3-{[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl]sulfanyl}-3-oxopropanoic acid
THIOMALONIC ACID S-{2-[3-(2-HYDROXY-3,3-DIMETHYL-4-PHOSPHONOOXY-BUTYRYLAMINO)-PROPIONYLAMINO]-ETHYL} ESTER

3-[2-[3-[[(2S)-2-hydroxy-3,3-dimethyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethylsulfanyl]-3-oxo-propanoic acid

Probing the Interactions of early polyketide intermediates with the Actinorhodin ACP from S. coelicolor A3(2).

The 42nd residue, SXM is 4'-phosphopantetheine_acylated_with_a_malonyl_group

Actinorhodin Polyketide Synthase Acyl Carrier Protein From Streptomyces Coelicolor A3(2), Nmr, 24 Structures

Actinorhodin Polyketide Synthase Acyl Carrier Protein From Streptomyces Coelicolor A3(2), Nmr, Minimized Average Structure

The Actinorhodin Holo Acyl Carrier Protein From S. Coelicolor

The Actinorhodin Apo Acyl Carrier Protein From S. Coelicolor

Solution Structure Of The Acetyl Actinorhodin Acyl Carrier Protein From Streptomyces Coelicolor

Nmr Solution Structures Of Malonyl Acp From The Actinorhodin Polyketide Synthase In Streptomyces Coelicolor

Nmr Solution Structures Of Butyryl-Acp (A Non-Polar, Non Pathway Intermediate) From The Actinorhodin Polyketide Synthase In Str

Nmr Solution Structures Of Hexanoyl Acp (A Non Natural Intermediate) From The Actinorhodin Polyketide Synthase In Streptomyces

Nmr Solution Structures Of Octanoyl Acp (A Non-Natural Intermediate) From The Actinorhodin Polyketide Synthase In Streptomyces

Nmr Solution Structures Of 3-Oxo-Butyl-Acp, An Intermediate Mimic From The Actinorhodin Polyketide Synthase In Streptomyces Coe

Nmr Solution Structures Of 3,5-Dioxohexyl Acp (A Triketide Mimic) From The Actinorhodin Polyketide Synthase In Streptomyces Coe

Solution Structure Of The 3,7-dioxo-octyl Actinorhodin Acyl Carrier Protein From Streptomyces Coelicolor

Solution Structure Of The 5-phenyl-3-oxo-pentyl Actinorhodin Acyl Carrier Protein From Streptomyces Coelicolor

Acyl carrier protein [Streptomyces coelicolor A3(2)]

actinorhodin polyketide synthase acyl carrier protein [Streptomyces coelicolor A3(2)]

actinorhodin polyketide synthase ACP [Streptomyces lividans TK24]

acyl carrier protein [Streptomyces lividans TK24]

Acyl carrier protein [Streptomyces lividans 1326]

actinorhodin polyketide synthase acyl carrier protein [Streptomyces sp. WM6391]

actinorhodin polyketide synthase ACP [Streptomyces coelicolor A3(2)]

MULTISPECIES: actinorhodin polyketide synthase [Streptomyces]

RecName: Full=Actinorhodin polyketide synthase acyl carrier protein; Short=ACP; AltName: Full=actI ORF3

Actinorhodin acyl carrier protein (act ACP) from S. coelicolor was heterologously overexpressed in its apo form in E. coli BL21 (DE3) cells. These cells contained the plasmid pET11c C17S act ACP (courtesy of Dr. Tom Nicholson). This IPTG inducible vector is both easier to use and more reliable than the heat inducible pT7-7 version originally constructed.

Ensemble of 20 structures of malonylated act ACP. Malonyl ACP was synthesized using Malonyl CoA (12C,14N) and holosynthase to load the acylated 4'phosphopantetheine chain onto the ACP.

All 3D samples were performed consecutively in a 2 week run on the spectrometer.

Equipped with a triple resonance XYZ PFG room temperature probe.

1H, 13C and 15N NMR assignments for holo ACP from the actinorhodin polyketide synthase of Streptomyces coelicolor

1H, 13C and 15N NMR assignments for apo ACP from the actinorhodin polyketide synthase of Streptomyces coelicolor

1H, 13C and 15N NMR assignments for acetyl ACP from the actinorhodin polyketide synthase of Streptomyces coelicolor

NMR Solution Structures of butyryl-ACP (a non-polar, non pathway intermediate) from Streptomyces coelicolor

NMR Solution Structures of hexanoyl ACP (a non natural intermediate) from Streptomyces coelicolor

NMR Solution Structures of octanoyl ACP (a non-natural intermediate) from Streptomyces coelicolor

NMR Solution Structures of 3-oxo-butyl-ACP, an intermediate mimic from Streptomyces coelicolor

NMR Solution Structures of 3,5-dioxohexyl ACP (a triketide mimic) from Streptomyces coelicolor

NMR Solution structures of holo ACP from the actinorhodin polyketide synthase of Streptomyces coelicolor

NMR Solutions structure of apo ACP from the actinorhodin pathway of Streptomyces coelicolor

NMR Solution structures of acetyl ACP from the actinorhodin polyketide synthase of Streptomyces coelicolor

13C 15N labelled Sample

Samples were 1mM NaN3, 5%/95% D2O/H2O in 600uL potassium phosphate buffer (20mM, pH 5.5) and 1-2mM derivatised ACP

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax

Department of Biochemistry, University of Cambridge, Cambridge, CB2 1GA, UK; Macromolecular Structure Database, European Bioinformatics Institute, Hinxton, Cambridge, CB10 1SD, UK