Double-stranded RNA-binding protein Staufen homolog 1/RNA Complex

2D 1H-1H, 13C 1F-filtered 2F-filtered NOESY

Staufen1 reads out structure and sequence features in ARF1 dsRNA for target recognition.

Solution structure of Staufen1 dsRBD4 - hARF1 SBS dsRNA complex.

310 uM [U-15N] Staufen1 dsRBD4, 310 uM Unlabelled hARF1, 90-95% H20/5-10% D20

405 uM [U-13C; U-15N] Staufen1 dsRBD4, 405 uM Unlabelled hARF1, 90-95% H20/5-10% D20

354 uM [U-15N] Staufen1 dsRBD4, 354 uM [U-13C] hARF1, 100% D2O

451 uM [U-13C; U-15N] Staufen1 dsRBD4, 451 uM Unlabelled hARF1, 100% D2O

Laskowski, MacArthur, Smith, Jones, Hutchinson, Morris, Moss and Thornton

Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax