Cohesin domain 5 is part of a large protein called "Scaffolding" which in turn
is connected by non-covalent interactions to dockerin proteins and attached
hydrolytic enzymes, as well as to the cell wall. The linker sequence follows the
folded cohesin domain in the WT protein except the linker has an extra Gly at
the N-terminus and the peptide was acetylated at the N-terminus and amidated at
the C-terminus (residues 146-169).

Forms part of the structural core of the scaffoldin complex of the cellulosome.

The uncertainty for 13C alpha and beta is 0.05 ppm, but the uncertainty for 13CO is lower; it is equal to 0.01 ppm.
standard deviation of multiple assignments.

1H directly referenced to upfield tri-methyl group of DSS.
13C and 15N nuclei are indirectly referenced using their
respective gyromagnetic ratios to 1H as recommended by
Markley, Wuthrich, et al.

Solution conformation of a cohesin module and its scaffoldin linker from a prototypical cellulosome.

Coheson Domain 5 from the Clostridium themocellum cellulosome
Coheson Domain 5 from Clostridium themocellum

equipped with 15N,1H, 13C cryoprobe and Z-gradients