ATOM 10 CE2 PHE A 1 2.421 4.223 -1.365 1.00 43.43 C ATOM 11 CZ PHE A 1 1.295 4.736 -0.751 1.00 61.23 C ATOM 0 H1 PHE A 1 0.459 -1.237 -0.184 1.00 35.25 H new ATOM 0 H2 PHE A 1 1.699 -0.521 0.729 1.00 35.25 H new ATOM 0 H3 PHE A 1 0.502 0.449 0.014 1.00 35.25 H new ATOM 0 HA PHE A 1 1.929 -1.263 -1.788 1.00 73.40 H new ATOM 0 HB2 PHE A 1 1.826 0.704 -3.206 1.00 72.34 H new ATOM 0 HB3 PHE A 1 0.253 0.436 -2.481 1.00 72.34 H new ATOM 0 HD1 PHE A 1 -0.725 2.052 -1.012 1.00 45.43 H new ATOM 0 HD2 PHE A 1 3.296 2.535 -2.333 1.00 44.43 H new ATOM 0 HE1 PHE A 1 -0.718 4.351 -0.145 1.00 3.03 H new ATOM 0 HE2 PHE A 1 3.307 4.832 -1.464 1.00 43.43 H new ATOM 0 HZ PHE A 1 1.299 5.747 -0.370 1.00 61.23 H new ATOM 21 N LEU A 2 4.207 0.073 -1.942 1.00 64.22 N ATOM 22 CA LEU A 2 5.604 0.441 -1.740 1.00 45.25 C ATOM 23 C LEU A 2 6.005 1.585 -2.667 1.00 23.13 C ATOM 24 O LEU A 2 5.380 1.829 -3.699 1.00 20.51 O ATOM 25 CB LEU A 2 6.511 -0.767 -1.982 1.00 31.23 C ATOM 26 CG LEU A 2 6.649 -1.746 -0.815 1.00 44.11 C ATOM 27 CD1 LEU A 2 7.256 -1.052 0.395 1.00 55.02 C ATOM 28 CD2 LEU A 2 5.298 -2.351 -0.464 1.00 22.34 C ATOM 0 H LEU A 2 3.981 -0.220 -2.892 1.00 64.22 H new ATOM 0 HA LEU A 2 5.720 0.774 -0.709 1.00 45.25 H new ATOM 0 HB2 LEU A 2 6.132 -1.313 -2.846 1.00 31.23 H new ATOM 0 HB3 LEU A 2 7.504 -0.404 -2.245 1.00 31.23 H new ATOM 0 HG LEU A 2 7.317 -2.552 -1.119 1.00 44.11 H new ATOM 0 HD11 LEU A 2 7.347 -1.764 1.215 1.00 55.02 H new ATOM 0 HD12 LEU A 2 8.243 -0.668 0.137 1.00 55.02 H new ATOM 0 HD13 LEU A 2 6.614 -0.226 0.701 1.00 55.02 H new ATOM 0 HD21 LEU A 2 5.415 -3.045 0.368 1.00 22.34 H new ATOM 0 HD22 LEU A 2 4.607 -1.558 -0.180 1.00 22.34 H new ATOM 0 HD23 LEU A 2 4.903 -2.885 -1.328 1.00 22.34 H new ATOM 40 N PRO A 3 7.074 2.303 -2.292 1.00 44.12 N ATOM 41 CA PRO A 3 7.585 3.431 -3.077 1.00 54.20 C ATOM 42 C PRO A 3 8.222 2.984 -4.388 1.00 53.13 C ATOM 43 O PRO A 3 8.129 3.675 -5.403 1.00 73.22 O ATOM 44 CB PRO A 3 8.636 4.056 -2.157 1.00 43.32 C ATOM 45 CG PRO A 3 9.076 2.942 -1.272 1.00 61.41 C ATOM 46 CD PRO A 3 7.868 2.069 -1.074 1.00 72.31 C ATOM 0 HA PRO A 3 6.791 4.118 -3.368 1.00 54.20 H new ATOM 0 HB2 PRO A 3 9.471 4.461 -2.728 1.00 43.32 