Unravelling the structure and dynamics of the human DNAJB6b chaperone by NMR reveals insights into Hsp40-mediated proteostasis

Solution NMR structure of the DNAJB6b deltaST variant (Aligned on the J domain)

2e-04 M [U-13C; U-15N; U-2H] DNAJB6b deltaST variant.
This variant is missing residues 132-183 in comparison to the full-length DNAJB6b,
5e-05 M sodium chloride, 0.02 % w/v sodium azide, 0.02 M sodium phosphate,
90% H2O/10% D2O

2e-04 M [ILV-CH3; U-13C; U-15N; U-2H] DNAJB6b deltaST variant.
This variant is missing residues 132-183 in comparison to the full-length DNAJB6b,
0.05 M sodium chloride, 0.02 % w/v sodium azide, 0.02 M sodium phosphate, 100% D2O

2e-04 M [ILV-CH3; U-15N; U-2H] DNAJB6b deltaST variant.
This variant is missing residues 132-183 in comparison to the full-length DNAJB6b,
0.05 M sodium chloride, 0.02 % w/v sodium azide, 0.02 M sodium phosphate, 100% D2O

2e-04 M [U-13C; U-15N] DNAJB6b deltaST variant.
This variant is missing residues 132-183 in comparison to the full-length DNAJB6b,
0.05 M sodium chloride, 0.02 % w/v sodium azide, 0.02 M sodium phosphate, 90% H2O/10% D2O

2e-04 M [U-15N;U-2H] DNAJB6b deltaST variant.
This variant is missing residues 132-183 in comparison to the full-length DNAJB6b,
0.05 M sodium chloride, 0.02 % w/v sodium azide, 0.02 M sodium phosphate, 90% H2O/10% D2O

2e-04 M [U-15N;U-2H] DNAJB6b deltaST variant.
This variant is missing residues 132-183 in comparison to the full-length DNAJB6b,
0.1 M sodium chloride, 0.02 % w/v sodium azide, 0.02 M sodium phosphate, 90% H2O/10% D2O

2e-04 M [U-15N;U-2H] DNAJB6b deltaST variant.
This variant is missing residues 132-183 in comparison to the full-length DNAJB6b,
0.05 M sodium chloride, 0.02 % w/v sodium azide, 2e-05 M sodium phosphate, 90% H2O/10% D2O

Schwieters, Kuszewski, Tjandra and Clore

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax