******** PROTEIN ********
Name: N-terminal ubiquitin-like domain of tubulin-folding
cofactor B
Organism: Caenorhabditis elegans
Gene: Ce5O73 or F53F4.3
Expression system: E. coli/pQE30T
PDB code: 1T0Y
BMRB code: 6176
Sample conditions: 1.0 mM [U-15N; U-13C]ubiquitin-like domain
50 mM NaCl
20 mM sodium phosphate buffer
10% D20/90% H20
pH 6.5, 298 K
******** NMR EXPERIMENTS ********
Spectrometer: Bruker DRX 600 MHz
HSQC: 15N HSQC, 13C aliphatic CT-HSQC, 13C aromatic CT-HSQC,
13C aromatic HSQC
Triple resonance: HNCA, HNCO, HNCOCA, HNCACO, CCONH, HCCH-TOCSY,
3D 15N-TOCSY
NOESY: 3D 15N-NOESY, 3D 13C-NOESY, 3D 13C-NOESY aromatic
Other: Diffusion, hetNOE
--Each directory contains raw data, pulse sequences, acquisition parameters,
and processing scripts (if used)
******** SOFTWARE ********
Collection: XWINNMR
Processing: NMRPipe
Analysis: XEASY
Peak picking: SPSCAN and XEASY
Backbone assignments: GARANT
Structure calculation: CYANA (CANDID used for automated NOE assignments)
Water refinement: XPLOR-NIH
******** CONTACT ********
Brian F. Volkman: bvolkman@biochem.mcw.edu
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#
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Sample
Directory Exp # Jar ID Experiment
082203.celeg 1 3766 1D - No decoupling
082203.celeg 2 3766 1D - decoupled
082203.celeg 3 3766 15N HSQC
082203.celeg 4 3766 15N Noesy
082203.celeg 5 3766 15N HSQC
082203.celeg 6 3766 15N Tocsy
082203.celeg 7 3766 15N HSQC
091103.celeg 1 3766 1D - No decoupling
091103.celeg 2 3766 1D - decoupled
091103.celeg 3 3766 15N HSQC
091103.celeg 4 3766 15N HSQC
091103.celeg 5 3766 15N Tocsy
091103.celeg 6 3766 15N HSQC
091203.celeg 1 3771 1D - decoupled
091203.celeg 2 3771 15N HSQC
091203.celeg 3 3771 HNCO
091203.celeg 4 3771 HNCA
091203.celeg 5 3771 15N HSQC
091203.celeg 6 3771 HNCOCA
091203.celeg 7 3771 HNCACB
091203.celeg 8 3771 15N HSQC
091203.celeg 9 3771 HNCACO
091203.celeg 10 3771 15N HSQC
091503.celeg 1 3771 CCONH
091503.celeg 2 3771 15N HSQC
091503.celeg 3 3771 HCCH-Tocsy
091703.celeg 1 3771 15N HSQC
091703.celeg 2 3771 13C CT-HSQC alaphatic
091703.celeg 3 3771 13C CT-HSQC aromatic
091703.celeg 4 3771 13C aromatic HSQC
091703.celeg 5 3771 13C aromatic Noesy
091703.celeg 6 3771 15N HSQC
091803.celeg 1 3771 15N HSQC
091803.celeg 2 3771 13C Noesy
091803.celeg 3 3771 15N HSQC
092103.celeg 1 3766 15N HSQC
092103.celeg 2 3766 15N hetNOE
092103.celeg 3 3766 15N HSQC
092103.celeg 4 3766 15N Noesy
092103.celeg 5 3766 15N HSQC
092103.celeg 10 3766 hetNOE data
092103.celeg 11 3766 hetNOE data
092503.celeg 1 3766 1D
092503.celeg 2 3766 Diffusion
092503.celeg 3 3766 Diffusion
******** PROTEIN ********
Name: N-terminal ubiquitin-like domain of tubulin-folding
cofactor B
Organism: Caenorhabditis elegans
Gene: Ce5O73 or F53F4.3
Expression system: E. coli/pQE30T
PDB code: 1T0Y
BMRB code: 6176
Sample conditions: 1.0 mM [U-15N; U-13C]ubiquitin-like domain
50 mM NaCl
20 mM sodium phosphate buffer
10% D20/90% H20
pH 6.5, 298 K
******** NMR EXPERIMENTS ********
Spectrometer: Bruker DRX 600 MHz
HSQC: 15N HSQC, 13C aliphatic CT-HSQC, 13C aromatic CT-HSQC,
13C aromatic HSQC
Triple resonance: HNCA, HNCO, HNCOCA, HNCACO, CCONH, HCCH-TOCSY,
3D 15N-TOCSY
NOESY: 3D 15N-NOESY, 3D 13C-NOESY, 3D 13C-NOESY aromatic
Other: Diffusion, hetNOE
--Each directory contains raw data, pulse sequences, acquisition parameters,
and processing scripts (if used)
******** SOFTWARE ********
Collection: XWINNMR
Processing: NMRPipe
Analysis: XEASY
Peak picking: SPSCAN and XEASY
Backbone assignments: GARANT
Structure calculation: CYANA (CANDID used for automated NOE assignments)
Water refinement: XPLOR-NIH
******** CONTACT ********
Brian F. Volkman: bvolkman@biochem.mcw.edu
#
#
#
Sample
Directory Exp # Jar ID Experiment
082203.celeg 1 3766 1D - No decoupling
082203.celeg 2 3766 1D - decoupled
082203.celeg 3 3766 15N HSQC
082203.celeg 4 3766 15N Noesy
082203.celeg 5 3766 15N HSQC
082203.celeg 6 3766 15N Tocsy
082203.celeg 7 3766 15N HSQC
091103.celeg 1 3766 1D - No decoupling
091103.celeg 2 3766 1D - decoupled
091103.celeg 3 3766 15N HSQC
091103.celeg 4 3766 15N HSQC
091103.celeg 5 3766 15N Tocsy
091103.celeg 6 3766 15N HSQC
091203.celeg 1 3771 1D - decoupled
091203.celeg 2 3771 15N HSQC
091203.celeg 3 3771 HNCO
091203.celeg 4 3771 HNCA
091203.celeg 5 3771 15N HSQC
091203.celeg 6 3771 HNCOCA
091203.celeg 7 3771 HNCACB
091203.celeg 8 3771 15N HSQC
091203.celeg 9 3771 HNCACO
091203.celeg 10 3771 15N HSQC
091503.celeg 1 3771 CCONH
091503.celeg 2 3771 15N HSQC
091503.celeg 3 3771 HCCH-Tocsy
091703.celeg 1 3771 15N HSQC
091703.celeg 2 3771 13C CT-HSQC alaphatic
091703.celeg 3 3771 13C CT-HSQC aromatic
091703.celeg 4 3771 13C aromatic HSQC
091703.celeg 5 3771 13C aromatic Noesy
091703.celeg 6 3771 15N HSQC
091803.celeg 1 3771 15N HSQC
091803.celeg 2 3771 13C Noesy
091803.celeg 3 3771 15N HSQC
092103.celeg 1 3766 15N HSQC
092103.celeg 2 3766 15N hetNOE
092103.celeg 3 3766 15N HSQC
092103.celeg 4 3766 15N Noesy
092103.celeg 5 3766 15N HSQC
092103.celeg 10 3766 hetNOE data
092103.celeg 11 3766 hetNOE data
092503.celeg 1 3766 1D
092503.celeg 2 3766 Diffusion
092503.celeg 3 3766 Diffusion