H new ATOM 0 HB3 PRO A 3 8.216 4.879 -1.578 1.00 43.32 H new ATOM 0 HG2 PRO A 3 9.892 2.381 -1.727 1.00 61.41 H new ATOM 0 HG3 PRO A 3 9.443 3.322 -0.319 1.00 61.41 H new ATOM 0 HD2 PRO A 3 8.144 1.020 -0.969 1.00 72.31 H new ATOM 0 HD3 PRO A 3 7.316 2.345 -0.175 1.00 72.31 H new ATOM 54 N LYS A 4 8.869 1.824 -4.361 1.00 1.12 N ATOM 55 CA LYS A 4 9.521 1.283 -5.547 1.00 72.25 C ATOM 56 C LYS A 4 8.509 1.045 -6.663 1.00 74.15 C ATOM 57 O LYS A 4 8.732 1.434 -7.810 1.00 64.22 O ATOM 58 CB LYS A 4 10.239 -0.025 -5.208 1.00 73.43 C ATOM 59 CG LYS A 4 11.445 0.160 -4.305 1.00 25.44 C ATOM 60 CD LYS A 4 12.637 0.710 -5.071 1.00 33.22 C ATOM 61 CE LYS A 4 13.349 -0.383 -5.853 1.00 4.11 C ATOM 62 NZ LYS A 4 14.175 -1.250 -4.968 1.00 32.34 N ATOM 0 H LYS A 4 8.956 1.240 -3.529 1.00 1.12 H new ATOM 0 HA LYS A 4 10.253 2.013 -5.893 1.00 72.25 H new ATOM 0 HB2 LYS A 4 9.534 -0.702 -4.725 1.00 73.43 H new ATOM 0 HB3 LYS A 4 10.559 -0.505 -6.133 1.00 73.43 H new ATOM 0 HG2 LYS A 4 11.190 0.838 -3.491 1.00 25.44 H new ATOM 0 HG3 LYS A 4 11.712 -0.795 -3.852 1.00 25.44 H new ATOM 0 HD2 LYS A 4 12.303 1.490 -5.755 1.00 33.22 H new ATOM 0 HD3 LYS A 4 13.335 1.175 -4.375 1.00 33.22 H new ATOM 0 HE2 LYS A 4 12.613 -0.994 -6.375 1.00 4.11 H new ATOM 0 HE3 LYS A 4 13.985 0.070 -6.614 1.00 4.11 H new ATOM 0 HZ1 LYS A 4 14.773 -1.871 -5.550 1.00 32.34 H new ATOM 0 HZ2 LYS A 4 14.778 -0.655 -4.364 1.00 32.34 H new ATOM 0 HZ3 LYS A 4 13.552 -1.830 -4.371 1.00 32.34 H new ATOM 76 N ILE A 5 7.395 0.406 -6.320 1.00 24.01 N ATOM 77 CA ILE A 5 6.348 0.119 -7.293 1.00 5.42 C ATOM 78 C ILE A 5 5.485 1.350 -7.549 1.00 74.42 C ATOM 79 O ILE A 5 4.953 1.531 -8.645 1.00 42.25 O ATOM 80 CB ILE A 5 5.447 -1.038 -6.825 1.00 60.52 C ATOM 81 CG1 ILE A 5 4.887 -0.747 -5.432 1.00 13.33 C ATOM 82 CG2 ILE A 5 6.223 -2.347 -6.827 1.00 34.32 C ATOM 83 CD1 ILE A 5 3.730 -1.642 -5.046 1.00 53.31 C ATOM 0 H ILE A 5 7.194 0.077 -5.375 1.00 24.01 H new ATOM 0 HA ILE A 5 6.846 -0.172 -8.218 1.00 5.42 H new ATOM 0 HB ILE A 5 4.612 -1.132 -7.519 1.00 60.52 H new ATOM 0 HG12 ILE A 5 5.684 -0.861 -4.698 1.00 13.33 H new ATOM 0 HG13 ILE A 5 4.561 0.292 -5.390 1.00 13.33 H new ATOM 0 HG21 ILE A 5 5.573 -3.156 -6.494 1.00 34.32 H new ATOM 0 HG22 ILE A 5 6.577 -2.558 -7.836 1.00 34.32 H new ATOM 0 HG23 ILE A 5 7.076 -2.266 -6.153 1.00 34.32 H new ATOM 0 HD11 ILE A 5 3.384 -1.379 -4.046 1.00 53.31 H new ATOM 0 HD12 ILE A 5 2.915 -1.511 -5.758 1.00 53.31 H new ATOM 0 HD13 ILE A 5 4.056 -2.682 -5.055 1.00 53.31 H new ATOM 95 N LEU A 6 5.350 2.193 -6.532 1.00 12.30 N ATOM 96 CA LEU A 6 4.552 3.409 -6.647 1.00 13.11 C ATOM 97 C LEU A 6 5.212 4.406 -7.594 1.00 11.34 C ATOM 98 O LEU A 6 4.581 4.902 -8.528 1.00 22.34 O ATOM 99 CB LEU A 6 4.357 4.047 -5.270 1.00 44.13 C ATOM 100 CG LEU A 6 3.933 5.516 -5.265 1.00 53.13 C ATOM 101 CD1 LEU A 6 2.630 5.698 -6.029 1.00 22.21 C ATOM 102 CD2 LEU A 6 3.792 6.026 -3.838 1.00 61.34 C ATOM 0 H LEU A 6 5.783 2.057 -5.618 1.00 12.30 H new ATOM 0 HA LEU A 6 3.579 3.138 -7.056 1.00 13.11 H new ATOM 0 HB2 LEU A 6 3.607 3.471 -4.728 1.00 44.13 H new ATOM 0 HB3 LEU A 6 5.291 3.957 -4.715 1.00 44.13 H new ATOM 0 HG LEU A 6 4.707 6.099 -5.763 1.00 53.13 H new ATOM 0 HD11 LEU A 6 2.344 6.750 -6.015 1.00 22.21 H new ATOM 0 HD12 LEU A 6 2.765 5.372 -7.061 1.00 22.21 H new ATOM 0 HD13 LEU A 6 1.847 5.102 -5.560 1.00 22.21 H new ATOM 0 HD21 LEU A 6 3.490 7.073 -3.854 1.00 61.34 H new ATOM 0 HD22 LEU A 6 3.038 5.439 -3.315 1.00 61.34 H new ATOM 0 HD23 LEU A 6 4.748 5.932 -3.322 1.00 61.34 H new ATOM 114 N ARG A 7 6.486 4.694 -7.347 1.00 14.51 N ATOM 115 CA ARG A 7 7.232 5.632 -8.178 1.00 33.31 C ATOM 116 C ARG A 7 7.092 5.279 -9.656 1.00 34.52 C ATOM 117 O ARG A 7 6.854 6.149 -10.494 1.00 24.13 O ATOM 118 CB ARG A 7 8.709 5.634 -7.781 1.00 21.20 C ATOM 119 CG ARG A 7 9.414 6.950 -8.064 1.00 50.34 C ATOM 120 CD ARG A 7 9.019 8.021 -7.060 1.00 11.33 C ATOM 121 NE ARG A 7 9.758 9.264 -7.265 1.00 60.02 N ATOM 122 CZ ARG A 7 9.865 10.216 -6.345 1.00 65.01 C ATOM 123 NH1 ARG A 7 9.285 10.068 -5.162 1.00 71.13 N ATOM 124 NH2 ARG A 7 10.555 11.319 -6.607 1.00 63.20 N ATOM 0 H ARG A 7 7.023 4.292 -6.579 1.00 14.51 H new ATOM 0 HA ARG A 7 6.819 6.628 -8.019 1.00 33.31 H new ATOM 0 HB2 ARG A 7 8.791 5.409 -6.718 1.00 21.20 H new ATOM 0 HB3 ARG A 7 9.221 4.835 -8.316 1.00 21.20 H new ATOM 0 HG2 ARG A 7 10.493 6.800 -8.032 1.00 50.34 H new ATOM 0 HG3 ARG A 7 9.169 7.286 -9.071 1.00 50.34 H new ATOM 0 HD2 ARG A 7 7.950 8.217 -7.142 1.00 11.33 H new ATOM 0 HD3 ARG A 7 9.200 7.655 -6.049 1.00 11.33 H new ATOM 0 HE ARG A 7 10.217 9.409 -8.164 1.00 60.02 H new ATOM 0 HH11 ARG A 7 8.755 9.221 -4.956 1.00 71.13 H new ATOM 0 HH12 ARG A 7 9.369 10.801 -4.458 1.00 71.13 H new ATOM 0 HH21 ARG A 7 11.004 11.436 -7.516 1.00 63.20 H new ATOM 0 HH22 ARG A 7 10.637 12.050 -5.900 1.00 63.20 H new ATOM 138 N LYS A 8 7.240 3.996 -9.969 1.00 24.11 N ATOM 139 CA LYS A 8 7.130 3.526 -11.345 1.00 20.13 C ATOM 140 C LYS A 8 5.849 4.040 -11.994 1.00 44.23 C ATOM 141 O LYS A 8 5.860 4.483 -13.143 1.00 72.45 O ATOM 142 CB LYS A 8 7.157 1.996 -11.387 1.00 10.34 C ATOM 143 CG LYS A 8 7.721 1.433 -12.679 1.00 43.14 C ATOM 144 CD LYS A 8 7.930 -0.069 -12.589 1.00 74.31 C ATOM 145 CE LYS A 8 9.211 -0.411 -11.843 1.00 55.43 C ATOM 146 NZ LYS A 8 9.626 -1.822 -12.072 1.00 62.20 N ATOM 0 H LYS A 8 7.437 3.263 -9.288 1.00 24.11 H new ATOM 0 HA LYS A 8 7.981 3.914 -11.904 1.00 20.13 H new ATOM 0 HB2 LYS A 8 7.751 1.628 -10.550 1.00 10.34 H new ATOM 0 HB3 LYS A 8 6.144 1.619 -11.248 1.00 10.34 H new ATOM 0 HG2 LYS A 8 7.042 1.658 -13.502 1.00 43.14 H new ATOM 0 HG3 LYS A 8 8.669 1.920 -12.906 1.00 43.14 H new ATOM 0 HD2 LYS A 8 7.080 -0.526 -12.082 1.00 74.31 H new ATOM 0 HD3 LYS A 8 7.968 -0.493 -13.593 1.00 74.31 H new ATOM 0 HE2 LYS A 8 10.008 0.259 -12.165 1.00 55.43 H new ATOM 0 HE3 LYS A 8 9.065 -0.244 -10.776 1.00 55.43 H new ATOM 0 HZ1 LYS A 8 10.503 -2.016 -11.547 1.00 62.20 H new ATOM 0 HZ2 LYS A 8 8.876 -2.462 -11.742 1.00 62.20 H new ATOM 0 HZ3 LYS A 8 9.790 -1.975 -13.088 1.00 62.20 H new ATOM 160 N ILE A 9 4.749 3.980 -11.252 1.00 3.45 N ATOM 161 CA ILE A 9 3.462 4.443 -11.755 1.00 60.21 C ATOM 162 C ILE A 9 3.492 5.939 -12.050 1.00 20.35 C ATOM 163 O ILE A 9 2.967 6.393 -13.066 1.00 51.01 O ATOM 164 CB ILE A 9 2.328 4.151 -10.755 1.00 22.54 C ATOM 165 CG1 ILE A 9 2.325 2.669 -10.373 1.00 31.13 C ATOM 166 CG2 ILE A 9 0.985 4.554 -11.344 1.00 12.32 C ATOM 167 CD1 ILE A 9 2.095 1.743 -11.546 1.00 74.13 C ATOM 0 H ILE A 9 4.723 3.615 -10.300 1.00 3.45 H new ATOM 0 HA ILE A 9 3.270 3.897 -12.679 1.00 60.21 H new ATOM 0 HB ILE A 9 2.498 4.739 -9.853 1.00 22.54 H new ATOM 0 HG12 ILE A 9 3.278 2.421 -9.906 1.00 31.13 H new ATOM 0 HG13 ILE A 9 1.550 2.496 -9.627 1.00 31.13 H new ATOM 0 HG21 ILE A 9 0.194 4.341 -10.625 1.00 12.32 H new ATOM 0 HG22 ILE A 9 0.992 5.620 -11.571 1.00 12.32 H new ATOM 0 HG23 ILE A 9 0.805 3.990 -12.259 1.00 12.32 H new ATOM 0 HD11 ILE A 9 2.106 0.709 -11.201 1.00 74.13 H new ATOM 0 HD12 ILE A 9 1.129 1.964 -12.000 1.00 74.13 H new ATOM 0 HD13 ILE A 9 2.884 1.887 -12.284 1.00 74.13 H new ATOM 179 N VAL A 10 4.112 6.700 -11.154 1.00 11.34 N ATOM 180 CA VAL A 10 4.214 8.145 -11.318 1.00 53.42 C ATOM 181 C VAL A 10 4.929 8.502 -12.616 1.00 61.45 C ATOM 182 O VAL A 10 4.429 9.294 -13.415 1.00 63.24 O ATOM 183 CB VAL A 10 4.962 8.793 -10.138 1.00 51.41 C ATOM 184 CG1 VAL A 10 4.966 10.308 -10.275 1.00 52.32 C ATOM 185 CG2 VAL A 10 4.339 8.371 -8.816 1.00 53.03 C ATOM 0 H VAL A 10 4.551 6.340 -10.307 1.00 11.34 H new ATOM 0 HA VAL A 10 3.196 8.532 -11.349 1.00 53.42 H new ATOM 0 HB VAL A 10 5.996 8.448 -10.153 1.00 51.41 H new ATOM 0 HG11 VAL A 10 5.499 10.748 -9.432 1.00 52.32 H new ATOM 0 HG12 VAL A 10 5.462 10.588 -11.204 1.00 52.32 H new ATOM 0 HG13 VAL A 10 3.940 10.675 -10.287 1.00 52.32 H new ATOM 0 HG21 VAL A 10 4.880 8.838 -7.993 1.00 53.03 H new ATOM 0 HG22 VAL A 10 3.296 8.685 -8.789 1.00 53.03 H new ATOM 0 HG23 VAL A 10 4.394 7.287 -8.717 1.00 53.03 H new ATOM 195 N ARG A 11 6.102 7.911 -12.821 1.00 32.32 N ATOM 196 CA ARG A 11 6.887 8.166 -14.022 1.00 41.13 C ATOM 197 C ARG A 11 6.052 7.930 -15.278 1.00 2.44 C ATOM 198 O ARG A 11 5.960 8.798 -16.145 1.00 52.04 O ATOM 199 CB ARG A 11 8.128 7.271 -14.046 1.00 54.24 C ATOM 200 CG ARG A 11 9.179 7.715 -15.050 1.00 62.12 C ATOM 201 CD ARG A 11 9.976 8.902 -14.534 1.00 4.34 C ATOM 202 NE ARG A 11 10.909 9.413 -15.535 1.00 20.31 N ATOM 203 CZ ARG A 11 10.546 10.192 -16.548 1.00 21.04 C ATOM 204 NH1 ARG A 11 9.277 10.549 -16.692 1.00 64.12 N ATOM 205 NH2 ARG A 11 11.453 10.616 -17.418 1.00 43.31 N ATOM 0 H ARG A 11 6.529 7.252 -12.170 1.00 32.32 H new ATOM 0 HA ARG A 11 7.201 9.210 -14.006 1.00 41.13 H new ATOM 0 HB2 ARG A 11 8.573 7.254 -13.051 1.00 54.24 H new ATOM 0 HB3 ARG A 11 7.825 