********  PROTEIN ********

Name:  			N-terminal ubiquitin-like domain of tubulin-folding 
			cofactor B
Organism: 		Caenorhabditis elegans
Gene:			Ce5O73 or F53F4.3
Expression system:      E. coli/pQE30T
PDB code:		1T0Y
BMRB code:		6176
Sample conditions:	1.0 mM [U-15N; U-13C]ubiquitin-like domain
			50 mM NaCl
			20 mM sodium phosphate buffer
			10% D20/90% H20
			pH 6.5, 298 K

******** NMR EXPERIMENTS ********

Spectrometer:		Bruker DRX 600 MHz

HSQC:			15N HSQC, 13C aliphatic CT-HSQC, 13C aromatic CT-HSQC,
			13C aromatic HSQC
Triple resonance:	HNCA, HNCO, HNCOCA, HNCACO, CCONH, HCCH-TOCSY, 
			3D 15N-TOCSY
NOESY:			3D 15N-NOESY, 3D 13C-NOESY, 3D 13C-NOESY aromatic
Other:			Diffusion, hetNOE

--Each directory contains raw data, pulse sequences, acquisition parameters, 
  and processing scripts (if used)

******** SOFTWARE ********

Collection:		XWINNMR
Processing:		NMRPipe
Analysis:		XEASY
Peak picking:		SPSCAN and XEASY
Backbone assignments:	GARANT
Structure calculation:	CYANA (CANDID used for automated NOE assignments)
Water refinement:	XPLOR-NIH

******** CONTACT ********

Brian F. Volkman:	bvolkman@biochem.mcw.edu







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			Sample
Directory	Exp #	Jar ID	Experiment

082203.celeg	1	3766	1D - No decoupling
082203.celeg	2	3766	1D - decoupled
082203.celeg	3	3766	15N HSQC
082203.celeg	4	3766	15N Noesy
082203.celeg	5	3766	15N HSQC
082203.celeg	6	3766	15N Tocsy
082203.celeg	7	3766	15N HSQC
			
091103.celeg	1	3766	1D - No decoupling
091103.celeg	2	3766	1D - decoupled
091103.celeg	3	3766	15N HSQC
091103.celeg	4	3766	15N HSQC
091103.celeg	5	3766	15N Tocsy
091103.celeg	6	3766	15N HSQC
			
091203.celeg	1	3771	1D - decoupled
091203.celeg	2	3771	15N HSQC
091203.celeg	3	3771	HNCO
091203.celeg	4	3771	HNCA
091203.celeg	5	3771	15N HSQC
091203.celeg	6	3771	HNCOCA
091203.celeg	7	3771	HNCACB
091203.celeg	8	3771	15N HSQC
091203.celeg	9	3771	HNCACO
091203.celeg	10	3771	15N HSQC
			
091503.celeg	1	3771	CCONH
091503.celeg	2	3771	15N HSQC
091503.celeg	3	3771	HCCH-Tocsy
			
091703.celeg	1	3771	15N HSQC
091703.celeg	2	3771	13C CT-HSQC alaphatic
091703.celeg	3	3771	13C CT-HSQC aromatic
091703.celeg	4	3771	13C aromatic HSQC
091703.celeg	5	3771	13C aromatic Noesy
091703.celeg	6	3771	15N HSQC
			
091803.celeg	1	3771	15N HSQC
091803.celeg	2	3771	13C Noesy
091803.celeg	3	3771	15N HSQC
			
092103.celeg	1	3766	15N HSQC
092103.celeg	2	3766	15N hetNOE
092103.celeg	3	3766	15N HSQC
092103.celeg	4	3766	15N Noesy
092103.celeg	5	3766	15N HSQC
092103.celeg	10	3766	hetNOE data
092103.celeg	11	3766	hetNOE data
			
092503.celeg	1	3766	1D
092503.celeg	2	3766	Diffusion
092503.celeg	3	3766	Diffusion