An exon splice enhancer primes IGF2:IGF2R binding site structure and function evolution.

Residue 1489 = M rather than N (first residue).

Nmr Solution Structure Ensemble Of Domain 11 Of The Echidna M6pIGF2R Receptor

Obtained from liver tissue of the echidna.

Echidna (Tachyglossus aculeatus) domain 11 of the mannose-6-phosphate/insulin-like growth factor 2 receptor (M6P/IGF2R).
NMR solution structure ensemble of domain 11 of the echidna M6P/IGF2R receptor

The structure was determined using a combination of NOE restraints, dihedral restraints predicted from TALOS and hydrogen bond restraints initially predicted from the homologous human domain 11 IGF2R structure (2CNJ).

DGSA-distance geometry simulated annealing
NMR STUDIES ON THE INTERACTION OF INSULIN-GROWTH FACTOR II (IGF-II) WITH IGF2R DOMAIN 11
Software for structural calculations from dihedral, hydrogen bond and distance restraints. RECOORD water refinement also used within the program.
Torsion Angle Likelihood Obtained from Shift and sequence similarity - dihedral angles predicted.
Used partially for some chemical shift assignment. Assignment checked manually afterwards.
Validation after each iteration of ARIA.
Used to peak pick and assign all assigned atoms. Also used as a format converter.
Online portal used to calculate 3D structure from chemical shift data. Model shown to be homologous to human domain 11 IGF2R
Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax
Wishart, Arndt, Berjanskii, Tang, Zhou and Lin
Department of Computing Science, Department of Biological Sciences, University of Alberta and National Research Council, National Institute for Nanotechnology (NINT), Edmonton, AB, Canada T6G 2E8.