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The high-resolution NMR structure of the R21A Spc-SH3:P41 complex: understanding the determinants of binding affinity by comparison with Abl-SH3.

The R21A mutant of the alpha-spectrin SH3 domain (R21A Spc-SH3) in its P41-bound form

2 mM R21A SH3-SH3 sample in 0.500 ml 90% H2O/10% D2O, 20 mM d5-glycine at pH 3.5, 300 K

Bruker DRX 750 MHz spectrometer, TXI-XYZ probe

Processing using NMRPIPE, analysis using NMRVIEW