Tolerance values used in automated NOESY assignments.

Lys42 acetylated mature SMALL UBIQUITIN-RELATED MODIFIER 2 (SUMO2)
Residues 1-14 (MGSSHHHHHHSQDP) represent a non-native purification tag. These residues were neither assigned nor included in structure calculation.

PLASMID PCDF PYLT-1 WITH SUMO INSERT WITH K42STOP MUTATION (WITH AMBER CODON) AND PACKRS-3 AS DESCRIBED IN NEUMANN ET AL., MOL CELL, 36, 153, 2009

SOLUTION STRUCTURE OF LYS42 ACETYLATED HUMAN SUMO2

NMR DATA WAS ACQUIRED AT 295K USING SHIGEMI NMR TUBES.

TORSION ANGLE DYNAMICS, DGSA- DISTANCE GEOMETRY SIMULATED ANNEALING

SOLUTION STRUCTURE OF LYS37 ACETYLATED HUMAN SUMO1

SOLUTION STRUCTURE OF LYS33 ACETYLATED HUMAN SUMO2

SOLUTION STRUCTURE OF LYS39 ACETYLATED HUMAN SUMO1

1 mM [U-100% 13C; U-100% 15N; U-80% 2H] SUMO2 K42Ac, 10 mM potassium phosphate, 100 mM potassium chloride, 2 mM DTT, 0.1 mM EDTA, 0.001 % sodium azide, 90% H2O/10% D2O

1 mM [U-100% 15N] SUMO2 K42Ac, 10 mM potassium phosphate, 100 mM potassium chloride, 2 mM DTT, 0.1 mM EDTA, 0.001 % sodium azide, 90% H2O/10% D2O

1 mM [U-100% 15N] SUMO2 K42Ac, 10 mM potassium phosphate, 100 mM potassium chloride, 2 mM DTT, 0.1 mM EDTA, 0.001 % sodium azide, 10 mg/mL Pf1 phage, 90% H2O/10% D2O

1 mM K42Ac SUMO2, 10 mM potassium phosphate, 100 mM potassium chloride, 2 mM DTT, 0.1 mM EDTA, 0.001 % sodium azide, 90% H2O/10% D2O

Schwieters, Kuszewski, Tjandra and Clore