Structure of the BamA C-terminus extend barrel

Journal of the American Chemical Society
Sequence-specific solution NMR assignments of the β-barrel insertase BamA to monitor its conformational ensemble at the atomic level.

Backbone 1H, 13C, and 15N Chemical Shift Assignments of the BamA barrel from E.coli with a C-terminal extension.
Backbone 1H, 13C, and 15N Chemical Shift Assignments for BamA barrel of E.coli

Crystal structure of E.coli C-ter extended BamA barrel

Culture supplemented with 14N Serine and Glycine to prevent scrambling

Threonine was labeled, and allowed to scramble to serines and glycines. Threonines and glycines were identified from their respective spectra. Remaining peaks were identified as serines.