The assignment is done on the isolated RNase H domain

cleaves the RNA moiety of RNA:DNA hybrids

Kern, G., Handel, T., and Marqusee, S. "Characterization of a Folding
Intermediate from HIV-1 Ribonuclease H," Protein Sci. 7, 2164-2174 (1998).

Protein science : a publication of the Protein Society
Characterization of a folding intermediate from HIV-1 ribonuclease H.

A considerable degree of variability exists in the way that 1H, 13C and 15N
chemical shifts are reported and referenced for biomolecules. In this article
we explore some of the reasons for this situation and propose guidelines for
future chemical shift referencing and for conversion from many common 1H, 13C
and 15N chemical shift standards, now used in biomolecular NMR, to those
proposed here.

Wishart, D. S., Bigam, C. G., Yao, J., Abildgaard, F., Dyson, H. J.,
Oldfield, E., Markley, J. L., and Sykes, B. D.
J. Biomol. NMR 6, 135-140 (1995).

1H, 13C and 15N chemical shift referencing in biomolecular NMR.

The solution structure of the ribonuclease H domain of HIV-1 reverse
transcriptase has been investigated by three-dimensional double and triple
resonance heteronuclear magnetic resonance spectroscopy. The domain studied has
138 residues and comprises residues 427 to 560 of the 66 kDa reverse
transcriptase with an additional four residues at the N terminus. Initial
studies on the wild-type protein were hindered by severe differential line
broadening, presumably due to conformational averaging. Mutation of the single
tryptophan residue located in a loop at position 113 (position 535 in the
reverse transcriptase sequence) to an alanine resulted in much improved
spectral properties with no apparent change in structure. 1H, 15N and 13C
backbone resonances were assigned sequentially using a range of
three-dimensional double and triple resonance heteronuclear experiments on
samples of uniformly (greater than 95%) 15N and 15N/13C-labeled protein, and
the secondary structure was elucidated from a qualitative analysis of data
derived from three-dimensional 15N- and 13C-edited nuclear Overhauser
enhancement spectra. The secondary structure comprises three alpha-helices and
five strands arranged in a mixed parallel/antiparallel beta-sheet with a +1,
+1, -3x, -1x topology. The C-terminal region from residue 114 onwards appears
to be conformationally disordered in solution as evidenced by an almost
complete absence of sequential and medium range nuclear Overhauser effects.

Assignment for a mutant RNase H from HIV-1 strain HXB2 is available
Powers et al. 1991, J. Mol. Biol. 221
1081-90

Secondary structure of the ribonuclease H domain of the human immunodeficiency virus reverse transcriptase in solution using three-dimensional double and triple resonance heteronuclear magnetic resonance spectroscopy.

RNase H domain of HIV-1 Reverse Transcriptase RVT

Hiv-1 Reverse Transcriptase In Complex With The Inhibitor Msc204

The Structure Of Unliganded Reverse Transcriptase From The Human Immunodeficiency Virus Type 1

Structure Of Hiv-1 Reverse Transcriptase In A Complex With The Nonnucleoside Inhibitor Alpha-Apa R 95845 At 2.8 Angstroms Resol

Structure Of Hiv-1 Rt(Slash)tibo R 86183 Complex Reveals Similarity In The Binding Of Diverse Nonnucleoside Inhibitors

Crystal Structure Of The Ribonuclease H Domain Of Hiv-1 Reverse Transcriptase

Human Immunodeficiency Virus Type 1 Reverse Transcriptase Complexed With A 33-Base Nucleotide Rna Pseudoknot

Crystal Structure Of Hiv-1 Reverse Transcriptase In Complex With A Polypurine Tract Rna:dna

K103n Mutant Hiv-1 Reverse Transcriptase In Complex With Efivarenz

Wild Type Hiv-1 Reverse Transcriptase In Complex With Efavirenz

K103n Mutant Hiv-1 Reverse Transcriptase In Complex With The Inhibitor Pnu142721

Hiv-1 Reverse Transcriptase In Complex With The Inhibitor Msc194

Crystal Structure Of Met184ile Mutant Of Hiv-1 Reverse Transcriptase In Complex With Double Stranded Dna Template- Primer

Hiv-1 Reverse Transcriptase Crosslinked To Post- Translocation Aztmp-Terminated Dna (Complex P)

Hiv-1 Reverse Transcriptase Crosslinked To Pre- Translocation Aztmp-Terminated Dna (Complex N)

