USER MOD reduce.3.24.130724 H: found=0, std=0, add=128, rem=0, adj=5 USER MOD reduce.3.24.130724 removed 126 hydrogens (0 hets) USER MOD ----------------------------------------------------------------- USER MOD scores for adjustable sidechains, with "set" totals for H,N and Q USER MOD "o" means original, "f" means flipped, "180deg" is methyl default USER MOD "!" flags a clash with an overlap of 0.40A or greater USER MOD flip categories: "K"=keep, "C"=clashes, "X"=uncertain, "F"=flip USER MOD Single : A 1 VAL N :NH3+ -147:sc= -0.102 (180deg=-0.653) USER MOD Single : A 4 SER OG : rot 180:sc= 0 USER MOD Single : A 11 THR OG1 : rot 180:sc= 0 USER MOD Single : A 13 SER OG : rot 180:sc= 0 USER MOD Single : A 14 GLN : amide:sc= 0 X(o=0,f=0) USER MOD Single : A 15 LYS NZ :NH3+ 178:sc= 0 (180deg=-0.00348) USER MOD ----------------------------------------------------------------- ATOM 1 N VAL A 1 -0.103 -0.138 -0.404 1.00 74.21 N ATOM 2 CA VAL A 1 0.599 0.147 -1.650 1.00 31.04 C ATOM 3 C VAL A 1 0.757 -1.114 -2.492 1.00 51.54 C ATOM 4 O VAL A 1 0.503 -1.104 -3.697 1.00 41.32 O ATOM 5 CB VAL A 1 1.990 0.751 -1.384 1.00 4.34 C ATOM 6 CG1 VAL A 1 2.642 1.186 -2.687 1.00 51.44 C ATOM 7 CG2 VAL A 1 1.887 1.919 -0.414 1.00 23.21 C ATOM 0 H1 VAL A 1 -0.675 0.687 -0.131 1.00 74.21 H new ATOM 0 H2 VAL A 1 -0.724 -0.962 -0.536 1.00 74.21 H new ATOM 0 H3 VAL A 1 0.589 -0.342 0.345 1.00 74.21 H new ATOM 0 HA VAL A 1 -0.005 0.872 -2.196 1.00 31.04 H new ATOM 0 HB VAL A 1 2.618 -0.015 -0.930 1.00 4.34 H new ATOM 0 HG11 VAL A 1 3.624 1.610 -2.479 1.00 51.44 H new ATOM 0 HG12 VAL A 1 2.751 0.324 -3.345 1.00 51.44 H new ATOM 0 HG13 VAL A 1 2.018 1.936 -3.173 1.00 51.44 H new ATOM 0 HG21 VAL A 1 2.879 2.334 -0.237 1.00 23.21 H new ATOM 0 HG22 VAL A 1 1.242 2.689 -0.838 1.00 23.21 H new ATOM 0 HG23 VAL A 1 1.465 1.572 0.530 1.00 23.21 H new ATOM 17 N PHE A 2 1.178 -2.199 -1.851 1.00 14.30 N ATOM 18 CA PHE A 2 1.370 -3.468 -2.542 1.00 22.54 C ATOM 19 C PHE A 2 0.098 -3.889 -3.271 1.00 72.41 C ATOM 20 O PHE A 2 0.151 -4.400 -4.389 1.00 71.24 O ATOM 21 CB PHE A 2 1.785 -4.556 -1.548 1.00 44.33 C ATOM 22 CG PHE A 2 3.272 -4.751 -1.460 1.00 1.44 C ATOM 23 CD1 PHE A 2 4.113 -3.674 -1.227 1.00 34.03 C ATOM 24 CD2 PHE A 2 3.829 -6.010 -1.610 1.00 21.43 C ATOM 25 CE1 PHE A 2 5.481 -3.850 -1.144 1.00 41.11 C ATOM 26 CE2 PHE A 2 5.197 -6.192 -1.528 1.00 34.53 C ATOM 27 CZ PHE