6.250 -14.277 1.00 54.24 H new ATOM 0 HG2 ARG A 11 9.855 6.886 -15.262 1.00 62.12 H new ATOM 0 HG3 ARG A 11 8.696 7.981 -15.990 1.00 62.12 H new ATOM 0 HD2 ARG A 11 9.291 9.697 -14.238 1.00 4.34 H new ATOM 0 HD3 ARG A 11 10.528 8.607 -13.641 1.00 4.34 H new ATOM 0 HE ARG A 11 11.893 9.158 -15.452 1.00 20.31 H new ATOM 0 HH11 ARG A 11 8.577 10.225 -16.024 1.00 64.12 H new ATOM 0 HH12 ARG A 11 9.001 11.147 -17.471 1.00 64.12 H new ATOM 0 HH21 ARG A 11 12.430 10.344 -17.310 1.00 43.31 H new ATOM 0 HH22 ARG A 11 11.173 11.214 -18.196 1.00 43.31 H new ATOM 219 N ALA A 12 5.447 6.751 -15.367 1.00 32.34 N ATOM 220 CA ALA A 12 4.619 6.401 -16.514 1.00 51.52 C ATOM 221 C ALA A 12 3.459 7.379 -16.672 1.00 32.52 C ATOM 222 O ALA A 12 3.197 7.876 -17.768 1.00 20.01 O ATOM 223 CB ALA A 12 4.098 4.979 -16.376 1.00 21.41 C ATOM 0 H ALA A 12 5.515 6.021 -14.658 1.00 32.34 H new ATOM 0 HA ALA A 12 5.238 6.463 -17.409 1.00 51.52 H new ATOM 0 HB1 ALA A 12 3.481 4.732 -17.240 1.00 21.41 H new ATOM 0 HB2 ALA A 12 4.939 4.287 -16.321 1.00 21.41 H new ATOM 0 HB3 ALA A 12 3.500 4.897 -15.468 1.00 21.41 H new ATOM 229 N LEU A 13 2.766 7.649 -15.571 1.00 52.13 N ATOM 230 CA LEU A 13 1.632 8.567 -15.587 1.00 60.13 C ATOM 231 C LEU A 13 2.043 9.931 -16.132 1.00 64.01 C ATOM 232 O LEU A 13 1.271 10.593 -16.825 1.00 35.04 O ATOM 233 CB LEU A 13 1.055 8.720 -14.179 1.00 51.40 C ATOM 234 CG LEU A 13 -0.032 7.719 -13.786 1.00 41.12 C ATOM 235 CD1 LEU A 13 -0.259 7.741 -12.283 1.00 33.43 C ATOM 236 CD2 LEU A 13 -1.327 8.019 -14.528 1.00 25.23 C ATOM 0 H LEU A 13 2.969 7.246 -14.656 1.00 52.13 H new ATOM 0 HA LEU A 13 0.868 8.150 -16.243 1.00 60.13 H new ATOM 0 HB2 LEU A 13 1.872 8.638 -13.462 1.00 51.40 H new ATOM 0 HB3 LEU A 13 0.646 9.726 -14.083 1.00 51.40 H new ATOM 0 HG LEU A 13 0.301 6.720 -14.068 1.00 41.12 H new ATOM 0 HD11 LEU A 13 -1.036 7.022 -12.022 1.00 33.43 H new ATOM 0 HD12 LEU A 13 0.666 7.477 -11.771 1.00 33.43 H new ATOM 0 HD13 LEU A 13 -0.571 8.740 -11.977 1.00 33.43 H new ATOM 0 HD21 LEU A 13 -2.089 7.297 -14.236 1.00 25.23 H new ATOM 0 HD22 LEU A 13 -1.665 9.025 -14.278 1.00 25.23 H new ATOM 0 HD23 LEU A 13 -1.155 7.951 -15.602 1.00 25.23 H new TER 248 LEU A 13