********  PROTEIN ********

Name:  			At3g01050.1
Organism: 		Arabidopsis thaliana
Expression system:      Wheat germ cell-free system
PDB code:		1se9
BMRB code:		6128
Sample conditions:	0.5 mM [U-15N; U-13C]At3g01050
			50 mM KCl
			10 mM phosphate buffer
			1 mM DTT
			pH 6.5, 298 K

******** NMR EXPERIMENTS ********

Spectrometer:		Bruker DRX 600 MHz

HSQC:			15N HSQC, 13C aliphatic CT-HSQC, 13C aromatic CT-HSQC,
			13C aromatic HSQC
Triple resonance:	HNCA, HNCO, HNCOCA, HNCACO, CCONH, HCCH-TOCSY, 
			3D 15N-TOCSY
NOESY:			3D 15N-NOESY, 3D 13C-NOESY, 3D 13C-NOESY aromatic
Other:			Diffusion, hetNOE

--Each directory contains raw data, pulse sequences, acquisition parameters, 
  and processing scripts (if used)

******** SOFTWARE ********

Collection:		XWINNMR
Processing:		NMRPipe
Analysis:		XEASY
Peak picking:		SPSCAN and XEASY
Backbone assignments:	GARANT
Structure calculation:	CYANA (CANDID used for automated NOE assignments)
Water refinement:	XPLOR-NIH

******** CONTACT ********

Brian F. Volkman:	bvolkman@biochem.mcw.edu

			Sample		
Directory	Exp #	Jar ID	Experiment		Comments

071103.3g01050	1		1D	
071103.3g01050	2		15N HSQC		weak sample
071103.3g01050	3		15N Noesy		no cross-peaks
071103.3g01050	4		15N HSQC	
071103.3g01050	11		1D			run 07-17-03
071103.3g01050	12		15N HSQC		run 07-17-03
				
071103.3g01050	1	3391	1D	
071103.3g01050	2	3391	15N HSQC		weaker than 15N sample
071103.3g01050	3	3391	HNCA			crashed
071103.3g01050	4	3391	HNCA	
071103.3g01050	5	3391	15N HSQC	
071103.3g01050	6	3391	HNCO	
				
071703.3g01050	1	3392	1D	
071703.3g01050	2	3392	15N HSQC	
071703.3g01050	3	3392	HNCA			used for structure determination
071703.3g01050	4	3392	HNCO			used for structure determination
071703.3g01050	5	3392	15N HSQC		used for structure determination
071703.3g01050	6	3392	HNCOCA			used for structure determination
071703.3g01050	7	3392	15N HSQC	
071703.3g01050	8	3392	HNCACB			used for structure determination
071703.3g01050	9	3392	15N HSQC	
071703.3g01050	10	3392	HNCACO			used for structure determination
071703.3g01050	11	3392	15N HSQC	
071703.3g01050	12	3392	15N-TOCSY		used for structure determination
071703.3g01050	13	3392	15N HSQC	
				
072103.3g01050	2	3392	CCONH			used for structure determination
072103.3g01050	3	3392	HCCH-Tocsy		used for structure determination
072103.3g01050	4	3392	15N HSQC	
072103.3g01050	10	3392	1D	
				
072303.3g01050	1	3458	1D	
072303.3g01050	2	3458	15N HSQC	
072303.3g01050	3	3458	15N HSQC	
				
072403.3g01050	1	3458	1D	
072403.3g01050	2	3458	15N HSQC	
072403.3g01050	3	3458	15N HSQC	
072403.3g01050	4	3458	HCCH-Tocsy		used for structure determination
				
072503.3g01050	1	3458	13C alaphatic CT-HSQC	not used - wrong bf2
072503.3g01050	2	3458	13C Noesy		used for structure determination
072503.3g01050	3	3458	13C aromatic CT-HSQC	used for structure determination
072503.3g01050	4	3458	15N HSQC	
072503.3g01050	9	3458	15N HSQC	
072503.3g01050	10	3458	15N Noesy 		not used - no 13C decoupling
072503.3g01050	11	3458	15N HSQC	
072503.3g01050	12	3458	13C aromatic HSQC	used for structure determination
				
073103.3g01050	1	3458	15N HSQC	
073103.3g01050	2	3458	CCONH			used for structure determination
073103.3g01050	3	3458	13C alaphatic CT-HSQC	used for structure determination
073103.3g01050	11	3458	1D	
073103.3g01050	12	3458	Diffusion	
073103.3g01050	13	3458	1D	
073103.3g01050	14	3458	Diffusion	
				
080103.3g01050	1	3458	15N HSQC	
080103.3g01050	2	3458	15N Noesy	u	sed for structure determination
080103.3g01050	3	3458	13C aromatic Noesy	used for structure determination
080103.3g01050	4	3458	15N HSQC	
080103.3g01050	5	3458	HNCA			used for structure determination
080103.3g01050	6	3458	HNCO			used for structure determination
080103.3g01050	7	3458	HNCOCA			used for structure determination
080103.3g01050	8	3458	15N HSQC	
				
080606.3g01050	1	3458	1D	
080606.3g01050	2	3458	15N HSQC	
080606.3g01050	3	3458	13C aromatic Noesy	
080606.3g01050	4	3458	15N HSQC	
				
082903.3g01050	1	3458	15N HSQC	
082903.3g01050	2	3458	15N Noesy	
082903.3g01050	3	3458	15N HSQC	
				
110603.3g01050	1	4126	1D	
110603.3g01050	2	4126	15N HSQC	
110603.3g01050	3	4126	15N hetNOE	
110603.3g01050	4	4126	15N HSQC	
110603.3g01050	5	4126	15N Noesy	
110603.3g01050	6	4126	15N HSQC	
110603.3g01050	10	4126	hetNOE data	
110603.3g01050	11	4126	hetNOE data
[ICO]NameLast modifiedSizeDescription
[PARENTDIR]Parent Directory  -  
[DIR]071003.3g01050/2021-02-13 18:34 -  
[DIR]071103.3g01050/2021-02-13 18:33 -  
[DIR]071703.3g01050/2021-02-13 18:34 -  
[DIR]072103.3g01050/2021-02-13 18:34 -  
[DIR]072303.3g01050/2021-02-13 18:33 -  
[DIR]072403.3g01050/2021-02-13 18:34 -  
[DIR]073103.3g01050/2021-02-13 18:34 -  
[DIR]080103.3g01050/2021-02-13 18:34 -  
[DIR]082903.3g01050/2021-02-13 18:34 -  
[DIR]110603.3g01050/2021-02-13 18:34 -  
[ ]At3g01050_timedomain.tar.gz2021-02-13 18:34 460M 
[TXT]README.txt2021-02-13 18:34 4.2K 
[DIR]analysis/2021-02-13 18:36 -  
********  PROTEIN ********

Name:  			At3g01050.1
Organism: 		Arabidopsis thaliana
Expression system:      Wheat germ cell-free system
PDB code:		1se9
BMRB code:		6128
Sample conditions:	0.5 mM [U-15N; U-13C]At3g01050
			50 mM KCl
			10 mM phosphate buffer
			1 mM DTT
			pH 6.5, 298 K

******** NMR EXPERIMENTS ********

Spectrometer:		Bruker DRX 600 MHz

HSQC:			15N HSQC, 13C aliphatic CT-HSQC, 13C aromatic CT-HSQC,
			13C aromatic HSQC
Triple resonance:	HNCA, HNCO, HNCOCA, HNCACO, CCONH, HCCH-TOCSY, 
			3D 15N-TOCSY
NOESY:			3D 15N-NOESY, 3D 13C-NOESY, 3D 13C-NOESY aromatic
Other:			Diffusion, hetNOE

--Each directory contains raw data, pulse sequences, acquisition parameters, 
  and processing scripts (if used)

******** SOFTWARE ********

Collection:		XWINNMR
Processing:		NMRPipe
Analysis:		XEASY
Peak picking:		SPSCAN and XEASY
Backbone assignments:	GARANT
Structure calculation:	CYANA (CANDID used for automated NOE assignments)
Water refinement:	XPLOR-NIH

******** CONTACT ********

Brian F. Volkman:	bvolkman@biochem.mcw.edu

			Sample		
Directory	Exp #	Jar ID	Experiment		Comments

071103.3g01050	1		1D	
071103.3g01050	2		15N HSQC		weak sample
071103.3g01050	3		15N Noesy		no cross-peaks
071103.3g01050	4		15N HSQC	
071103.3g01050	11		1D			run 07-17-03
071103.3g01050	12		15N HSQC		run 07-17-03
				
