Methionine in a protein hydrophobic core drives tight interactions required for assembly of spider silk.

N-terminal domain (NTD) of major ampullate spidroin 1 protein
The first two amino acids in the protein sequence (GS) are remains of a thrombin cleavage site.

plasmid encodes for a His-tag with thrombin cleavage site

Euprosthenops australis major ampullate spidroin 1 N-terminal domain (NTD) at pH7 with mutations M20L, M24L, M41L, M48L, M77L, M101L.
Euprosthenops australis major ampullate spidroin 1 N-terminal domain (NTD) mutant at pH7

Center for Biomolecular Resonance (BMRZ) Frankfurt

TORSION ANGLE DYNAMICS ONFORMERS, NUMBER CALCULATED : NULL