A 2 6.024 -5.111 -1.296 1.00 12.21 C ATOM 0 H PHE A 2 1.393 -2.225 -0.854 1.00 14.30 H new ATOM 0 HA PHE A 2 2.163 -3.336 -3.278 1.00 22.54 H new ATOM 0 HB2 PHE A 2 1.401 -4.301 -0.560 1.00 44.33 H new ATOM 0 HB3 PHE A 2 1.319 -5.498 -1.837 1.00 44.33 H new ATOM 0 HD1 PHE A 2 3.694 -2.686 -1.109 1.00 34.03 H new ATOM 0 HD2 PHE A 2 3.187 -6.859 -1.793 1.00 21.43 H new ATOM 0 HE1 PHE A 2 6.125 -3.003 -0.960 1.00 41.11 H new ATOM 0 HE2 PHE A 2 5.619 -7.179 -1.645 1.00 34.53 H new ATOM 0 HZ PHE A 2 7.093 -5.251 -1.234 1.00 12.21 H new ATOM 37 N ALA A 3 -1.045 -3.671 -2.629 1.00 20.12 N ATOM 38 CA ALA A 3 -2.331 -4.026 -3.216 1.00 11.10 C ATOM 39 C ALA A 3 -2.580 -3.248 -4.503 1.00 52.54 C ATOM 40 O ALA A 3 -3.095 -3.793 -5.480 1.00 1.52 O ATOM 41 CB ALA A 3 -3.453 -3.775 -2.220 1.00 33.04 C ATOM 0 H ALA A 3 -1.106 -3.250 -1.702 1.00 20.12 H new ATOM 0 HA ALA A 3 -2.309 -5.088 -3.462 1.00 11.10 H new ATOM 0 HB1 ALA A 3 -4.408 -4.044 -2.672 1.00 33.04 H new ATOM 0 HB2 ALA A 3 -3.290 -4.380 -1.328 1.00 33.04 H new ATOM 0 HB3 ALA A 3 -3.466 -2.720 -1.945 1.00 33.04 H new ATOM 47 N SER A 4 -2.213 -1.970 -4.498 1.00 24.22 N ATOM 48 CA SER A 4 -2.401 -1.116 -5.664 1.00 44.52 C ATOM 49 C SER A 4 -1.594 -1.632 -6.852 1.00 11.02 C ATOM 50 O SER A 4 -2.120 -1.786 -7.955 1.00 33.32 O ATOM 51 CB SER A 4 -1.990 0.322 -5.341 1.00 2.32 C ATOM 52 OG SER A 4 -1.829 1.085 -6.524 1.00 50.11 O ATOM 0 H SER A 4 -1.784 -1.504 -3.699 1.00 24.22 H new ATOM 0 HA SER A 4 -3.458 -1.134 -5.929 1.00 44.52 H new ATOM 0 HB2 SER A 4 -2.745 0.785 -4.706 1.00 2.32 H new ATOM 0 HB3 SER A 4 -1.057 0.319 -4.777 1.00 2.32 H new ATOM 0 HG SER A 4 -1.568 2.000 -6.290 1.00 50.11 H new ATOM 58 N LEU A 5 -0.314 -1.897 -6.618 1.00 64.54 N ATOM 59 CA LEU A 5 0.568 -2.397 -7.668 1.00 51.00 C ATOM 60 C LEU A 5 0.356 -3.891 -7.892 1.00 52.21 C ATOM 61 O LEU A 5 -0.098 -4.618 -7.008 1.00 41.21 O ATOM 62 CB LEU A 5 2.029 -2.126 -7.306 1.00 22.53 C ATOM 63 CG LEU A 5 2.455 -2.524 -5.892 1.00 31.40 C ATOM 64 CD1 LEU A 5 3.483 -3.644 -5.939 1.00 42.24 C ATOM 65 CD2 LEU A 5 3.008 -1.321 -5.143 1.00 2.20 C ATOM 0 H LEU A 5 0.137 -1.774 -5.711 1.00 64.54 H new ATOM 0 HA LEU A 5 0.326 -1.872 -8.592 1.00 51.00 H new ATOM 0 HB2 LEU A 5 2.663 -2.655 -8.017 1.00 22.53 H new ATOM 0 HB3 LEU A 5 2.223 -1.061 -7.436 1.00 22.53 H new ATOM 0 HG LEU A 5 1.577 -2.887 -5.358 1.00 31.40 H new ATOM 0 HD11 LEU A 5 3.774 -3.914 -4.924 1.00 42.24 H new ATOM 0 HD12 LEU A 5 3.052 -4.513 -6.436 1.00 42.24 H new ATOM 0 HD13 LEU A 5 4.361 -3.309 -6.491 1.00 42.24 H new ATOM 0 HD21 LEU A 5 3.306 -1.623 -4.139 1.00 2.20 H new ATOM 0 HD22 LEU A 5 3.874 -0.927 -5.675 1.00 2.20 H new ATOM 0 HD23 LEU A 5 2.241 -0.549 -5.077 1.00 2.20 H new ATOM 77 N PRO A 6 0.693 -4.362 -9.102 1.00 2.32 N ATOM 78 CA PRO A 6 0.551 -5.774 -9.469 1.00 41.45 C ATOM 79 C PRO A 6 1.546 -6.667 -8.736 1.00 40.41 C ATOM 80 O PRO A 6 2.654 -6.902 -9.216 1.00 21.44 O ATOM 81 CB PRO A 6 0.836 -5.779 -10.974 1.00 15.15 C ATOM 82 CG PRO A 6 1.693 -4.582 -11.201 1.00 63.30 C ATOM 83 CD PRO A 6 1.240 -3.552 -10.204 1.00 20.23 C ATOM 0 HA PRO A 6 -0.431 -6.167 -9.205 1.00 41.45 H new ATOM 0 HB2 PRO A 6 1.345 -6.694 -11.277 1.00 15.15 H new ATOM 0 HB3 PRO A 6 -0.086 -5.721 -11.552 1.00 15.15 H new ATOM 0 HG2 PRO A 6 2.746 -4.824 -11.060 1.00 63.30 H new ATOM 0 HG3 PRO A 6 1.584 -4.212 -12.221 1.00 63.30 H new ATOM 0 HD2 PRO A 6 2.067 -2.925 -9.870 1.00 20.23 H new ATOM 0 HD3 PRO A 6 0.487 -2.887 -10.627 1.00 20.23 H new ATOM 91 N GLY A 7 1.142 -7.162 -7.570 1.00 4.32 N ATOM 92 CA GLY A 7 2.011 -8.024 -6.789 1.00 53.34 C ATOM 93 C GLY A 7 1.235 -8.997 -5.923 1.00 44.53 C ATOM 94 O GLY A 7 0.198 -9.516 -6.337 1.00 42.41 O ATOM 0 H GLY A 7 0.229 -6.982 -7.152 1.00 4.32 H new ATOM 0 HA2 GLY A 7 2.664 -8.581 -7.461 1.00 53.34 H new ATOM 0 HA3 GLY A 7 2.653 -7.411 -6.156 1.00 53.34 H new ATOM 98 N ILE A 8 1.739 -9.246 -4.719 1.00 43.44 N ATOM 99 CA ILE A 8 1.087 -10.163 -3.794 1.00 20.24 C ATOM 100 C ILE A 8 0.371 -9.405 -2.681 1.00 24.12 C ATOM 101 O ILE A 8 0.371 -8.174 -2.655 1.00 44.04 O ATOM 102 CB ILE A 8 2.097 -11.142 -3.166 1.00 11.24 C ATOM 103 CG1 ILE A 8 3.110 -10.382 -2.307 1.00 42.11 C ATOM 104 CG2 ILE A 8 2.806 -11.940 -4.250 1.00 12.32 C ATOM 105 CD1 ILE A 8 4.135 -11.278 -1.647 1.00 41.51 C ATOM 0 H ILE A 8 2.597 -8.825 -4.362 1.00 43.44 H new ATOM 0 HA ILE A 8 0.357 -10.729 -4.373 1.00 20.24 H new ATOM 0 HB ILE A 8 1.556 -11.838 -2.526 1.00 11.24 H new ATOM 0 HG12 ILE A 8 3.626 -9.651 -2.929 1.00 42.11 H new ATOM 0 HG13 ILE A 8 2.576 -9.825 -1.537 1.00 42.11 H new ATOM 0 HG21 ILE A 8 3.516 -12.627 -3.790 1.00 12.32 H new ATOM 0 HG22 ILE A 8 2.072 -12.507 -4.823 1.00 12.32 H new ATOM 0 HG23 ILE A 8 3.338 -11.259 -4.914 1.00 12.32 H new ATOM 0 HD11 ILE A 8 4.820 -10.672 -1.054 1.00 41.51 H new ATOM 0 HD12 ILE A 8 3.629 -11.993 -0.998 1.00 41.51 H new ATOM 0 HD13 ILE A 8 4.695 -11.815 -2.412 1.00 41.51 H new ATOM 117 N ILE A 9 -0.236 -10.148 -1.762 1.00 21.21 N ATOM 118 CA ILE A 9 -0.953 -9.546 -0.645 1.00 3.03 C ATOM 119 C ILE A 9 -0.384 -10.016 0.690 1.00 14.42 C ATOM 120 O ILE A 9 -1.054 -9.945 1.721 1.00 24.13 O ATOM 121 CB ILE A 9 -2.456 -9.878 -0.695 1.00 63.13 C ATOM 122 CG1 ILE A 9 -2.669 -11.296 -1.230 1.00 12.22 C ATOM 123 CG2 ILE A 9 -3.193 -8.865 -1.557 1.00 53.54 C ATOM 124 CD1 ILE A 9 -1.991 -12.363 -0.400 1.00 21.13 C ATOM 0 H ILE A 9 -0.246 -11.168 -1.769 1.00 21.21 H new ATOM 0 HA ILE A 9 -0.825 -8.467 -0.733 1.00 3.03 H new ATOM 0 HB ILE A 9 -2.859 -9.827 0.317 1.00 63.13 H new ATOM 0 HG12 ILE A 9 -3.738 -11.504 -1.271 1.00 12.22 H new ATOM 0 HG13 ILE A 9 -2.295 -11.350 -2.252 1.00 12.22 H new ATOM 0 HG21 ILE A 9 -4.254 -9.113 -1.583 1.00 53.54 H new ATOM 0 HG22 ILE A 9 -3.063 -7.867 -1.137 1.00 53.54 H new ATOM 0 HG23 ILE A 9 -2.790 -8.888 -2.570 1.00 53.54 H new ATOM 0 HD11 ILE A 9 -2.185 -13.342 -0.838 1.00 21.13 H new ATOM 0 HD12 ILE A 9 -0.917 -12.180 -0.380 1.00 21.13 H new ATOM 0 HD13 ILE A 9 -2.382 -12.336 0.617 1.00 21.13 H new ATOM 136 N PHE A 10 0.855 -10.493 0.664 1.00 23.40 N ATOM 137 CA PHE A 10 1.515 -10.974 1.872 1.00 20.14 C ATOM 138 C PHE A 10 1.525 -9.896 2.952 1.00 3.11 C ATOM 139 O PHE A 10 1.147 -10.144 4.098 1.00 45.32 O ATOM 140 CB PHE A 10 2.947 -11.410 1.558 1.00 65.42 C ATOM 141 CG PHE A 10 3.516 -12.371 2.562 1.00 32.54 C ATOM 142 CD1 PHE A 10 3.173 -13.714 2.528 1.00 45.44 C ATOM 143 CD2 PHE A 10 4.392 -11.933 3.542 1.00 11.41 C ATOM 144 CE1 PHE A 10 3.695 -14.601 3.450 1.00 11.24 C ATOM 145 CE2 PHE A 10 4.917 -12.815 4.467 1.00 32.11 C ATOM 146 CZ PHE A 10 4.567 -14.151 4.422 1.00 10.51 C ATOM 0 H PHE A 10 1.423 -10.557 -0.181 1.00 23.40 H new ATOM 0 HA PHE A 10 0.955 -11.832 2.244 1.00 20.14 H new ATOM 0 HB2 PHE A 10 2.969 -11.873 0.571 1.00 65.42 H new ATOM 0 HB3 PHE A 10 3.585 -10.527 1.510 1.00 65.42 H new ATOM 0 HD1 PHE A 10 2.490 -14.071 1.772 1.00 45.44 H new ATOM 0 HD2 PHE A 10 4.668 -10.890 3.583 1.00 11.41 H new ATOM 0 HE1 PHE A 10 3.421 -15.645 3.411 1.00 11.24 H new ATOM 0 HE2 PHE A 10 5.600 -12.461 5.225 1.00 32.11 H new ATOM 0 HZ PHE A 10 4.974 -14.842 5.145 1.00 10.51 H new ATOM 156 N THR A 11 1.961 -8.697 2.579 1.00 44.25 N ATOM 157 CA THR A 11 2.023 -7.581 3.514 1.00 42.45 C ATOM 158 C THR A 11 0.652 -7.289 4.113 1.00 43.32 C ATOM 159 O THR A 11 0.534 -6.995 5.303 1.00 62.25 O ATOM 160 CB THR A 11 2.555 -6.306 2.832 1.00 0.30 C ATOM 161 OG1 THR A 11 2.516 -5.207 3.750 1.00 63.13 O ATOM 162 CG2 THR A 11 1.734 -5.970 1.596 1.00 11.02 C ATOM 0 H THR A 11 2.277 -8.474 1.635 1.00 44.25 H new ATOM 0 HA THR A 11 2.709 -7.872 4.309 1.00 42.45 H new ATOM 0 HB THR A 11 3.585 -6.487 2.526 1.00 0.30 H new ATOM 0 HG1 THR A 11 2.857 -4.401 3.310 1.00 63.13 H new ATOM 0 HG21 THR A 11 2.129 -5.066 1.132 1.00 11.02 H new ATOM 0 HG22 THR A 11 1.789 -6.796 0.887 1.00 11.02 H new ATOM 0 HG23 THR A 11 0.695 -5.807 1.883 1.00 11.02 H new ATOM 170 N ARG A 12 -0.381 -7.372 3.282 1.00 55.13 N ATOM 171 CA ARG A 12 -1.745 -7.116 3.730 1.00 2.03 C ATOM 172 C ARG A 12 -2.129 -8.058 4.868 1.00 42.13 C ATOM 173 O ARG A 12 -2.758 -7.646 5.842 1.00 23.25 O ATOM 174 CB ARG A 12 -2.726 -7.277 2.567 1.00 3.14 C ATOM 175 CG ARG A 12 -2.618 -6.177 1.523 1.00 33.45 C ATOM 176 CD ARG A 12 -3.989 -5.742 1.030 1.00 12.00 C ATOM 177 NE ARG A 12 -4.515 -4.617 1.798 1.00 53.41 N ATOM 178 CZ ARG A 12 -5.749 -4.144 1.658 1.00 62.01 C ATOM 179 NH1 ARG A 12 -6.579 -4.697 0.784 1.00 1.22 N ATOM 180 NH2 ARG A 12 -6.154 -3.117 2.393 1.00 15.42 N ATOM 0 H ARG A 12 -0.300 -7.615 2.295 1.00 55.13 H new ATOM 0 HA ARG A 12 -1.794 -6.091 4.097 1.00 2.03 H new ATOM 0 HB2 ARG A 12 -2.553 -8.240 2.087 1.00 3.14 H new ATOM 0 HB3 ARG A 12 -3.743 -7.295 2.960 1.00 3.14 H new ATOM 0 HG2 ARG A 12 -2.094 -5.321 1.948 1.00 33.45 H new ATOM 0 HG3 ARG A 12 -2.022 -6.530 0.681 1.00 33.45 H new ATOM 0 HD2 ARG A 12 -3.925 -5.464 -0.022 1.00 12.00 H new ATOM 0 HD3 ARG A 12 -4.681 -6.581 1.096 1.00 12.00 H new ATOM 0 HE ARG A 12 -3.902 -4.170 2.479 1.00 53.41 H new ATOM 0 HH11 ARG A 12 -6.271 -5.487 0.217 1.00 1.22 H new ATOM 0 HH12 ARG A 12 -7.526 -4.332 0.678 1.00 1.22 H new ATOM 0 HH21 ARG A 12 -5.518 -2.690 3.066 1.00 15.42 H new ATOM 0 HH22 ARG A 12 -7.101 -2.755 2.285 1.00 15.42 H new ATOM 194 N SER A 13 -1.746 -9.324 4.735 1.00 34.03 N ATOM 195 CA SER A 13 -2.054 -10.326 5.749 1.00 13.55 C ATOM 196 C SER A 13 -1.421 -9.956 7.087 1.00 31.25 C ATOM 197 O SER A 13 -2.085 -9.967 8.124 1.00 41.03 O ATOM 198 CB SER A 13 -1.562 -11.703 5.302 1.00 23.12 C ATOM 199 OG SER A 13 -1.851 -12.690 6.277 1.00 12.34 O ATOM 0 H SER A 13 -1.222 -9.680 3.935 1.00 34.03 H new ATOM 