Gallium protoporphyrin IX-HasAsm complex

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1H, 15N and 13C resonance assignments for the gallium protoporphyrin IX-HasA(sm) hemophore complex.

Simple procedures are described for recording complementary in-phase and antiphase J-coupled NMR spectra. The sum and difference of these spectra contain only the upfield and the downfield components of a doublet, making it possible to measure the J splitting directly from these combinations without an increase in resonance overlap relative to the decoupled spectrum. The approach is demonstrated for measurement of 1JNH splittings and 2JHNC splittings in oriented and isotropic ubiquitin. Dipolar couplings obtained from differences in the splittings measured in the oriented and isotropic phases are in excellent agreement with dipolar couplings obtained from direct measurement of the splitting or from a conventional E. COSY-type measurement.

Ottiger M, Delaglio F, Bax A.
Measurement of J and dipolar couplings from simplified
two-dimensional NMR spectra.
J. Magn. Reson. 1998 Apr;131(2):373-8.

Journal of magnetic resonance (San Diego, Calif. : 1997)
Measurement of J and dipolar couplings from simplified two-dimensional NMR spectra.

Bartels, C., Xia, T., Billeter, M., Guntert, P. and Wuthrich, K.
J. Biomol. NMR, 5, 1-10 (1995).

Hemophore HasA (Heme acquisition system protein A)

Crystal Structure Of The Smhasa Mutant H83a

The Crystal Structure Of The Secreted Dimeric Form Of The Hemophore Hasa Reveals A Domain Swapping With An Exchanged Heme Ligand

Structural And Dynamics Studies Of Both Apo And Holo Forms Of The Hemophore Hasa

Crystal Structure Of The Hemophore Hasa, Ph 6.5

Crystal Structure Of The Hemophore Hasa From Serratia Marcescens Crystal Form P2(1), Ph8

1H, 15N and 13C resonance assignments for the Gallium protoporphyrin IX-HasAsm hemophore complex

Trosy options were used for 1H-15N HSQC, HNCO, HNCACB and
CBCA(CO)NH experiments.
A triple resonance z-gradient probe was employed.