The E9 DNase forms the cytotoxic domain of colicin E9 and is located at its C-terminus (the last 134 residues). It acts as an endonuclease and is active on both single- and double-stranded DNA but with undefined specificity. Colicins are plasmid-encoded protein antibiotics produced by, and active against, Eschericia coli and closely related bacteria.

Assignments refer to the major conformational species
of E9 DNase.

Assignments refer to the minor conformational species
of E9 DNase.

Whittaker, S. B.-M., Boetzel, R., MacDonald, C. J., Lian, L-Y., James, R.,
Kleanthous, C., and Moore, G. R., "Assignment of 1H, 13C and 15N Signals of
the DNase Domain of Colicin E9," J. Biomol. NMR in preparation.

Assignment of 1H, 13C and 15N signals of the DNase domain of colicin E9.

The NMRPipe system is a UNIX software environment of processing, graphics, and
analysis tools designed to meet current routine and research-oriented
multidimensional processing requirements, and to anticipate and accommodate
future demands and developments. The system is based on UNIX pipes, which allow
programs running simultaneously to exchange streams of data under user control.
In an NMRPipe processing scheme, a stream of spectral data flows through a
pipeline of processing programs, each of which performs one component of the
overall scheme, such as Fourier transformation or linear prediction. Complete
multidimensional processing schemes are constructed as simple UNIX shell
scripts. The processing modules themselves maintain and exploit accurate
records of data sizes, detection modes, and calibration information in all
dimensions, so that schemes can be constructed without the need to explicitly
define or anticipate data sizes or storage details of real and imaginary
channels during processing. The asynchronous pipeline scheme provides other
substantial advantages, including high flexibility, favorable processing
speeds, choice of both all-in-memory and disk-bound processing, easy adaptation
to different data formats, simpler software development and maintenance, and
the ability to distribute processing tasks on multi-CPU computers and computer
networks.

Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J. and Bax, A.
J. Biomol. NMR 6, 277-293 (1995)

NMRPipe: a multidimensional spectral processing system based on UNIX pipes.

Bartels, C., Xia, T.-H., Billeter, M., Guentert, P. & Wuethrich, K.
J. Biomol. NMR 6, 1-10 (1995).

The program XEASY for computer-supported NMR spectral analysis of biological macromolecules.

Eaton, T. & James, R. Nucleic Acids Res. 17, 1761-1761 (1989).
Complete nucleotide sequence of the colicin E9 (cei) gene.

E9 DNase exists as two conformers
in solution (60:40) that undergo slow
chemical exchange.

Crystal Structure Of The Escherichia Coli Colicin E9 Dnase Domain With Its Cognate Immunity Protein Im9

Crystal Structure Of Colicin E9 Dnase Domain With Its Cognate Immunity Protein Im9 (1.7 Angstroms)

Crystal Structure Of The E9 Dnase Domain With A Mutant Immunity Protein Im9(E41a)

Crystal Structure Of The E9 Dnase Domain

Crystal Structure Of The Mutant His103ala Of The Colicin E9 Dnase Domain In Complex With Zn+2 (2.0 Angstroms)

Crystal Structure Of The Colicin E9, Mutant His103ala, In Complex With Mg+2 And Dsdna (Resolution 2.9a)

Crystal Structure Of The Colicin E9, Mutant His103ala, In Complex With Zn+2 And Dsdna (Resolution 2.4a)

Crystal Structure Of The Complex Of The Colicin E9 Dnase Domain With A Mutant Immunity Protein, Imme9 (D51a)

Crystal Structure Of The E9 Dnase Domain With A Mutant Immunity Protein Im9 (Y55a)

Crystal Structure Of The E9 Dnase Domain With A Mutant Immunity Protein Im9 (Y54f)

Crystal Structure Of The E9 Dnase Domain With A Mutant Immunity Protein Im9 (Y55f)

Crystal Structure Of The E9 Dnase Domain With A Mutant Immunity Protein Im9 (V34a)

Crystal Structure Of The E9 Dnase Domain With A Mutant Immunity Protein Im9 (D51a)

Chemical Shift Structure Of Colicin E9 Dnase Domain With Its Cognate Immunity Protein Im9

N75a Mutant Of E9 Dnase Domain In Complex With Im9

K97a Mutant Of E9 Dnase Domain In Complex With Im9

R54a Mutant Of E9 Dnase Domain In Complex With Im9

F86a Mutant Of E9 Dnase Domain In Complex With Im9

The Crystal Structure Of Im2 In Complex With Colicin E9 Dnase

colicin E9 [Escherichia coli str. K-12 substr. W3110]

colcinin E9 C-terminal fragment [Plasmid ColE9-J]

homologous Immunity protein Im9 bound to DNase domain of colicin E9

Im9 bound DNase domain of non cognate binding partner E9

Processing triple resonance data and peak-picking. FELIX ASSIGN: initial semi-automated assignment. In-house developed user macros for data processing.

Defining strips for 3D NOESY to aid sequential assignment.