It should be noted that the x-ray crystal structure of the same protein has
been determined to 1.55A and will be published elsewhere. When coordinates
have been deposited, we would like to add the reference to the PDB file to
this deposition.

The first three N-terminal residues, GAM, were not derived from GMEB1 but from
the linker resulting from cleavage of the N-terminal Histag. Signals for some
residues, mostly occuring in flexible loops or solvent-exposed regions, were
not observed in the NMR spectra and so no chemical shift information is given
for these residues.

Information obtained from PDB's Chemical Component Dictionary
at http://wwpdb-remediation.rutgers.edu/downloads.html
Downloaded on Thu Jun 2 09:48:05 2011

1H, 13C and 15N backbone resonance assignments of the SAND domains from glucocorticoid modulatory element binding proteins-1 and -2.

There is a tripeptide N-terminal tag (GAM) followed by 94 residues of the human
GMEB1 protein, residues Glu 89 to Lys 182. Theoretical pI=9.35.
The protein sequence given should be numbered according to its occurrence in
the wild-type protein. Thus the first residue (G) should be numbered 86, and
the last residue (K) 182. Nevertheless, it should be noted that the first three
residues (GAM) are derived from an N-terminal tag.

Glucocorticoid modulatory element-binding protein 1 (GMEB-1)

Glucocorticoid modulatory element-binding protein 1 (GMEB-1) (Parvovirus initiation factor p96) (PIF p96) (DNA-binding protein p96PIF)

glucocorticoid modulatory element binding protein 1 isoform 2 [Homo sapiens]

glucocorticoid modulatory element binding protein 1 isoform 1 [Homo sapiens]

glucocorticoid modulatory element binding protein 1 [Mus musculus]

glucocorticoid modulatory element binding protein 1 [Bos taurus]

glucocorticoid modulatory element binding protein 1 [Homo sapiens]

DNA binding protein p96PIF [Homo sapiens]

glucocorticoid modulatory element binding protein 1; GMEB-1 [Mus musculus]

glucocorticoid modulatory element binding protein-1 [Homo sapiens]

Crystal Structure Of The Sand Domain From Glucocorticoid Modulatory Element Binding Protein-1 (Gmeb1)

Protein was expressed from an in-house modified pET-24d vector (originally
supplied by NOVAGEN) in E. coli induced with IPTG at 23 degrees C for 12
hours.

Backbone resonance assignments for the human GMEB-1 SAND domain

Samples were also stored under either nitrogen or argon gas in order to limit
oxidation of the numerous Cys residues (important in zinc-binding).