071103.3g01050	1	3391	1D	
071103.3g01050	2	3391	15N HSQC		weaker than 15N sample
071103.3g01050	3	3391	HNCA			crashed
071103.3g01050	4	3391	HNCA	
071103.3g01050	5	3391	15N HSQC	
071103.3g01050	6	3391	HNCO	
				
071703.3g01050	1	3392	1D	
071703.3g01050	2	3392	15N HSQC	
071703.3g01050	3	3392	HNCA			used for structure determination
071703.3g01050	4	3392	HNCO			used for structure determination
071703.3g01050	5	3392	15N HSQC		used for structure determination
071703.3g01050	6	3392	HNCOCA			used for structure determination
071703.3g01050	7	3392	15N HSQC	
071703.3g01050	8	3392	HNCACB			used for structure determination
071703.3g01050	9	3392	15N HSQC	
071703.3g01050	10	3392	HNCACO			used for structure determination
071703.3g01050	11	3392	15N HSQC	
071703.3g01050	12	3392	15N-TOCSY		used for structure determination
071703.3g01050	13	3392	15N HSQC	
				
072103.3g01050	2	3392	CCONH			used for structure determination
072103.3g01050	3	3392	HCCH-Tocsy		used for structure determination
072103.3g01050	4	3392	15N HSQC	
072103.3g01050	10	3392	1D	
				
072303.3g01050	1	3458	1D	
072303.3g01050	2	3458	15N HSQC	
072303.3g01050	3	3458	15N HSQC	
				
072403.3g01050	1	3458	1D	
072403.3g01050	2	3458	15N HSQC	
072403.3g01050	3	3458	15N HSQC	
072403.3g01050	4	3458	HCCH-Tocsy		used for structure determination
				
072503.3g01050	1	3458	13C alaphatic CT-HSQC	not used - wrong bf2
072503.3g01050	2	3458	13C Noesy		used for structure determination
072503.3g01050	3	3458	13C aromatic CT-HSQC	used for structure determination
072503.3g01050	4	3458	15N HSQC	
072503.3g01050	9	3458	15N HSQC	
072503.3g01050	10	3458	15N Noesy 		not used - no 13C decoupling
072503.3g01050	11	3458	15N HSQC	
072503.3g01050	12	3458	13C aromatic HSQC	used for structure determination
				
073103.3g01050	1	3458	15N HSQC	
073103.3g01050	2	3458	CCONH			used for structure determination
073103.3g01050	3	3458	13C alaphatic CT-HSQC	used for structure determination
073103.3g01050	11	3458	1D	
073103.3g01050	12	3458	Diffusion	
073103.3g01050	13	3458	1D	
073103.3g01050	14	3458	Diffusion	
				
080103.3g01050	1	3458	15N HSQC	
080103.3g01050	2	3458	15N Noesy	u	sed for structure determination
080103.3g01050	3	3458	13C aromatic Noesy	used for structure determination
080103.3g01050	4	3458	15N HSQC	
080103.3g01050	5	3458	HNCA			used for structure determination
080103.3g01050	6	3458	HNCO			used for structure determination
080103.3g01050	7	3458	HNCOCA			used for structure determination
080103.3g01050	8	3458	15N HSQC	
				
080606.3g01050	1	3458	1D	
080606.3g01050	2	3458	15N HSQC	
080606.3g01050	3	3458	13C aromatic Noesy	
080606.3g01050	4	3458	15N HSQC	
				
082903.3g01050	1	3458	15N HSQC	
082903.3g01050	2	3458	15N Noesy	
082903.3g01050	3	3458	15N HSQC	
				
110603.3g01050	1	4126	1D	
110603.3g01050	2	4126	15N HSQC	
110603.3g01050	3	4126	15N hetNOE	
110603.3g01050	4	4126	15N HSQC	
110603.3g01050	5	4126	15N Noesy	
110603.3g01050	6	4126	15N HSQC	
110603.3g01050	10	4126	hetNOE data	
110603.3g01050	11	4126	hetNOE data