Chain A, Solution Structure Of Gaip (Galpha Interacting Protein): A Regulator Of G Protein Signaling

de Alba, E., De Vries, L., Farquhar, M., and Tjandra, N., "Solution structure
of human GAIP (G alpha interacting protein). A regulator of G protein
signaling," J. Mol. Biol. 291, 927-939 (1999).

Solution structure of human GAIP (G alpha interacting protein). A regulator of G
protein signaling

The solution structure of the human protein GAIP (Galpha interacting protein), a
regulator of G protein signaling, has been determined by NMR techniques.
Dipolar couplings of the oriented protein in two different liquid crystal media
have been used in the structure calculation. The solution structure of GAIP is
compared to the crystal structure of an homologous protein from rat (RGS4)
complexed to the alpha-subunit of a G protein. Some of RGS4 residues involved
in the Galpha-RGS binding interface have similar orientations in GAIP (free
form), indicating that upon binding these residues do not suffer conformational
rearrangements, and therefore, their role does not seem to be restricted to
Galpha interaction but also to RGS folding and stability. We suggest that other
structural differences between the two proteins may be related to the process
of binding as well as to a distinct efficiency in their respective GTPase
activating function.

Solution structure of human GAIP (Galpha interacting protein): a regulator of G protein signaling.

A 153 residue protein. Only 128 residues
correspong to the native sequence of GAIP,
the rest are artifacts from protein engineering

Solution Structure Of Gaip (Galpha Interacting Protein): A Regulator Of G Protein Signaling

Regulator of G-protein signaling 19 [Bos taurus]

regulator of G-protein signaling 19, isoform CRA_a [Mus musculus]

regulator of G-protein signaling 19, isoform CRA_e [Mus musculus]

regulator of G-protein signaling 19-like protein [Camelus ferus]

PREDICTED: regulator of G-protein signaling 19 isoform X1 [Cavia porcellus]

PREDICTED: regulator of G-protein signaling 19 isoform X1 [Otolemur garnettii]

PREDICTED: regulator of G-protein signaling 19 isoform X1 [Bos taurus]

PREDICTED: regulator of G-protein signaling 19 isoform X1 [Bubalus bubalis]

RecName: Full=Regulator of G-protein signaling 19; Short=RGS19

TPA: regulator of G-protein signaling 19 [Bos taurus]

NMR chemical shift assignment of human GAIP (Galpha Interacting Protein): A
regulator of G protein signaling