0 HA SER A 13 -3.136 -10.359 5.875 1.00 13.55 H new ATOM 0 HB2 SER A 13 -2.034 -11.972 4.357 1.00 23.12 H new ATOM 0 HB3 SER A 13 -0.487 -11.668 5.123 1.00 23.12 H new ATOM 0 HG SER A 13 -1.528 -13.561 5.966 1.00 12.34 H new ATOM 205 N GLN A 14 -0.133 -9.630 7.055 1.00 20.14 N ATOM 206 CA GLN A 14 0.591 -9.258 8.265 1.00 33.14 C ATOM 207 C GLN A 14 -0.032 -8.027 8.915 1.00 44.50 C ATOM 208 O GLN A 14 -0.196 -7.970 10.134 1.00 64.01 O ATOM 209 CB GLN A 14 2.062 -8.991 7.943 1.00 30.02 C ATOM 210 CG GLN A 14 2.932 -8.813 9.177 1.00 63.02 C ATOM 211 CD GLN A 14 3.308 -10.133 9.821 1.00 11.35 C ATOM 212 OE1 GLN A 14 4.146 -10.873 9.305 1.00 14.11 O ATOM 213 NE2 GLN A 14 2.689 -10.435 10.957 1.00 53.23 N ATOM 0 H GLN A 14 0.431 -9.616 6.205 1.00 20.14 H new ATOM 0 HA GLN A 14 0.526 -10.089 8.967 1.00 33.14 H new ATOM 0 HB2 GLN A 14 2.452 -9.819 7.350 1.00 30.02 H new ATOM 0 HB3 GLN A 14 2.134 -8.095 7.326 1.00 30.02 H new ATOM 0 HG2 GLN A 14 3.840 -8.275 8.903 1.00 63.02 H new ATOM 0 HG3 GLN A 14 2.403 -8.196 9.904 1.00 63.02 H new ATOM 0 HE21 GLN A 14 2.001 -9.792 11.349 1.00 53.23 H new ATOM 0 HE22 GLN A 14 2.902 -11.310 11.437 1.00 53.23 H new ATOM 222 N LYS A 15 -0.376 -7.041 8.093 1.00 13.13 N ATOM 223 CA LYS A 15 -0.982 -5.809 8.586 1.00 24.54 C ATOM 224 C LYS A 15 -2.301 -6.098 9.296 1.00 4.23 C ATOM 225 O LYS A 15 -2.550 -5.593 10.390 1.00 4.14 O ATOM 226 CB LYS A 15 -1.215 -4.833 7.431 1.00 21.31 C ATOM 227 CG LYS A 15 -1.832 -3.515 7.864 1.00 14.11 C ATOM 228 CD LYS A 15 -0.801 -2.598 8.500 1.00 20.33 C ATOM 229 CE LYS A 15 -1.149 -1.133 8.285 1.00 62.32 C ATOM 230 NZ LYS A 15 -2.402 -0.751 8.993 1.00 41.12 N ATOM 0 H LYS A 15 -0.245 -7.071 7.082 1.00 13.13 H new ATOM 0 HA LYS A 15 -0.296 -5.357 9.302 1.00 24.54 H new ATOM 0 HB2 LYS A 15 -0.264 -4.634 6.937 1.00 21.31 H new ATOM 0 HB3 LYS A 15 -1.865 -5.304 6.694 1.00 21.31 H new ATOM 0 HG2 LYS A 15 -2.277 -3.020 7.001 1.00 14.11 H new ATOM 0 HG3 LYS A 15 -2.638 -3.705 8.573 1.00 14.11 H new ATOM 0 HD2 LYS A 15 -0.738 -2.805 9.568 1.00 20.33 H new ATOM 0 HD3 LYS A 15 0.182 -2.805 8.077 1.00 20.33 H new ATOM 0 HE2 LYS A 15 -0.328 -0.509 8.638 1.00 62.32 H new ATOM 0 HE3 LYS A 15 -1.261 -0.939 7.218 1.00 62.32 H new ATOM 0 HZ1 LYS A 15 -2.587 0.262 8.850 1.00 41.12 H new ATOM 0 HZ2 LYS A 15 -3.197 -1.305 8.615 1.00 41.12 H new ATOM 0 HZ3 LYS A 15 -2.299 -0.944 10.010 1.00 41.12 H new