Two Rad50 NBD subunits bound to a Mre11 dimer

Adjacent mutations in the archaeal Rad50 ABC ATPase D-loop disrupt allosteric regulation of ATP hydrolysis through different mechanisms.

mutated to add TEV cleavable His-Tag on N-terminus

ILVM methyl chemical shifts for Pyrococcus furiosus Rad50 NBD (L828F) with unlabeled Mre11

MR complex [Rad50 NBD (wild type) with unlabeled Mre11]

MR complex [Rad50 NBD (D829N) with unlabeled Mre11]