Phosphorylation of Mdm2 by DNA-Damage Response Kinases in physiological conditions monitored using 13C-NMR

The following residues are phosphorylated : T351, S386, S388, S395, S402, S407, T419, S429.

The following residues are phosphorylated : T351, S386, S388, S395, S402, S407, T419, S429.
Phosphorylation was obtained upon reaction with purified, commercial DNA-PK

Human Mdm2 fragment 284-434, C362A-C374A; phosphorylated on T351, S386, S388,
S395, S402, S407, T419 and S429 by DNA-PK (DNA-PKc+Ku70+Ku80+calf thymus DNA);
contains a zinc-finger domain (293-333) showing two stable conformations
(Prolines in trans or in cis) as reported previously in the litterature ; we
report here only the trans conformation assignment.

Backbone 1H, 13C, and 15N Chemical Shift Assignments for human Mdm2 (residues 284 to 434) phosphorylated by DNA-PK

DNA-PK, ATP and Mg2+ were removed from the sample using size-exclusion chromatography.