Crystal Structure Of 3tc-Resistant M184i Mutant Of Hiv-1 Reverse Transcriptase

Crystal Structure Of Hiv-1 Reverse Transcriptase Covalently Tethered To Dna Template-primer Solved To 2.8 Angstroms

Crystallographic Analyses Of An Active Hiv-1 Ribonuclease H Domain Show Structural Features That Distinguish It From The Inacti

Structure Of A Catalytic Complex Of Hiv-1 Reverse Transcriptase: Implications For Nucleoside Analog Drug Resistance

Crystal Structure Of Human Immunodeficiency Virus Type 1 Reverse Transcriptase (Rt) In Complex With Janssen-R100943

Crystal Structure Of Hiv-1 Reverse Transcriptase (Rt) In Complex With Janssen-R147681

Crystal Structure Of Hiv-1 Reverse Transcriptase (Rt) In Complex With Janssen-R129385

Crystal Structure Of Hiv-1 Reverse Transcriptase (Rt) In Complex With Janssen-R120394.

Crystal Structure Of Hiv-1 Reverse Transcriptase (Rt) In Complex With Janssen-R185545

Crystal Structure Of K103n Mutant Hiv-1 Reverse Transcriptase (Rt) In Complex With Janssen-R165335

Hiv-1 Reverse Transcriptase Crosslinked To Tenofovir Terminated Template-Primer (Complex P)

Hiv-1 Reverse Transcriptase Crosslinked To Template-Primer With Tenofovir-Diphosphate Bound As The Incoming Nucleotide Substrat

Hiv-1 Reverse Transcriptase Complexed With Cp-94,707

Crystal Structure Of Hiv-1 Reverse Transcriptase (Rt) In Complex With Janssen-R165481

Crystal Structure Of Hiv-1 Reverse Transcriptase (Rt) In Complex With Thr-50

Crystal Structure Of Hiv-1 Reverse Transcriptase (Rt) In Complex With Janssen-R157208

Crystal Structure Of Hiv-1 Reverse Transcriptase (Rt) In Complex With R221239

Hiv-1 Reverse TranscriptaseFRAGMENT OF FAB 28DNA COMPLEX

Crystal Structure Of Hiv-1 Reverse Transcriptase (Rt) In Complex With Dhbnh, An Rnase H Inhibitor

Crystal Structure Of K103nY181C MUTANT HIV-1 Reverse Transcriptase (Rt) In Complex With Atp

Crystal Structure Of K103nY181C MUTANT HIV-1 Reverse Transcriptase (Rt) In Complex With Nonnucleoside Inhibitor Hby 097

Crystal Structures Of Hiv-1 Reverse Transcriptase Complexes With Thiocarbamate Non-Nucleoside Inhibitors

Crystal Structure Of Hiv-1 Reverse Transcriptase (Rt) In Complex With A Difluoromethylbenzoxazole (Dfmb) Pyrimidine Thioether D

Crystal Structure Of Hiv-1 Reverse Transcriptase (rt) In Complex With Tmc278 (rilpivirine), A Non-nucleoside Rt Inhibitor

Crystal Structure Of L100i/k103n Mutant Hiv-1 Reverse Transcriptase (rt) In Complex With Tmc278 (rilpivirine), A Non-nucleoside

Crystal Structure Of K103n/y181c Mutant Hiv-1 Reverse Transcriptase (rt) In Complex With Tmc278 (rilpivirine), A Non-nucleoside

Crystal Structure Of An Engineered Form Of The Hiv-1 Reverse Transcriptase, Rt69a

Structural Basis Of Asymmetry In The Human Immunodeficiency Virus Type 1 Reverse Transcriptase Heterodimer

Hiv-1 Reverse Transcriptase With The Inhibitor Beta- Thujaplicinol Bound At The Rnase H Active Site

Crystal Structure Of Hiv-1 Reverse Transcriptase (Rt) In Complex With The Non-Nucleoside Rt Inhibitor (E)-S-Methyl 5-(1-(3,7-Di

Crystal Structure Of The Hiv-1 Reverse Transcriptase (Rt) In Complex With The Alkenyldiarylmethane (Adam) Non-Nucleoside Rt Inh

Crystal Structure Of Hiv-1 Rt Bound To A 6-Vinylpyrimidine Inhibitor

Crystal Structure Of The Hiv-1 Reverse Transcriptase Bound To A 6-Vinylpyrimidine Inhibitor

