NMR structure of foldswitch-stablized KaiB from Thermosynechococcus elongatus

Y8A, Y94A, N29A, G89A, D91R, FLAG tag C-terminus insertion, C-terminal truncation after Residue D99

Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Circadian clock protein KaiB in complex with pseudo receiver domain of CikA

800 uM [U-99% 13C; U-99% 15N] KaiB mutant in foldswitched state, 95% H2O/5% D2O

800 uM [U-99% 13C; U-99% 15N] KaiB mutant in foldswitched state, 99.96% D2O

300 uM [U-99% 13C; U-99% 15N] KaiB mutant in foldswitched state, 90% H2O/10% D2O

356 uM [U-99% 13C; U-99% 15N] KaiB mutant in foldswitched state, 90% H2O/10% D2O

Garrett DS, Powers R, Gronenborn AM, Clore GM. J Magn Reson. 2011 Dec;213(2):357-63. doi: 10.1016/j.jmr.2011.09.007. A common sense approach to peak picking in two-, three-, and four-dimensional spectra using automatic computer analysis of contour diagrams http://spin.niddk.nih.gov/dgarrett/Xipp/xipp.html

Schwieters, Kuszewski, Tjandra and Clore

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax