******** PROTEIN ********
Name: Thioredoxin h1
Organism: Arabidopsis thaliana
Gene: At3g51030.1
Expression system: Wheat germ cell-free system
PDB code: 1XFL
BMRB code: 6318
Sample conditions: 0.5 mM [U-15N; U-13C]At3g51030
50 mM KCl
10 mM phosphate buffer
pH 5.5, 298 K
******** NMR EXPERIMENTS ********
Spectrometer: Bruker DRX 600 MHz
HSQC: 15N HSQC, 13C aliphatic CT-HSQC, 13C aromatic CT- HSQC, 13C aromatic HSQC
Triple resonance: HNCA, HNCO, HNCOCA, HNCACO, CCONH, HCCH-TOCSY, HCCONH
NOESY: 3D 15N-NOESY, 3D 13C-NOESY aliphatic, 3D 13C-NOESY aromatic
--Each directory contains raw data, pulse sequences, acquisition parameters, and processing scripts
******** SOFTWARE ********
Collection: XWINNMR
Processing: NMRPipe
Analysis: XEASY
Peak picking: SPSCAN and XEASY
Backbone assignments: GARANT
Structure calculation: CYANA (CANDID used for automated NOE assignments)
Water refinement: XPLOR-NIH
******** CONTACT ********
Brian F. Volkman: bvolkman@biochem.mcw.edu
Directory Exp# Type
030804.3g51030 1 1D
030804.3g51030 2 15N HSQC
030804.3g51030 3 HNCO
030804.3g51030 4 15N HSQC
030804.3g51030 5 HNCA
030804.3g51030 6 15N HSQC
030804.3g51030 7 HNCOCA
030804.3g51030 8 15N HSQC
030804.3g51030 9 HNCACB
030804.3g51030 10 15N HSQC
030804.3g51030 11 HNCACO
030804.3g51030 12 15N HSQC
030804.3g51030 13 CCONH
030804.3g51030 14 15N HSQC
030804.3g51030 15 HCCH-Tocsy
030804.3g51030 16 15N HSQC
032504.3g51030 1 15N HSQC
032504.3g51030 3 13C CT-HSQC aromatic
032504.3g51030 6 13C CT-HSQC aliphatic
032504.3g51030 7 Aromatic HSQC
032504.3g51030 8 15N HSQC
041304.3g51030 1 1D
041304.3g51030 2 15N HSQC
041304.3g51030 3 HCCONH
041304.3g51030 4 13C NOESY aromatic
041504.3g51030 1 13C NOESY aliphatic
041704.3g51030 1 15N NOESY
******** PROTEIN ********
Name: Thioredoxin h1
Organism: Arabidopsis thaliana
Gene: At3g51030.1
Expression system: Wheat germ cell-free system
PDB code: 1XFL
BMRB code: 6318
Sample conditions: 0.5 mM [U-15N; U-13C]At3g51030
50 mM KCl
10 mM phosphate buffer
pH 5.5, 298 K
******** NMR EXPERIMENTS ********
Spectrometer: Bruker DRX 600 MHz
HSQC: 15N HSQC, 13C aliphatic CT-HSQC, 13C aromatic CT- HSQC, 13C aromatic HSQC
Triple resonance: HNCA, HNCO, HNCOCA, HNCACO, CCONH, HCCH-TOCSY, HCCONH
NOESY: 3D 15N-NOESY, 3D 13C-NOESY aliphatic, 3D 13C-NOESY aromatic
--Each directory contains raw data, pulse sequences, acquisition parameters, and processing scripts
******** SOFTWARE ********
Collection: XWINNMR
Processing: NMRPipe
Analysis: XEASY
Peak picking: SPSCAN and XEASY
Backbone assignments: GARANT
Structure calculation: CYANA (CANDID used for automated NOE assignments)
Water refinement: XPLOR-NIH
******** CONTACT ********
Brian F. Volkman: bvolkman@biochem.mcw.edu
Directory Exp# Type
030804.3g51030 1 1D
030804.3g51030 2 15N HSQC
030804.3g51030 3 HNCO
030804.3g51030 4 15N HSQC
030804.3g51030 5 HNCA
030804.3g51030 6 15N HSQC
030804.3g51030 7 HNCOCA
030804.3g51030 8 15N HSQC
030804.3g51030 9 HNCACB
030804.3g51030 10 15N HSQC
030804.3g51030 11 HNCACO
030804.3g51030 12 15N HSQC
030804.3g51030 13 CCONH
030804.3g51030 14 15N HSQC
030804.3g51030 15 HCCH-Tocsy
030804.3g51030 16 15N HSQC
032504.3g51030 1 15N HSQC
032504.3g51030 3 13C CT-HSQC aromatic
032504.3g51030 6 13C CT-HSQC aliphatic
032504.3g51030 7 Aromatic HSQC
032504.3g51030 8 15N HSQC
041304.3g51030 1 1D
041304.3g51030 2 15N HSQC
041304.3g51030 3 HCCONH
041304.3g51030 4 13C NOESY aromatic
041504.3g51030 1 13C NOESY aliphatic
041704.3g51030 1 15N NOESY