K65r Mutant Hiv-1 Reverse Transcriptase Cross-Linked To Ds-Dna And Complexed With Tenofovir-Diphosphate As The Incoming Nucleot

K65r Mutant Hiv-1 Reverse Transcriptase Cross-Linked To Ds- Dna And Complexed With Datp As The Incoming Nucleotide Substrate

Hiv-1 Reverse Transcriptase Isolated Rnaseh Domain With The Inhibitor Beta-Thujaplicinol Bound At The Active Site

Crystal Structure Of Azt-Resistant Hiv-1 Reverse Transcriptase Crosslinked To A Dsdna With A Bound Excision Product, Aztppppa

Crystal Structure Of Wild-Type Hiv-1 Reverse Transcriptase Crosslinked To A Dsdna With A Bound Excision Product, Aztppppa

Crystal Structure Of Azt-Resistant Hiv-1 Reverse Transcriptase Crosslinked To Pre-Translocation Aztmp-Terminated Dna (Complex N

Crystal Structure Of Azt-resistant Hiv-1 Reverse Transcriptase Crosslinked To Post-translocation Aztmp-terminated Dna (complex

Crystal Structure Of Unliganded Azt-Resistant Hiv-1 Reverse Transcriptase

P15 Hiv Rnaseh Domain With Inhibitor Mk3

Hiv-1 Reverse Transcriptase In Complex With Manicol At The Rnase H Active Site And Tmc278 (rilpivirine) At The Nnrti Binding Po

Crystal Structure Of Hiv-1 Reverse Transcriptase (rt) In Complex With Tsao-t, A Non-nucleoside Rt Inhibitor (nnrti)

Crystal Structure Of Hiv-1 Reverse Transcriptase (Rt) With Dna And Azttp

Crystal Structure Of Hiv-1 Reverse Transcriptase (Rt) Cross-Linked With Azt-Terminated Dna

Crystal Structure Of Hiv-1 Reverse Transcriptase (Rt) With Dna And The Nonnucleoside Inhibitor Nevirapine

Crystal Structure Of Hiv-1 Reverse Transcriptase (Rt) With Polymorphism Mutation K172a And K173a

Crystal Structure Of Hiv-1 Reverse Transcriptase (rt) In Complex With Rilpivirine (tmc278, Edurant), A Non-nucleoside Rt-inhibi

Crystal Structure Of Hiv-1 Reverse Transcriptase In Complex With (E)- 3-(3-Chloro-5-(4-Chloro-2-(2-(2,4-Dioxo-3,4- Dihydropyrim

Crystal Structure Of Hiv-1 Reverse Transcriptase (Rt) In Complex With (E)-3-(3-(2-(2-(2,4-Dioxo-3,4-Dihydropyrimidin-1(2h)-Yl)e

Crystal Structure Of Hiv-1 Reverse Transcriptase In Complex With Rilpivirine (tmc278) Based Analogue

Crystal Structure Of K103n/y181c Mutant Of Hiv-1 Reverse Transcriptase In Complex With Rilpivirine (tmc278) Analogue

Hiv-1 Reverse Transcriptase With Bound Fragment At The Incoming Dntp Binding Site

Hiv-1 Reverse Transcriptase With Bound Fragment At The Rnase H Primer Grip Site

Hiv-1 Reverse Transcriptase With Bound Fragment At The 428 Site

Detecting Allosteric Sites Of Hiv-1 Reverse Transcriptase By X-ray Crystallographic Fragment Screening

Hiv-1 Reverse Transcriptase With Bound Fragment At The Knuckles Site

Hiv-1 Reverse Transcriptase With Bound Fragment At The 507 Site

Hiv-1 Reverse Transcriptase With Bound Fragment Near Knuckles Site

Hiv-1 Reverse Transcriptase With Bound Fragment At Nnrti Adjacent Site

Crystal Structure Of Hiv-1 Reverse Transcriptase In Complex With 4- ((4-methoxy-6-(2-morpholinoethoxy)-1,3,5-triazin-2-yl)amino

Crystal Structure Of Hiv-1 Reverse Transcriptase (rt) In Complex With An Anilinylpyrimidine Derivative (jlj-135)

Crystal Structure Of Hiv-1 Reverse Transcriptase In Complex With (e)- 3-(3-(4-chloro-2-(2-(2,4-dioxo-3,4-dihydropyrimidin-1(2h)

