Nisin:Lipid II pores in lipid membranes.
A stoichiometry of 8:4 Nis:LII units is assumed.

The molecular complex forms pores in bacterial membranes, disrupting the membranes.

This entry displays the 1:1 Nis:LII complex structure calculated in DMSO

FID resolution. On specific cases, line-broadening effects

(2R)-2-[(2S,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxypropanoic acid

High-resolution NMR studies of antibiotics in cellular membranes.

Thioether bonds (-S-) instead of dissulfide bonds (-S=S-) are present.

The X referes to Dehydrobutyrine, Dehydroalanine, D-AlalineS and AlanineS (Lanthionine)
and Aminobutyric acid and AlanineS (3-Methyllanthionine).
The CYS residues 7, 11, 19, 26, 28 should be regarded as an alanine crosslinked via a sulfour atom.

Synthesised in isolated M. flavus cellular membranes

1H, 15N and 13C chemical shifts of Nisin bound to Lipid II in lipid membranes.

Solid-state NMR, dipolar-based experiments

Samples measured with a 1.3mm rotor at 60 kHz MAS and 3.2mm rotor at 17 kHz MAS.
Cooling was employed to achieve desired temperature.