Crystal Structure Of Hiv-1 Reverse Transcriptase In Complex With (e)- 3-(3-bromo-5-(4-chloro-2-(2-(2,4-dioxo-3,4-dihydropyrimid

Crystal Structure Of Hiv-1 Reverse Transcriptase In Complex With 8-(2- (2-(2,4-dioxo-3,4-dihydropyrimidin-1(2h)-yl)ethoxy)pheno

Crystal Structure Of Hiv-1 Reverse Transcriptase In Complex With 4- ((4-(mesitylamino)-6-(3-morpholinopropoxy)-1,3,5-triazin-2-

Crystal Structure Of Hiv-1 Reverse Transcriptase In Complex With 4- ((4-(mesitylamino)-1,3,5-triazin-2-yl)amino)benzonitrile (j

Crystal Structure Of Hiv-1 Reverse Transcriptase In Complex With Rna/dna And Datp

Crystal Structure Of Hiv-1 Reverse Transcriptase In Complex With Rna/dna And Nevirapine

Crystal Structure Of Hiv-1 Reverse Transcriptase In Complex With Bulge-rna/dna And Nevirapine

Crystal Structure Of Hiv-1 Reverse Transcriptase In Complex With Gap- Rna/dna And Nevirapine

Structure Of A Dihydroxycoumarin Active-site Inhibitor In Complex With The Rnase H Domain Of Hiv-1 Reverse Transcriptase

Crystal Structure Of Hiv-1 Reverse Transcriptase (rt) With Dna And A Nucleoside Triphosphate Mimic Alpha-carboxy Nucleoside Pho

Crystal Structure Of Hiv-1 Reverse Transcriptase (k103n,y181c) Variant In Complex With (e)-3-(3-chloro-5-(4-chloro-2-(2-(2,4-di

Crystal Structure Of Hiv-1 Reverse Transcriptase (y181c) Variant In Complex With (e)-3-(3-chloro-5-(4-chloro-2-(2-(2,4-dioxo-3,

Crystal Structure Of Hiv-1 Reverse Transcriptase (k103n, Y181c) Variant In Complex With (e)-3-(3-chloro-5-(2-(2-(2,4-dioxo-3,4-

Crystal Structure Of Hiv-1 Reverse Transcriptase In Complex With (e)- 3-(3-chloro-5-(2-(2-(2,4-dioxo-3,4-dihydropyrimidin-1(2h)

Crystal Structure Of Hiv-1 Reverse Transcriptase (y181c) Variant In Complex With (e)-3-(3-chloro-5-(2-(2-(2,4-dioxo-3,4-dihydro

Crystal Structure Of Hiv-1 Reverse Transcriptase In Complex With 5-(2- (2-(2,4-dioxo-3,4-dihydropyrimidin-1(2h)-yl)ethoxy)pheno

Crystal Structure Of Hiv-1 Reverse Transcriptase In Complex With 7- ((4-((4-cyanophenyl)amino)-1,3,5-triazin-2-yl)amino)-6,8- D

Crystal Structure Of Hiv-1 Reverse Transcriptase In Complex With 6- ((4-((4-cyanophenyl)amino)-1,3,5-triazin-2-yl)amino)-5,7-di

Crystal Structure Of Hiv-1 Reverse Transcriptase (k101p) Variant In Complex With 8-(2-(2-(2,4-dioxo-3,4-dihydropyrimidin-1(2h)-

Structure Of Hiv-1 Reverse Transcriptase Bound To A Novel 38-mer Hairpin Template-primer Dna Aptamer

pol polyprotein, partial [Human immunodeficiency virus 1]

reverse transcriptase, RT [human immunodeficiency virus type 1 HIV-1, zidovudine (AZT)-sensitive, Peptide, 559 aa]

truncated pol protein [synthetic construct]

RecName: Full=Gag-Pol polyprotein; AltName: Full=Pr160Gag-Pol; Contains: RecName: Full=Matrix protein p17; Short=MA; Contains:

native (The gene was synthesized and cloned into a pAED4 vector under the control of a
T7 promotor)

Family: Retroviridae, Genus: lentivirus, Type of species: HIV-1,
Reference Strain: ICTVdb decimal code is 61.0.6.5.001

Human immunodeficiency virus type 1 BH10

Backbone 1H, 13C and 15N Chemical Shift Assignment for the HIV-1 RNase H Domain